At the crossroads of biology and electronics

Current Opinion in Biotechnology, Год журнала: 2025, Номер 91, С. 103249 - 103249

Опубликована: Янв. 9, 2025

Язык: Английский

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A Supramolecular Fluorescent Chemosensor Enabling Specific and Rapid Quantification of Norepinephrine Dynamics

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Янв. 30, 2025

Host-guest supramolecular fluorescence probes have garnered significant attention in the detection and sensing of bioactive molecules due to their functionalization potential, adjustable physical properties, high specificity. However, such that reliably, rapidly, specifically measure neurotransmitter dynamics at cellular vivo level yet be reported. Herein, we present a fluorescent chemosensor designed for norepinephrine (NE) detection, showing an exceptional response specificity through host-guest complexation. Multiple covalent/noncovalent interactions, molecular-folding, confinement effect system synergistically enhance selectivity accelerate reaction kinetics down 190 ms. Our enables real-time quantification imaging NE across various models including neuronal cytomembranes, brain tissues, zebrafish. Notably, successfully monitored levels 26 regions freely moving mice under fear-induced stress, revealing elevated concentrations these associated with emotional processing. Thus, our is robust tool measuring diverse model organisms.

Язык: Английский

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Chemiluminescence Resonance Energy Transfer for Targeted Photoactivation of Ion Channels in Living Cells

Angewandte Chemie International Edition, Год журнала: 2025, Номер unknown

Опубликована: Янв. 31, 2025

The regulation of oxidative stress in living cells is essential for maintaining cellular processes and signal transduction. However, developing straightforward strategies to activate stress-sensitive membrane channels situ poses significant challenges. In this study, we present a chemiluminescence resonance energy transfer (CRET) system based on conjugated oligomer, oligo(p-phenylenevinylene)-imidazolium (OPV-Im), designed the activation transient receptor potential melastatin 2 (TRPM2) calcium by superoxide anion (O2⋅-) without requiring external light sources. OPV-Im oligomer targeted cell efficiently, leading TRPM2 CRET process subsequent intracellular overload. This cascade resulted mitochondrial damage inhibition autophagy, ultimately inducing apoptosis. Additionally, strategy could be applied selective killing tumor that overexpress ion inhibiting growth three-dimensional (3D) spheroids. Our offers novel approach regulating channel activity compared optogenetics photodynamic therapy.

Язык: Английский

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Chemiluminescence Resonance Energy Transfer for Targeted Photoactivation of Ion Channels in Living Cells

Angewandte Chemie, Год журнала: 2025, Номер unknown

Опубликована: Янв. 31, 2025

Abstract The regulation of oxidative stress in living cells is essential for maintaining cellular processes and signal transduction. However, developing straightforward strategies to activate stress‐sensitive membrane channels situ poses significant challenges. In this study, we present a chemiluminescence resonance energy transfer (CRET) system based on conjugated oligomer, oligo( p ‐phenylenevinylene)‐imidazolium (OPV−Im), designed the activation transient receptor potential melastatin 2 (TRPM2) calcium by superoxide anion (O ⋅ − ) without requiring external light sources. OPV−Im oligomer targeted cell efficiently, leading TRPM2 CRET process subsequent intracellular overload. This cascade resulted mitochondrial damage inhibition autophagy, ultimately inducing apoptosis. Additionally, strategy could be applied selective killing tumor that overexpress ion inhibiting growth three‐dimensional (3D) spheroids. Our offers novel approach regulating channel activity compared optogenetics photodynamic therapy.

Язык: Английский

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Chemically engineered exogenous organic reactions in living cells for in situ fluorescence imaging and biomedical applications

Journal of Materials Chemistry B, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Key intracellular in situ synthesis processes, including the of near-infrared fluorescent dyes, oxidative cross-linking, polymerization, and bioorthogonal reactions, as well their biomedical applications were summarized.

Язык: Английский

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