3D Tumor-Mimicking Phantom Models for Assessing NIR I/II Nanoparticles in Fluorescence-Guided Surgical Interventions

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

ABSTRACT Fluorescence image-guided surgery (FIGS) offers high spatial resolution and real-time feedback but is limited by shallow tissue penetration autofluorescence from current clinically approved fluorophores. The near-infrared (NIR) spectrum, specifically the NIR-I (700-900 nm) NIR-II (950-1700 nm), addresses these limitations with deeper improved signal-to-noise ratios. However, biological barriers suboptimal optical performance under surgical conditions have hindered clinical translation of NIR-I/II nanoprobes. In vivo mouse models shown promise, do not replicate complex scenarios encountered during real-world surgeries. Existing tissue-mimicking phantoms used to evaluate imaging systems are useful fall short when assessing nanoprobes in environments. These often fail tumor microenvironment, limiting their predictive assessment. To overcome challenges, we propose developing tumor-mimicking phantom (TMPs) that integrate key features, such as tunable cell densities, -like nanoparticle concentrations, biologically relevant factors (pH, enzymes), light absorption components (hemoglobin), scattering (intralipid). TMPs enable more assessments nanoprobes, including profiling, margin delineation, ex thoracic on porcine lungs. can be further modulated closely match profiles tumors. Additionally, 3D bioprinting technology facilitates a high-throughput platform for screening realistic conditions. This approach will identify high-performing probes superior utility, bridging gap between preclinical findings applications, ensuring results extend beyond traditional studies. TOC

Язык: Английский

Characterization of Chiral Thiophene Multilayer 3D Polymers with AIE Properties for Environmental Monitoring of Chromium Ions

Macromolecular Rapid Communications, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Abstract This study reports the synthesis and characterization of novel thiophene‐based multilayer 3D chiral polymers incorporating methoxy octyloxy functional groups. These exhibit unique fluorescence properties, including a pronounced aggregation‐induced emission (AIE) phenomenon at lower excitation wavelength than previously reported thiophene systems. Additionally, demonstrate remarkable sensitivity in detecting hexavalent chromium ions (Cr 6+ ), significant environmental pollutant. The interaction between Cr leads to measurable changes fluorescence, highlighting their potential for applications monitoring biosensing. Importantly, these are effective actual water, where they maintain selectivity despite presence competing metal ions. enhanced further underscores suitability real‐world applications. work contributes field emphasizes versatility advanced sensing

Язык: Английский

Side-Chain Engineering of NIR-II-Emissive Aggregation-Induced Emission Luminogens to Boost Photodynamic and Photothermal Antimicrobial Therapy

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Апрель 18, 2025

The development of antibiotic resistance has made multidrug-resistant bacterial and fungal infections one the most serious health problems worldwide. Photothermal therapy (PTT) photodynamic (PDT) have received increasing attention in antimicrobial fields due to their precision treatment less susceptibility inducing resistance. In particular, developing second near-infrared (NIR-II, 1000-1700 nm)-emissive semiconducting polymers for phototheranostics is highly desirable but remains challenging lack rational molecular design guidelines. Herein, a precise side-chain engineering strategy based on donor-acceptor (D-A)-type semiconductor developed phototherapy. By subtle regulation flexibility, series NIR-II-emissive polymer aggregation-induced-emission (AIE) luminogens (AIEgens) are constructed. optimal PIDT(He)TBT bearing flexible side chains shows physicochemical properties, including highest mass extinction coefficient, best AIE property, red-shifted absorption/emission spectra, photothermal effects. then encapsulated into nanoparticles endow them with water solubility, excellent photostability, enhanced type-I performance terms fluorescence-guided PDT PTT been demonstrated both vitro vivo. This work brings useful insights designing AIEgens efficient phototheranostics.

Язык: Английский