Biomacromolecules,
Год журнала:
2024,
Номер
26(1), С. 670 - 678
Опубликована: Дек. 19, 2024
Hydrogel
three-dimensional
(3D)
printing
has
emerged
as
a
highly
valuable
fabrication
tool
for
applications
ranging
from
electronics
and
biomedicine.
While
conventional
hydrogels
such
gelatin,
alginate,
hyaluronic
acid
satisfy
biocompatibility
requirements,
they
distinctly
lack
reproducibility
in
terms
of
mechanical
properties
3D
printability.
Aiming
to
offer
high-performance
alternative,
here
we
present
range
amphiphilic
star-shaped
diblock
copolypeptides
l-glutamate
l-leucine
residues
with
different
topologies.
Hydrophobic
side
chains
the
polymer
block
drive
conformational
self-assembly
water,
spontaneously
forming
tunable
properties,
through
variation
star
topology.
Their
amenable
shear-thinning
self-recovery
render
them
suitable
hydrogel
inks
direct
ink
writing.
Well-defined
3D-printed
structures
can
be
readily
generated
rapidly
photo-cross-linked
using
visible
light
(405
nm)
after
methacrylamide
functionalization,
while
demonstrate
good
top-seeded
encapsulated
MC3T3
cells.
Ice
formation
poses
a
significant
challenge
across
various
fields,
from
industrial
processes
to
biological
preservation.
Developing
antifreeze
agents
and
recognizing
the
mechanism
have
gained
considerable
attention.
Herein,
series
of
poly(l-methionine)
derivatives,
poly(S-carboxymethyl-l-methionine
sulfonium)
(PMetA),
poly(S-methyl-l-methionine
sulfonium
chloride)
(PMetM),
poly(S-carbamidomethyl-l-methionine
(PMetAM),
with
carboxyl,
methyl,
acetamide
groups,
respectively,
are
synthesized
investigated
for
antifreeze.
The
relationship
between
polymer
structure
ice
recrystallization
inhibition
(IRI)
activity
is
examined,
suggesting
that
zwitterionic
PMetA
shows
highest
IRI
activity,
about
27.0
±
3.9%
at
10
mg
mL–1
relative
water.
Results
low-field
nuclear
magnetic
resonance
differential
scanning
calorimetry
indicate
associated
activation
energy
hydrogen
bond
breakage.
exhibits
acceptable
cytocompatibility
10.0
good
cryoprotective
efficiency.
This
finding
provides
valuable
insight
into
mechanism,
contributing
development
potent
cryoprotectants.
Biomacromolecules,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 20, 2025
Poly(l-glutamic
acid)s
(PLGs)
are
promising
bone-targeting
ligands
due
to
their
high
molecular
weight
and
facile
preparation.
Nevertheless,
the
efficiency
of
PLGs
is
still
relatively
low,
validating
necessity
further
enhance
targeting
through
structural
optimization.
Herein,
we
report
use
a
heteropolypeptide
strategy
improve
bone
incorporation
another
side-chain
functionality
for
enhanced
affinity
with
tissues.
Specifically,
introduction
cationic
amino
or
aromatic
phenolic
residues
resulted
in
∼2.3-fold
∼1.6-fold
increase
vivo
targeting,
respectively.
Cationic
modification
not
only
improved
minerals
but
also
exhibited
prolonged
retention
tissues
more
than
60
days.
This
work
highlights
library
screen
optimize
performance
polypeptide
materials,
offering
polymeric
materials
design
bone-related
nanomedicine.
ACS Macro Letters,
Год журнала:
2025,
Номер
14(3), С. 299 - 305
Опубликована: Фев. 21, 2025
Bioadhesives
have
emerged
as
versatile
and
powerful
tools
for
tissue
repair
integration
with
biomedical
devices,
offering
a
wide
range
of
applications
that
captured
significant
clinical
scientific
interest.
Synthetic
polypeptide
adhesives
are
particularly
promising
candidates
bioadhesives,
but
often
face
limitations
in
adhesive
strength.
In
this
study,
inspired
by
marine
proteins,
the
secondary
structure
hydrophobic–hydrophilic
balance
polypeptides
were
precisely
regulated
to
transform
polyelectrolyte
strong
adhesive.
The
resulting
demonstrated
an
strength
exceeding
1.0
MPa,
more
than
10×
higher
previously
reported
synthetic
cohesion
adhesion
can
be
optimized
adjusting
content
hydrophobic
residue
ratios.
More
helices
enhance
interactions
between
backbone
side
chains
well
substrates.
addition,
these
exhibit
excellent
tolerance
acids
or
alkalis,
remarkable
variable
materials
tissues,
impressive
sealing
performance.
Biomacromolecules,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 25, 2025
In
this
study,
we
have
screened
out
an
effective
triphenylphosphine-derived
initiator
(2-TMOPP)
for
efficient
ring-opening
polymerization
of
α-amino
acid
N-carboxyanhydrides
(NCA
ROP)
and
demonstrated
the
potential
prepared
helical
polypeptide
as
ion
channel.
By
optimizing
conditions,
2-TMOPP
exhibited
precise
control
over
molecular
weight
polydispersity
index
polypeptides,
universality
different
NCA
monomers,
ability
to
form
α-helical
secondary
structures.
further
incorporating
binding
groups
regulating
length,
PLCE
was
capable
inserting
into
lipid
bilayers
possessing
function
transport
(H+/K+
antiport)
via
a
channel
mechanism
(EC50
=
12.75
±
1.58
μg
mL-1).
also
showed
anticancer
activity
toward
HeLa
cells,
with
IC50
value
approximately
69.67
1.20
mL-1
after
20
h
coculture,
showing
possibility
future
practical
application
in
biomedical
fields.
Biomacromolecules,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 14, 2025
Controlling
ice
growth
is
crucial
during
the
cryopreservation
of
cells,
but
current
application
small
molecules
as
cryoprotectants
still
remains
a
challenge.
Inspired
by
structures
natural
antifreeze
(glyco)proteins,
in
this
work,
functionalized
poly(l-methionine)s
(PMets)
are
synthesized
with
different
side
groups
including
hydroxyl,
threonine-mimetic
both
methyl
and
hydroxyl
(PMet-MOH),
zwitterion
carboxyl
sulfonium
(PMet-COOH),
glycerol,
trehalose
pendants.
Results
suggest
that
these
PMets
tend
to
self-assemble
into
100-300
nm
nanoparticles
positive
charges
water.
The
functional
have
remarkable
influence
on
their
control
properties.
It
supposed
PMet-MOH
inhibits
possibly
through
adsorption
mechanism
adjacent
groups,
whereas
trehalose-tethered
PMet
can
restrict
diffusion
water
strongest
recrystallization
inhibition
activity
zwitterionic
PMet-COOH
promotes
nucleation
obviously.
This
work
offers
valuable
insight
development
polypeptides
promising
biocompatible
cryoprotectants.
