Cytokine & Growth Factor Reviews,
Год журнала:
2022,
Номер
65, С. 1 - 11
Опубликована: Май 2, 2022
Circular
RNA
(circRNA)
is
a
new
endogenous
transcription
product,
which
has
attracted
significant
attention
in
biology
research.CircRNA
comprise
exons
or
introns
involved
regulation
of
various
mechanisms.These
molecules
are
stable
and
species-specific,
as
well
cell
tissue-specific.Cardiovascular
diseases
particularly
myocardial
ischemia
ageing-related
diseases,
pose
major
health
care
burden
novel
treatments
biomarkers
should
be
explored.Recent
findings
indicate
that
circRNAs
implicated
biological
processes,
such
glucose
metabolism,
fatty
acid
oxidation,
mitochondrial
biosynthesis,
implying
they
potential
targets
for
treatment.In
the
present
review,
functions
heart
described,
with
emphasis
given
on
relationship
cardiac
aging-related
diseases.Directions
future
research
also
summarized.
Cardiovascular Research,
Год журнала:
2019,
Номер
unknown
Опубликована: Авг. 20, 2019
The
combination
of
next-generation
sequencing,
advanced
bioinformatics
analysis,
and
molecular
research
has
now
established
circular
RNAs
(circRNAs)
as
a
heterogeneous
group
non-coding
RNA
that
is
widely
abundantly
expressed.
CircRNAs
are
single-stranded
RNA,
covalently
backspliced
to
form
closed
loops.
Different
models
back-splicing
have
been
proposed,
mechanisms
for
circRNA
function
include
sequestering
microRNAs,
direct
interaction
with
proteins,
regulation
transcription,
translation.
Exploring
the
role
circRNAs
in
different
disease
settings,
understanding
how
they
contribute
progression
promises
provide
valuable
insight
into
potential
novel
therapeutic
approaches.
Here,
we
review
growing
number
published
on
heart
cardiovascular
system
summarize
implicated
disease.
Journal of Cellular and Molecular Medicine,
Год журнала:
2021,
Номер
25(16), С. 8028 - 8038
Опубликована: Июнь 25, 2021
Atherosclerosis
can
result
in
multiple
cardiovascular
diseases.
Circular
RNAs
(CircRNAs)
have
been
reported
as
significant
non-coding
atherosclerosis
progression.
Dysfunction
of
vascular
smooth
muscle
cells
(VSMCs)
is
involved
atherosclerosis.
However,
up
to
now,
the
effect
circ_0002984
still
unknown.
Currently,
we
aimed
investigate
function
VSMCs
incubated
by
oxidized
low-density
lipoprotein
(ox-LDL).
Firstly,
our
findings
indicated
that
expression
levels
were
significantly
up-regulated
serum
patients
and
ox-LDL-incubated
VSMCs.
Loss
suppressed
VSMC
viability,
cell
cycle
distribution
migration
capacity.
Then,
carried
out
ELISA
assay
determine
TNF-α
IL-6
levels.
The
data
implied
lack
obviously
repressed
ox-LDL-stimulated
inflammation.
Meanwhile,
miR-326-3p,
which
was
predicted
a
target
circ_0002984,
down-regulated
treated
ox-LDL.
Additionally,
after
overexpression
VSMCs,
decrease
miR-326-3p
observed.
Subsequently,
demonstrated
vesicle-associated
membrane
protein
3
(VAMP3).
Therefore,
hypothesized
could
modulate
VAMP3
through
sponging
miR-326-3p.
Furthermore,
confirmed
up-regulation
rescued
overexpressing-mediated
effects
on
VMSC
inhibitor-mediated
functions
reversed
knockdown
VAMP3.
In
conclusion,
mediated
proliferation,
inflammation
modulating
suggested
might
hold
great
promise
therapeutic
strategy
for
Analytical Chemistry,
Год журнала:
2022,
Номер
94(25), С. 9193 - 9200
Опубликована: Июнь 15, 2022
Circular
ribonucleic
acids
(circRNAs)
are
a
type
of
RNA
that
originates
through
back-splicing
events
from
linear
primary
transcripts.
CircRNAs
display
high
structural
resistance
and
tissue
specificity.
Accurate
quantification
the
circRNA
expression
level
is
vital
importance
to
disease
diagnosis.
Herein,
we
construct
label-free
fluorescent
biosensor
for
ultrasensitive
analysis
circRNAs
based
on
integration
target-initiated
cascade
signal
amplification
strategy
with
light-up
G-quadruplex.
This
assay
involves
only
one
assistant
probe
targets
circRNA-specific
back-splice
junction.
When
present,
it
hybridizes
initiate
duplex-specific
nuclease
(DSN)-catalyzed
cyclic
cleavage
reaction,
producing
abundant
triggers
3′OH
termini.
Then,
terminal
deoxynucleotidyl
transferase
(TdT)
catalyzes
addition
dGTP
dATP
at
3′-OH
termini
resultant
obtain
long
G-rich
DNA
sequences
can
form
efficient
G-quadruplex
products.
The
Thioflavin
T
(ThT)
light
up
G-quadruplex,
generating
an
enhanced
fluorescence.
may
be
performed
isothermally
without
involvement
any
nucleic
acid
templates,
exogenous
primers,
specific
labeled
probes.
Importantly,
this
discriminate
target
one-base
mismatched
exhibits
good
performance
in
human
serum.
Moreover,
accurately
detect
cancer
cells
single-cell
even
differentiate
levels
tissues
healthy
persons
nonsmall
cell
lung
(NSCLC)
patients,
promising
applications
circRNA-related
diagnosis
therapeutics.
Cytokine & Growth Factor Reviews,
Год журнала:
2022,
Номер
65, С. 1 - 11
Опубликована: Май 2, 2022
Circular
RNA
(circRNA)
is
a
new
endogenous
transcription
product,
which
has
attracted
significant
attention
in
biology
research.CircRNA
comprise
exons
or
introns
involved
regulation
of
various
mechanisms.These
molecules
are
stable
and
species-specific,
as
well
cell
tissue-specific.Cardiovascular
diseases
particularly
myocardial
ischemia
ageing-related
diseases,
pose
major
health
care
burden
novel
treatments
biomarkers
should
be
explored.Recent
findings
indicate
that
circRNAs
implicated
biological
processes,
such
glucose
metabolism,
fatty
acid
oxidation,
mitochondrial
biosynthesis,
implying
they
potential
targets
for
treatment.In
the
present
review,
functions
heart
described,
with
emphasis
given
on
relationship
cardiac
aging-related
diseases.Directions
future
research
also
summarized.
