Structural and Mechanistic Insights into Oxidative Biaryl Coupling to form Arylomycin Core by an Engineered CYP450 DOI
Vandana Kardam, Vaibhav D. Bhatt, Kshatresh Dutta Dubey

и другие.

Dalton Transactions, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Arylomycin, a potent antibiotic targeting bacterial signal peptidase, is difficult to synthesize experimentally due its poor moderate yields and the formation of mixture compounds. A recent experimental bioengineering work shows that core arylomycin can be efficiently synthesized by engineering cytochrome P450 enzyme from

Язык: Английский

Multifunctional Cytochrome P450 Orchestrates Radical Cleavage and Non-radical Cyclization in 5-Oxaindolizidine Biosynthesis DOI Creative Commons

Kaijin Zhang,

Jingxian Sun, Wei Song

и другие.

Chemical Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Biosynthesis of penicilactam A (1) reveals a multifunctional P450 PnltC catalyzing radical C–C bond cleavage and iminium involved non-radical cyclization.

Язык: Английский

Процитировано

0
Applications of cytochrome P450 monooxygenases in the biosynthesis of active pharmaceutical ingredients DOI Creative Commons
Yuben Qiao, Xiaodong Zhang, Qianqian Xu

и другие.

Green Synthesis and Catalysis, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Assessing the ability of ChatGPT to extract natural product bioactivity and biosynthesis data from publications DOI Creative Commons

Thomas L. Kalmer,

Christine Mae F. Ancajas,

Zihao Cheng

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 2, 2024

Abstract Natural products are an excellent source of therapeutics and often discovered through the process genome mining, where genomes analyzed by bioinformatic tools to determine if they have biosynthetic capacity produce novel or active compounds. Recently, several been reported for predicting natural product bioactivities from sequence gene clusters that them. These potential accelerate rate drug discovery enabling prioritization more likely compounds with therapeutically relevant bioactivities. However, these severely limited a lack training data, specifically data pairing activity labels their products. There many reports in literature not included existing databases. Manual curation is time consuming inefficient. Recent developments large language models chatbot interfaces built on top them enabled automatic extraction text, including scientific publications. We investigated how accurate ChatGPT at extracting necessary predict clusters. found did well determining paper described information about product’s bioactivity. perform as accession numbers cluster producer’s although using altered prompt improved accuracy.

Язык: Английский

Процитировано

1
Loss of fluorine during crosslinking by the biarylitide P450Blt proceeds due to restricted peptide orientation DOI
Yongwei Zhao,

Jemma Gullick,

Mathias H. Hansen

и другие.

Chemical Communications, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

The biarylitide crosslinking enzyme P450

Язык: Английский

Процитировано

1
Mechanistic Perspective on C–N and C–S Bond Construction Catalyzed by Cytochrome P450 Enzymes DOI
Tai‐Ping Zhou,

Yakun Fan,

Jinyan Zhang

и другие.

ACS Bio & Med Chem Au, Год журнала: 2024, Номер 5(1), С. 16 - 30

Опубликована: Ноя. 27, 2024

Cytochrome P450 enzymes catalyze a large number of oxidative transformations that are responsible for natural product synthesis. Recent studies have revealed their unique ability to the formation C-N and C-S bonds, broadening biosynthetic applications. However, enzymatic mechanisms behind these reactions still unclear. This review focuses on theoretical insights into P450-catalyzed bond formation. The key roles conformational dynamics substrate radicals, influenced by enzyme environment, in modulating selectivity reactivity highlighted. Understanding reaction offers valuable guidance engineering design

Язык: Английский

Процитировано

1
Structural and Mechanistic Insights into Oxidative Biaryl Coupling to form Arylomycin Core by an Engineered CYP450 DOI
Vandana Kardam, Vaibhav D. Bhatt, Kshatresh Dutta Dubey

и другие.

Dalton Transactions, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Arylomycin, a potent antibiotic targeting bacterial signal peptidase, is difficult to synthesize experimentally due its poor moderate yields and the formation of mixture compounds. A recent experimental bioengineering work shows that core arylomycin can be efficiently synthesized by engineering cytochrome P450 enzyme from

Язык: Английский

Процитировано

0