Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018

Cell Death and Differentiation, Год журнала: 2018, Номер 25(3), С. 486 - 541

Опубликована: Янв. 23, 2018

Over the past decade, Nomenclature Committee on Cell Death (NCCD) has formulated guidelines for definition and interpretation of cell death from morphological, biochemical, functional perspectives. Since field continues to expand novel mechanisms that orchestrate multiple pathways are unveiled, we propose an updated classification subroutines focusing mechanistic essential (as opposed correlative dispensable) aspects process. As provide molecularly oriented definitions terms including intrinsic apoptosis, extrinsic mitochondrial permeability transition (MPT)-driven necrosis, necroptosis, ferroptosis, pyroptosis, parthanatos, entotic death, NETotic lysosome-dependent autophagy-dependent immunogenic cellular senescence, mitotic catastrophe, discuss utility neologisms refer highly specialized instances these processes. The mission NCCD is a widely accepted nomenclature in support continued development field.

Язык: Английский

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Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)¹

Autophagy, Год журнала: 2021, Номер 17(1), С. 1 - 382

Опубликована: Янв. 2, 2021

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered field. Our knowledge base relevant new technologies also been expanding. Thus, it is important to formulate on a regular basis updated monitoring autophagy different organisms. Despite numerous reviews, there continues be confusion regarding acceptable methods evaluate autophagy, especially multicellular eukaryotes. Here, present investigators select interpret examine related processes, reviewers provide realistic reasonable critiques reports that are focused these processes. These not meant dogmatic rules, because appropriateness any assay largely depends question being asked system used. Moreover, no individual perfect every situation, calling use multiple techniques properly monitor each experimental setting. Finally, several core components machinery implicated distinct autophagic processes (canonical noncanonical autophagy), implying genetic approaches block should rely targeting two or more autophagy-related genes ideally participate steps pathway. Along similar lines, proteins involved regulate other cellular pathways including apoptosis, all them can used as specific marker bona fide responses. critically discuss current assessing information they can, cannot, provide. ultimate goal encourage intellectual technical innovation

Язык: Английский

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Mechanism of interaction between autophagy and apoptosis in cancer

APOPTOSIS, Год журнала: 2021, Номер 26(9-10), С. 512 - 533

Опубликована: Сен. 12, 2021

Язык: Английский

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Engineered hypoxia-responsive albumin nanoparticles mediating mitophagy regulation for cancer therapy

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 11, 2025

Hypoxic tumors present a significant challenge in cancer therapy due to their ability adaptation low-oxygen environments, which supports tumor survival and resistance treatment. Enhanced mitophagy, the selective degradation of mitochondria by autophagy, is crucial mechanism that helps sustain cellular homeostasis hypoxic tumors. In this study, we develop an azocalix[4]arene-modified supramolecular albumin nanoparticle, co-delivers hydroxychloroquine mitochondria-targeting photosensitizer, designed induce cascaded oxidative stress regulating mitophagy for treatment These nanoparticles are hypoxia-responsive release loaded guest molecules cells. The released disrupts process, thereby increasing further weakening Additionally, upon laser irradiation, photosensitizer generates reactive oxygen species independent oxygen, inducing damage activation. dual action simultaneous spatiotemporal activation flux blockade results enhanced autophagic stress, ultimately driving cell death. Our work highlights effectiveness hydroxychloroquine-mediated combined with mitochondria-targeted cascade-amplified against has been recognized as Here, group fabricates nanoparticle codelivering (HCQ) sulfur-substituted methylated nile blue analog, capable via

Язык: Английский

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Mitochondrial damage by α-synuclein causes cell death in human dopaminergic neurons

Cell Death and Disease, Год журнала: 2019, Номер 10(11)

Опубликована: Ноя. 14, 2019

Abstract Evolving concepts on Parkinson’s disease (PD) pathology suggest that α-synuclein (aSYN) promote dopaminergic neuron dysfunction and death through accumulating in the mitochondria. However, consequence of mitochondrial aSYN localisation structure bioenergetic functions neuronal cells are poorly understood. Therefore, we investigated deleterious effects mitochondria-targeted differentiated human neurons comparison with wild-type (WT) overexpression corresponding EGFP (enhanced green fluorescent protein)-expressing controls. Mitochondria-targeted enhanced reactive oxygen species (ROS) formation, reduced ATP levels showed severely disrupted function dendritic neural network, preceding death. Transmission electron microscopy illustrated distorted cristae many fragmented mitochondria response to WT-aSYN overexpression, a complete loss massively swollen expressing aSYN. Further, analysis bioenergetics neurons, WT or aSYN, elicited pronounced impairment respiration. In pharmacological compound screening, found pan-caspase inhibitors QVD zVAD-FMK, specific caspase-1 inhibitor significantly prevented aSYN-induced cell addition, caspase preserved network activity overexpressing Overall, our findings indicated therapeutic by inhibition despite aSYN-mediated alterations morphology function.

Язык: Английский

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Foam Cells: One Size Doesn’t Fit All

Trends in Immunology, Год журнала: 2019, Номер 40(12), С. 1163 - 1179

Опубликована: Ноя. 12, 2019

Язык: Английский

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Anastasis: recovery from the brink of cell death

Royal Society Open Science, Год журнала: 2018, Номер 5(9), С. 180442 - 180442

Опубликована: Сен. 1, 2018

Anastasis is a natural cell recovery phenomenon that rescues cells from the brink of death. Programmed death such as apoptosis has been traditionally assumed to be an intrinsically irreversible cascade commits rapid and massive demolition. Interestingly, recent studies have demonstrated dying even at late stages generally considered immutable. Here, we examine evidence for anastasis in cultured animals, review findings illuminating potential mechanisms action, discuss challenges studying explore new strategies uncover function regulation anastasis, identification which wide-ranging physiological, pathological therapeutic implications.

Язык: Английский

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Storage lipid studies in tuberculosis reveal that foam cell biogenesis is disease-specific

PLoS Pathogens, Год журнала: 2018, Номер 14(8), С. e1007223 - e1007223

Опубликована: Авг. 30, 2018

Foam cells are lipid-laden macrophages that contribute to the inflammation and tissue damage associated with many chronic inflammatory disorders. Although foam cell biogenesis has been extensively studied in atherosclerosis, how these form during a infectious disease such as tuberculosis is unknown. Here we report that, unlike cholesterol-laden of tuberculous lung lesions accumulate triglycerides. Consequently, varies underlying disease. In vitro mechanistic studies showed triglyceride accumulation human infected Mycobacterium mediated by TNF receptor signaling through downstream activation caspase cascade mammalian target rapamycin complex 1 (mTORC1). These features distinct from known atherogenic establish new paradigm for non-atherogenic formation. Moreover, they reveal novel targets disease-specific pharmacological interventions against maladaptive macrophage responses.

Язык: Английский

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Till Death Do Us Part: The Marriage of Autophagy and Apoptosis

Oxidative Medicine and Cellular Longevity, Год журнала: 2018, Номер 2018(1)

Опубликована: Янв. 1, 2018

Autophagy is a widely conserved catabolic process that necessary for maintaining cellular homeostasis under normal physiological conditions and driving the cell to switch back this status quo times of starvation, hypoxia, oxidative stress. The potential similarities differences between basal autophagy stimulus‐induced are still largely unknown. Both act by clearing aberrant or unnecessary cytoplasmic material, such as misfolded proteins, supernumerary defective organelles. relationship reactive oxygen species (ROS) complex. Cellular ROS predominantly derived from mitochondria. triggered event, organelles effectively, it lowers thereby restoring homeostasis. However, if cannot be reached, cells can choose regulated death response. Intriguingly, autophagic machines both respond same stresses share key regulatory suggesting pathways intricately connected. Here, intersection apoptosis discussed with particular focus on role plays.

Язык: Английский

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Artesunate induces autophagy dependent apoptosis through upregulating ROS and activating AMPK-mTOR-ULK1 axis in human bladder cancer cells

Chemico-Biological Interactions, Год журнала: 2020, Номер 331, С. 109273 - 109273

Опубликована: Сен. 29, 2020

Язык: Английский

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