ABSTRACT
Objective
The
aim
of
this
study
is
to
explore
the
mechanism
benzylurea
in
inflammatory
injury
human
periodontal
ligament
fibroblasts
(hPDLFs).
Methods
An
inflammation
model
hPDLFs
was
established
using
LPS.
Nuclear
transport
nuclear
transcription
factor‐κB
(NF‐κB),
secretion
cytokines,
and
morphology
distribution
F‐actin
were
determined.
Mitochondrial
function
assessed
by
measuring
mitochondrial
membrane
potential
(MMP),
permeability
transition
pore
(mPTP),
reactive
oxygen
species
(ROS)
levels.
expression
carrier
homolog
2
(MTCH2)
Cytochrome
b5
type
B
(CYB5B)
detected.
Results
Benzylurea
alleviated
effects
lipopolysaccharide
(LPS)
on
proliferation
apoptosis
hPDLFs.
It
reduced
release
cytokines
inhibited
NF‐κB
translocation.
improved
regulating
MMP
preventing
excessive
mPTP
opening.
Furthermore,
LPS
elevated
MTCH2
CYB5B
However,
these
can
be
benzylurea.
altered
directly
affected
expression,
activation
translocation
NF‐κB.
Conclusion
may
act
as
an
effector
MTCH2,
with
enhancing
protecting
from
LPS‐induced
through
MTCH2.
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 14, 2024
Abstract
Targeted
covalent
inhibitors
are
promising
for
drug
discovery
and
challenged
by
the
strict
structural
features
target
proteins
to
sustain
proximity‐induced
conjugations.
Herein,
an
assembly‐glued
strategy
is
reported
create
surface‐displayed
supramolecular
that
leverage
display‐induced
proximity
between
noncovalent
ligands
warheads.
The
ligand
warhead
obtained
via
attaching
epidermal
growth
factor
receptor
(EGFR)‐binding
segment
or
nitroreductase
(NTR)‐activated
moiety
bola‐amphiphilic
peptides,
respectively.
Co‐assembling
with
a
filler
peptide
glues
them
forms
inhibitor.
While
associates
EGFR,
undergoes
NTR‐induced
cysteine
conjugation
facilitated
ligand‐EGFR
association.
In
vitro
studies
show
reliable
hypoxia‐activated
inhibition
of
EGFR
phosphorylation
enhanced
cytotoxicity
inhibitor
under
hypoxic
condition.
addition,
this
also
allows
creating
targeting
without
small
molecular
drugs,
due
vivo
results
illustrate
prolonged
retention
its
efficacy
in
inhibiting
tumor
growth.
These
findings
demonstrate
simultaneous
surface
display
warheads
assembly
adhesives
implementation
functions,
thus
providing
new
developing
future.
Supramolecular
structures
are
widespread
in
living
system,
which
usually
spatiotemporally
regulated
by
sophisticated
metabolic
processes
to
enable
vital
biological
functions.
Inspired
tremendous
efforts
have
been
made
realize
spatiotemporal
control
over
the
self-assembly
of
supramolecular
materials
synthetic
scenario
coupling
chemical
reaction
with
molecular
process.
In
this
review,
we
focused
on
works
related
hydrogels
that
space
and
time
using
reaction.
Firstly,
summarized
how
spatially
controlled
can
be
achieved
via
reaction-instructed
self-assembly,
application
such
a
methodology
biotherapy
was
discussed
as
well.
Second,
reviewed
dynamic
dictated
networks
evolve
their
properties
against
time.
Third,
recent
progresses
both
though
reaction-diffusion-coupled
approach.
Finally,
provided
perspective
further
development
future.
Abstract
Dissipative
self-assembly,
which
exploits
energy
inputs
of
chemical
fuels
to
maintain
the
functional
states
far
from
equilibrium,
is
essential
living
systems.
Among
a
variety
fuels,
carbon
dioxide
(CO2)
gas,
as
one
most
ubiquitous
but
original
forms
fuel
on
life
depends,
has
yet
been
introduced
in
artificial
dissipative
materials.
Here
we
describe
CO2-fueled
non-equilibrium
co-assembly
system
that
couples
with
C1
catalytic
feedback
path
drive
dissipation
and
function
output.
Using
common
frustrated
Lewis
pair
(FLP)
precursors,
CO2
can
dynamically
bridge
between
them
constitute
metastable
amphiphiles,
not
only
highly
activate
also
enable
their
substrates
into
transient
fibrillar
gel.
In
turn,
process
realized
by
cooperative
insertion
owing
proximity
substrate
activated
species
assembled
state.
This
boost
depletion
gas
facilitate
disassembly
sol.
Moreover,
tailoring
intrinsic
substrate/FLP
chemistries,
well
external
cues,
shift
activity
accessible
regulate
period
lifetime
sol-gel-sol
transition
over
wide
range.
Based
tunability
phase
time
scale,
develop
time-dependent
information
encryption
materials
using
FLP
array
loaded
gas-encoded
substrates,
correct
be
read
at
specified
window.
study
provides
inspiration
new
paradigm
for
intelligent
material
applications.
Angewandte Chemie,
Год журнала:
2024,
Номер
136(48)
Опубликована: Авг. 22, 2024
Abstract
Natural
dissipative
assembly
(DSA)
often
exhibit
energy‐driven
shifts
in
natural
functions.
However,
creating
man‐made
DSA
that
can
mimic
such
biological
activities
transformation
remains
relatively
rare.
Herein,
we
introduce
a
cytomembrane‐like
system
based
on
chiral
supramolecules.
This
employs
benzoyl
cysteine
an
out
of
equilibrium
manner,
enabling
the
biofunctions
while
minimizing
material
use.
Specifically,
aroyl‐cystine
derivatives
primarily
assemble
into
stable
M‐helix
nanofibers
under
conditions.
These
enhance
fibroblast
adhesion
and
proliferation
through
stereospecific
interactions
with
cellular
membranes.
Upon
addition
chemical
fuels,
these
functional
temporarily
transform
non‐equilibrium
nanospheres,
facilitating
efficient
drug
delivery.
Subsequently,
nanospheres
revert
to
their
original
nanofiber
state,
effectively
recycling
drug.
The
programmable
function‐shifting
ability
this
establishes
it
as
novel,
fuel‐driven
delivery
vehicle.
And
bioactive
not
only
addresses
gap
synthetic
DSAs
within
applications
but
also
sets
stage
for
innovative
designs
′living′
materials.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(35), С. 45821 - 45829
Опубликована: Авг. 23, 2024
In
situ
self-assembly
in
living
systems
is
referred
to
as
the
processes
that
regulate
assembly
by
stimuli-responsive
reactions
at
target
sites
under
physiological
conditions.
Due
advantages
of
precisely
forming
well-defined
nanostructures
pathological
lesions,
situ-formed
assemblies
with
tailored
bioactivity
are
promising
for
development
next-generation
biomedical
agents.
this
Perspective,
we
summarize
progress
peptides
cells
an
emphasis
on
state-of-the-art
strategies
regulating
processes,
establishing
complexity
within
systems,
and
exploiting
their
applications
biomedicines.
We
also
provide
our
forward
conceiving
perspectives
challenges
demonstrate
its
great
potential
creating
biomaterials
healthcare
future.
ABSTRACT
Objective
The
aim
of
this
study
is
to
explore
the
mechanism
benzylurea
in
inflammatory
injury
human
periodontal
ligament
fibroblasts
(hPDLFs).
Methods
An
inflammation
model
hPDLFs
was
established
using
LPS.
Nuclear
transport
nuclear
transcription
factor‐κB
(NF‐κB),
secretion
cytokines,
and
morphology
distribution
F‐actin
were
determined.
Mitochondrial
function
assessed
by
measuring
mitochondrial
membrane
potential
(MMP),
permeability
transition
pore
(mPTP),
reactive
oxygen
species
(ROS)
levels.
expression
carrier
homolog
2
(MTCH2)
Cytochrome
b5
type
B
(CYB5B)
detected.
Results
Benzylurea
alleviated
effects
lipopolysaccharide
(LPS)
on
proliferation
apoptosis
hPDLFs.
It
reduced
release
cytokines
inhibited
NF‐κB
translocation.
improved
regulating
MMP
preventing
excessive
mPTP
opening.
Furthermore,
LPS
elevated
MTCH2
CYB5B
However,
these
can
be
benzylurea.
altered
directly
affected
expression,
activation
translocation
NF‐κB.
Conclusion
may
act
as
an
effector
MTCH2,
with
enhancing
protecting
from
LPS‐induced
through
MTCH2.
