A dual-switch fluorescence biosensor with entropy-driven and DNA walker cascade amplification circuit for sensitive microRNA detection
Nanoscale,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
To
achieve
precise
diagnosis
of
tumor
cells,
designing
nucleic
acid
amplification
circuits
with
intelligent
multi-switch
responsiveness
for
the
specific
and
sensitive
detection
miRNA
within
cells
is
an
important
strategy.
Here,
we
developed
a
dual-switch
fluorescence
biosensor
that
integrates
two-step
cascade
signal
circuit
entropy-driven
(EDC)
DNA
walkers
onto
gold
nanoparticles
highly
quantitative
target
in
cells.
The
dual
switches
trigger
are
APE1
enzyme,
both
which
upregulated
be
specific,
miRNA21
triggers
upstream
EDC,
releasing
strands
serve
as
downstream
circuit.
Under
cleavage-driven
action
walker
walk
along
track
on
surface
AuNPs,
strong
presenting
good
linearity
concentration
range
40
pM-100
nM.
This
presents
specificity,
anti-interference
ability
against
multiple
RNAs
enzymes
can
effectively
distinguish
cancer
from
normal
cell
lysates.
Overall,
this
provides
recognition
efficient
strategy
tumors,
indicating
its
potential
clinical
applications
disease
diagnosis.
Язык: Английский
Target-Responsive Conformation Switch of Bihairpin Molecular Beacon to Illustrate Ratiometric Fluorescence Biosensing of Dual-Emissive Silver Nanoclusters
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 28, 2025
It
might
be
intriguing
to
explore
the
tailored
ability
of
a
bihairpin-structured
molecular
beacon
(bhMB)
for
improving
reaction
kinetics
and
assay
sensitivity
label-free
ratiometric
fluorescence
specific
trigger
(T*),
in
which
red
green
silver
nanoclusters
(rSNC
gSNC)
were
utilized
as
two
reverse
emitting
reporters.
Unlike
traditional
dually
labeled
MB,
herein
proposed
bhMB
was
designed
with
hairpin
subunits
recognition
signaling
readout.
Four
functional
modules
included:
complement
T*
identifiable
hairpin,
template
sequence
rSNC
gSNC
variable
consensus
loops
capable
complementarily
hybridizing
form
one
paired
stem,
stems
able
merge
into
new
loop.
Upon
introducing
T*,
conformation
switch
triggered
via
affinity
linking
cognate
sequence,
thereby
reconstructing
perfect
stem–loop
monohairpin
structure.
As
such,
mutual
transition
between
nonbinding
binding
states
powered
by
population-shift
mechanism
shift
thermodynamic
equilibrium
toward
state.
Benefiting
from
structure
confinement
bhMB,
local
concentration
reactive
species
increased
speed
up
kinetics.
In
response
route,
clustering
merged
fluorescence,
while
emission
decreased,
pointing
obtained
sensitive
signal
built-in
correction.
Thus,
this
strategy
would
first
example
integrate
recognizing
double-hairpin
biosensing
potential
applications.
Язык: Английский
DNA-Templated Fluorescent Metal Nanoclusters and Their Illuminating Applications
Nanoscale,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
After
the
discovery
of
DNA
during
mid-20
th
century,
a
multitude
novel
methodologies
have
surfaced
which
exploit
for
its
various
properties.
Язык: Английский
Empowering DNA-Based Information Processing: Computation and Data Storage
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 8, 2024
Information
processing
is
a
critical
topic
in
the
digital
age,
as
silicon-based
circuits
face
unprecedented
challenges
such
data
explosion,
immense
energy
consumption,
and
approaching
physical
limits.
Deoxyribonucleic
acid
(DNA),
naturally
selected
carrier
for
storing
using
genetic
information,
possesses
unique
advantages
information
processing,
which
has
given
rise
to
emerging
fields
of
DNA
computing
storage.
To
meet
growing
practical
demands,
wide
variety
materials
interfaces
have
been
introduced
into
technologies,
leading
significant
advancements.
This
review
summarizes
advances
that
facilitate
computation
We
begin
with
brief
overview
fundamental
functions
principles
Subsequently,
we
delve
systems
based
on
various
interfaces,
including
microbeads,
nanomaterials,
nanostructures,
hydrophilic–hydrophobic
compartmentalization,
hydrogels,
metal–organic
frameworks,
microfluidics.
also
explore
storage
systems,
encompassing
encapsulation
materials,
microfluidics
techniques,
living
cells.
Finally,
discuss
current
bottlenecks
obstacles
provide
insights
potential
future
developments.
Язык: Английский