ACS Sensors,
Год журнала:
2021,
Номер
6(3), С. 842 - 851
Опубликована: Март 16, 2021
The
biopharmaceutical
market
has
been
rapidly
growing
in
recent
years,
creating
a
highly
competitive
arena
where
R&D
is
critical
to
strike
balance
between
clinical
safety
and
profitability.
Toward
process
optimization,
the
development
adoption
of
new
analytical
technologies
(PAT)
highlight
dynamic
complexity
mammalian/human
cell
culture
processes,
as
well
importance
fine-tuning
modeling
key
metabolites
proteins.
In
this
context,
simple,
rapid,
cost-effective
devices
allowing
routine
at-line
monitoring
specific
proteins
during
production
are
currently
lacking.
Here,
we
report
versatile
microfluidic
protein
analysis
cartridge
multiplexed
bead-based
immunodetection
directly
from
complex
mixtures
with
minimal
hands-on
time.
Colorimetric
quantification
Chinese
hamster
ovary
(CHO)
host
impurities,
monoclonal
antibodies
target
biopharmaceuticals,
lactate
dehydrogenase
marker
viability
was
achieved
limits
detection
1–10
ng/mL
range
times
short
30
min.
device
further
demonstrated
for
Rituximab-producing
CHO
bioreactor
over
course
8
days,
providing
comparable
recoveries
standard
enzyme-linked
immunosorbent
assay
(ELISA)
kits.
high
sensitivity
combined
robustness
matrix
interference
highlights
potential
perform
measurements
spanning
downstream
processing.
Frontiers in Immunology,
Год журнала:
2020,
Номер
11
Опубликована: Авг. 28, 2020
Monoclonal
antibodies
(mAbs)
have
become
one
of
the
most
important
classes
biopharmaceutical
products,
and
they
continue
to
dominate
universe
markets
in
terms
approval
sales.
They
are
profitable
single
product
class,
where
represent
six
top
ten
selling
drugs.
At
beginning
1990s,
an
vitro
antibody
selection
technology
known
as
phage
display
was
developed
by
John
McCafferty
Sir.
Gregory
Winter
that
enabled
discovery
human
for
diverse
applications,
particularly
antibody-based
created
combinatorial
libraries
on
filamentous
be
utilized
generating
antigen
specific
a
matter
weeks.
Since
then,
more
than
70
phage–derived
entered
clinical
studies
14
them
been
approved.
These
indicated
cancer,
non-cancer
medical
conditions,
such
inflammatory,
optical,
infectious,
or
immunological
diseases.
Antibody
has
proven
powerful,
versatile
platform
development.
This
review
will
illustrate
utility
powerful
therapeutic
describe
detail
all
approved
mAbs
derived
from
display.
Giant,
Год журнала:
2020,
Номер
3, С. 100022 - 100022
Опубликована: Авг. 1, 2020
Natural
polyphenols
have
attracted
great
interests
in
medicine,
food
and
cosmetics
due
to
their
versatile
functions
such
as
antioxidant,
anticancer
antibacterial.
The
polyphenolic
structures
(i.e.
catechol,
pyrogallol)
natural
are
responsible
for
strong
noncovalent
interactions
via
multiple
hydrogen
bonding
hydrophobic
interactions,
dynamic
covalent
complexation
with
boronate
groups
metal
ions.
In
this
review,
we
focused
on
the
preparation
applications
of
polyphenol-based
coating
films,
nanoparticles,
nanocapsules,
hydrogels
emerged
from
chemical
functional
signatures.
beneficial
role
mechanism
facilitating
delivery
proteins,
nucleic
acids
conventional
drug
molecules
were
reviewed.
Finally,
challenges
systems
perspectives
rational
design
next
generation
polyphenol
biomaterials
will
be
discussed.
Journal of Separation Science,
Год журнала:
2020,
Номер
44(1), С. 35 - 62
Опубликована: Сен. 22, 2020
Fc-Fusion
proteins
represent
a
successful
class
of
biopharmaceutical
products,
with
already
13
drugs
approved
in
the
European
Union
and
United
States
as
well
three
biosimilar
versions
etanercept.
products
combine
tailored
pharmacological
properties
biological
ligands,
together
multiple
functions
fragment
crystallizable
domain
immunoglobulins.
There
is
great
diversity
terms
possible
including
extracellular
domains
natural
receptors,
functionally
active
peptides,
recombinant
enzymes,
genetically
engineered
binding
constructs
acting
cytokine
traps.
Due
to
their
highly
diverse
structures,
analytical
characterization
far
more
complex
than
that
monoclonal
antibodies
requires
use
development
additional
product-specific
methods
over
conventional
generic/platform
methods.
This
can
be
explained,
for
example,
by
presence
numerous
sialic
acids,
leading
high
isoelectric
points
glycosylation
profiles
N-
O-linked
sites.
In
this
review,
we
highlight
wide
range
strategies
used
fully
characterize
Fc-fusion
proteins.
We
also
present
case
studies
on
structural
assessment
all
commercially
available
proteins,
based
features
critical
quality
attributes
ligand-binding
domains.
Molecules,
Год журнала:
2020,
Номер
25(8), С. 1931 - 1931
Опубликована: Апрель 21, 2020
Enantiomeric
separation
is
a
key
step
in
the
development
of
new
chiral
drug.
Preparative
liquid
chromatography
(LC)
continues
to
be
technique
choice
either
during
drug
discovery
process,
achieve
few
milligrams,
or
scale-up
clinical
trial,
needing
kilograms
material.
However,
last
years,
instrumental
and
technical
developments
allowed
an
exponential
increase
preparative
enantioseparation
using
other
techniques.
Besides
LC,
supercritical
fluid
(SFC)
counter-current
(CCC)
have
aroused
interest
for
separation.
This
overview
will
highlight
importance
separations
Medicinal
Chemistry,
especially
early
stages
pipeline
drugs
development.
Few
examples
within
different
methodologies
selected,
emphasizing
trends
The
advantages
drawbacks
critically
discussed.
