Clinical recovery from surgery correlates with single-cell immune signatures

Science Translational Medicine, Год журнала: 2014, Номер 6(255)

Опубликована: Сен. 24, 2014

Single-cell mass cytometry revealed immune correlates of patient-associated variability in surgical recovery.

Язык: Английский

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Scaling by shrinking: empowering single-cell 'omics' with microfluidic devices

Nature Reviews Genetics, Год журнала: 2017, Номер 18(6), С. 345 - 361

Опубликована: Апрель 10, 2017

Язык: Английский

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Combinatorial tetramer staining and mass cytometry analysis facilitate T-cell epitope mapping and characterization

Nature Biotechnology, Год журнала: 2013, Номер 31(7), С. 623 - 629

Опубликована: Июнь 9, 2013

Язык: Английский

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JAK–STAT Pathway Activation in Malignant and Nonmalignant Cells Contributes to MPN Pathogenesis and Therapeutic Response

Cancer Discovery, Год журнала: 2015, Номер 5(3), С. 316 - 331

Опубликована: Янв. 9, 2015

Abstract The identification of JAK2/MPL mutations in patients with myeloproliferative neoplasms (MPN) has led to the clinical development JAK kinase inhibitors, including ruxolitinib. Ruxolitinib reduces splenomegaly and systemic symptoms myelofibrosis improves overall survival; however, mechanism by which inhibitors achieve efficacy not been delineated. Patients MPN present increased levels circulating proinflammatory cytokines, are mitigated inhibitor therapy. We sought elucidate mechanisms attenuate cytokine-mediated pathophysiology. Single-cell profiling demonstrated that hematopoietic cells from models patient samples aberrantly secrete inflammatory cytokines. Pan-hematopoietic Stat3 deletion reduced disease severity attenuated cytokine secretion, similar as observed ruxolitinib In contrast, restricted did reduce or production. Consistent these observations, we found malignant nonmalignant cytokines inhibition production both populations. Significance: Our results demonstrate JAK–STAT3-mediated contributes pathogenesis populations is required for therapeutic efficacy. These findings provide novel insight into achieves MPNs. Cancer Discov; 5(3); 316–31. ©2015 AACR. See related commentary Belver Ferrando, p. 234 This article highlighted Issue feature, 213

Язык: Английский

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The use of skin models in drug development

Advanced Drug Delivery Reviews, Год журнала: 2014, Номер 69-70, С. 81 - 102

Опубликована: Янв. 3, 2014

Язык: Английский

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