Development and Characterization of a Fluorinated MS-Cleavable Cross-Linker for Structural Proteomics
Journal of the American Society for Mass Spectrometry,
Год журнала:
2025,
Номер
36(6), С. 1410 - 1413
Опубликована: Июнь 4, 2025
Cross-linking
mass
spectrometry
(XL-MS)
is
an
important
method
for
studying
three-dimensional
protein
structures
and
mapping
protein-protein
interactions.
Some
limitations
of
XL-MS
still
consist
its
use
in-cell
in
vivo
applications.
To
date,
cross-linking
reagents
are
urgently
needed
that
can
efficiently
penetrate
the
cell
membrane
to
comprehensively
map
interaction
networks
intact
cells.
In
this
study,
fluorinated
MS-cleavable
cross-linker
bis(pentafluorophenyl)
ureido-4,4'-dibutyrate
(DPFU)
described.
DPFU
based
on
disuccinimidyl
dibutyric
urea
(DSBU)
with
aim
balancing
hydrophobicity
solubility
improve
permeability.
was
evaluated
behavior
different
detergent
solutions
optimize
conditions
potential
application
live
Using
bovine
serum
albumin
(BSA)
as
a
model
protein,
experiments
were
conducted
across
temperatures
concentrations.
Solubility
assays
identified
sodium
dodecyl
sulfate
(SDS)
effective
enhancing
aqueous
environment.
yielded
fewer
cross-links
BSA
than
DSBU,
highlighting
regarding
efficiency
under
similar
experimental
conditions.
This
study
provides
first
insights
into
cross-linkers,
suggesting
further
optimization
broader
future
studies.
Язык: Английский
Improved deconvolution of natural products’ protein targets using diagnostic ions from chemical proteomics linkers
Beilstein Journal of Organic Chemistry,
Год журнала:
2024,
Номер
20, С. 2323 - 2341
Опубликована: Сен. 12, 2024
Identification
of
interactions
between
proteins
and
natural
products
or
similar
active
small
molecules
is
crucial
for
understanding
their
mechanism
action
on
a
molecular
level.
To
search
elusive,
often
labile,
low-abundant
conjugates
compounds,
chemical
proteomics
introduces
feasible
strategy
that
allows
to
enrich
detect
these
conjugates.
Recent
advances
in
mass
spectrometry
techniques
algorithms
provide
unprecedented
depth
proteome
coverage
the
possibility
desired
modified
peptides
with
high
sensitivity.
The
'linker'
connecting
an
compound-protein
conjugate
detection
tag
critical
component
all
proteomic
workflows.
In
this
review,
we
discuss
properties
applications
different
linkers
special
focus
fragmentation
releasing
diagnostic
ions
how
may
improve
confidence
identified
compound-peptide
application
advanced
options
improves
identification
rates
help
identify
otherwise
difficult
find
compounds
proteins,
which
result
from
unperturbed
conditions,
thus
are
physiological
relevance.
Язык: Английский