Surface Engineering of MXene and Functional Fullerenols for Cancer Biomarker ‘eIF3d’ DOI Creative Commons
Dilek Söyler, Volkan Dolgun, Oyku Cetin

и другие.

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Selective and sensitive detection of eIF3d (eukaryotic translation initiation factor 3 complex, subunit D), a protein biomarker, is fundamental significance for the diagnosis various cancers. Here, we report an electrochemical sensor based on MXene aspartic acid-functionalized fullerenol (F-Asp) biosensing eIF3d. To construct such innovative sensing platform, was first synthesized, followed by convenient functionalization with acid groups through hydroxylation activation fullerenes. Finally, bioplatform created modifying graphite electrode (GE) surface F-Asp, anti-eIF3d antibody via EDC/NHS chemistry. Detailed analytical material characterization methods were utilized after each modification step. Notably, surface-engineered MXene:F-Asp showed superior features. The sensor's response to achieved in linear range 10 250 ng/mL, limit 0.14 ng/mL. selectivity assessed monitoring its presence variety interfering compounds. Analysis effectively performed synthetic serum samples. promising properties designed suggest great potential real-time health applications.

Язык: Английский

Tracking Globally 5-Methylcytosine and Its Oxidized Derivatives in Colorectal Cancer Epigenome Using Bioelectroanalytical Technologies DOI

Eloy Povedano,

Víctor Pérez‐Ginés, Rebeca M. Torrente‐Rodríguez

и другие.

ACS Sensors, Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

This work presents the first electroanalytical bioplatforms to track individually or simultaneously at a global level all four methylation marks involved in DNA methylation-demethylation cycle: 5-methylcytosine (5mC) and their sequential oxidative derivatives (5-hydroxymethyl-(5hmC), 5-formyl-(5fC), 5-carboxyl-(5caC) cytosines). The employed direct competitive immunoassay formats implemented on surface of magnetic microparticles (MBs) capture antibodies specific each epimark as well synthetic biotinylated oligomers with single that were enzymatically marked horseradish peroxidase (HRP) perform an amperometric readout disposable platforms for multiplexed detection. These new biotechnologies, groundbreaking from analytical clinical perspectives, achieved attractive operational characteristics, reaching detection limits pM levels epimark-bearing oligomers. developed methodology was applied globally target epimarks fast, simple, sensitive, selective way while correlation genomic extracted paired healthy tumor tissues patients colorectal cancer (CRC) established time.

Язык: Английский

Процитировано

0
Surface Engineering of MXene and Functional Fullerenols for Cancer Biomarker ‘eIF3d’ DOI Creative Commons
Dilek Söyler, Volkan Dolgun, Oyku Cetin

и другие.

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Selective and sensitive detection of eIF3d (eukaryotic translation initiation factor 3 complex, subunit D), a protein biomarker, is fundamental significance for the diagnosis various cancers. Here, we report an electrochemical sensor based on MXene aspartic acid-functionalized fullerenol (F-Asp) biosensing eIF3d. To construct such innovative sensing platform, was first synthesized, followed by convenient functionalization with acid groups through hydroxylation activation fullerenes. Finally, bioplatform created modifying graphite electrode (GE) surface F-Asp, anti-eIF3d antibody via EDC/NHS chemistry. Detailed analytical material characterization methods were utilized after each modification step. Notably, surface-engineered MXene:F-Asp showed superior features. The sensor's response to achieved in linear range 10 250 ng/mL, limit 0.14 ng/mL. selectivity assessed monitoring its presence variety interfering compounds. Analysis effectively performed synthetic serum samples. promising properties designed suggest great potential real-time health applications.

Язык: Английский

Процитировано

0