Synergistic Effect of Cyclodextrins and Electrolytes at High Concentrations on Protein Aggregation Inhibition

Journal of Pharmaceutical Sciences, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

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Impact of arginine addition on protein concentration via ultrafiltration

Protein Expression and Purification, Год журнала: 2025, Номер unknown, С. 106701 - 106701

Опубликована: Март 1, 2025

Язык: Английский

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A Convenient Strategy for Studying Antibody Aggregation and Inhibition of Aggregation: Characterization and Simulation

Pharmaceutics, Год журнала: 2025, Номер 17(4), С. 534 - 534

Опубликована: Апрель 19, 2025

Background/Objectives: Protein aggregation, particularly the aggregation of antibody-based drugs, has long been a significant challenge in downstream processes and formulation. While inhibitory effects excipients on have extensively studied using early experimental characterization methods, complete formulation research requires amounts antibodies time, resulting high costs. Methods: This study proposed quick small-scale position-restrained simulation method which elucidated mechanism reversible self-association (RSA) influence RSA under different conditions. We also validated rationality rapid simulations through long-term (>1 μs) large-scale (>1,000,000 atoms) simulations. Results: Through combing with simple stability characterization, monomer residual content trend shown concentration changes after thermal incubation were found to be similar those observed Additionally, by was characterization. Conclusions: Simulations experiments revealed showed consistent trends, providing better understanding for research.

Язык: Английский

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Uncovering the Pathway of Serine Octamer Magic Number Cluster Formation during Electrospray Ionization: Experiments and Simulations

Journal of the American Chemical Society, Год журнала: 2024, Номер unknown

Опубликована: Сен. 17, 2024

Electrospray ionization (ESI) of serine (Ser) solution generates Ser

Язык: Английский

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Current advances in separation chemistry for antibody purification and analysis

Analytical Sciences, Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

Язык: Английский

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Mechanistic insights into arginine-mediated gluten solubility enhancement and aggregation inhibition across specific subunit and molecular scales

Food Hydrocolloids, Год журнала: 2025, Номер unknown, С. 111451 - 111451

Опубликована: Апрель 1, 2025

Язык: Английский

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A comprehensive review on freeze‐induced deterioration of frozen egg yolks: Freezing behaviors, gelation mechanisms, and control techniques

Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2024, Номер 23(5)

Опубликована: Сен. 1, 2024

Abstract Over the years, production of eggs has increased tremendously, with an estimated global egg 9.7 billion by 2050. Further processing shell to products gained growing popularity. Liquid yolks, innovative form replacement, still suffer from short shelf‐life issues, and freezing been applied maintain freshness. An undesirable phenomenon called “gelation” was found during frozen which attracted numerous scholars study its mechanism quality control methods. Therefore, we comprehensively reviewed history studies on including procedure, fundamentals freezing, gelation mechanism, factors affecting behaviors, techniques behaviors yolks. Reporting procedure yolks will give readers a better understanding science technological aspects Furthermore, comprehensive summary yolk gel formation induced freeze–thawing relevant provide insights researchers manufacturers in field processing.

Язык: Английский

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Residue-resolved insights into the stabilization of therapeutic proteins by excipients: A case study of two monoclonal antibodies with arginine and glutamate

mAbs, Год журнала: 2024, Номер 16(1)

Опубликована: Ноя. 14, 2024

Protein formulation development relies on the selection of excipients that inhibit protein–protein interactions preventing aggregation. Empirical strategies involve screening many excipient and buffer combinations by physicochemical characterization using forced degradation or temperature-induced stress, mostly under accelerated conditions. Such methods do not readily provide information inter- intramolecular responsible for effects excipients. Here, we describe a combined experimental computational approach investigating effect protein–excipient stability, which allows identification preferential interaction sites thus can aid in to be experimentally screened. Model systems composed two marketed therapeutic IgG1 monoclonal antibodies with identical Fc domain sequences, trastuzumab omalizumab, were investigated commonly used arginine, glutamate, equimolar arginine/glutamate mixtures. Protein–excipient studied all-atom molecular dynamics (MD) simulations, show accumulation at specific antibody regions. Preferential excipient-interaction particularly found charged aromatic residues complementary-determining regions, more pronounced arginine contacts omalizumab than trastuzumab. These findings are line stabilizing observed long-term storage stability study. Furthermore, aggregation solubility propensity predicted silico tools align identified MD suggesting different mechanisms play.

Язык: Английский

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Analysis of interactions between amino acids and monolayers of charged side chains

RSC Applied Interfaces, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 18, 2024

The report on an analytical platform for amino acid–amino acid interactions between charged acids and accumulated side chains self-assembled monolayers combined with field-effect transistors.

Язык: Английский

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