A Genetically Encoded Fluorescent Sensor Enables Rapid and Specific Detection of Dopamine in Flies, Fish, and Mice

Cell, Год журнала: 2018, Номер 174(2), С. 481 - 496.e19

Опубликована: Июль 1, 2018

Язык: Английский

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A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine

Neuron, Год журнала: 2019, Номер 102(4), С. 745 - 761.e8

Опубликована: Март 25, 2019

Язык: Английский

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Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks

Chemical Reviews, Год журнала: 2018, Номер 118(24), С. 11707 - 11794

Опубликована: Дек. 14, 2018

Cellular signaling networks are the foundation which determines fate and function of cells as they respond to various cues stimuli. The discovery fluorescent proteins over 25 years ago enabled development a diverse array genetically encodable biosensors that capable measuring spatiotemporal dynamics signal transduction pathways in live cells. In an effort encapsulate breadth have expanded, we endeavored assemble comprehensive list published engineered biosensors, discuss many molecular designs utilized their development. Then, review how high temporal spatial resolution afforded by has aided our understanding regulation at cellular subcellular level. Finally, highlight some emerging areas research both biosensor design applications on forefront

Язык: Английский

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Next-generation GRAB sensors for monitoring dopaminergic activity in vivo

Nature Methods, Год журнала: 2020, Номер 17(11), С. 1156 - 1166

Опубликована: Окт. 21, 2020

Язык: Английский

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An optimized acetylcholine sensor for monitoring in vivo cholinergic activity

Nature Methods, Год журнала: 2020, Номер 17(11), С. 1139 - 1146

Опубликована: Сен. 28, 2020

Язык: Английский

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Improved tools to study astrocytes

Nature reviews. Neuroscience, Год журнала: 2020, Номер 21(3), С. 121 - 138

Опубликована: Фев. 10, 2020

Язык: Английский

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A genetically encoded single-wavelength sensor for imaging cytosolic and cell surface ATP

Nature Communications, Год журнала: 2019, Номер 10(1)

Опубликована: Фев. 12, 2019

Abstract Adenosine 5′ triphosphate (ATP) is a universal intracellular energy source and an evolutionarily ancient, ubiquitous extracellular signal in diverse species. Here, we report the generation characterization of single-wavelength genetically encoded fluorescent sensors (iATPSnFRs) for imaging cytosolic ATP from insertion circularly permuted superfolder GFP into epsilon subunit F 0 1 -ATPase Bacillus PS3 . On cell surface within cytosol, iATPSnFR 1.0 responds to relevant concentrations (30 μM 3 mM) with fast increases fluorescence. iATPSnFRs can be targeted specific types sub-cellular compartments, imaged standard light microscopes, do not respond other nucleotides nucleosides, when fused red protein function as ratiometric indicators. After careful consideration their modest pH sensitivity, represent promising reagents space cells during variety settings, further application-specific refinements.

Язык: Английский

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A genetically encoded sensor for measuring serotonin dynamics

Nature Neuroscience, Год журнала: 2021, Номер 24(5), С. 746 - 752

Опубликована: Апрель 5, 2021

Язык: Английский

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A Motivational and Neuropeptidergic Hub: Anatomical and Functional Diversity within the Nucleus Accumbens Shell

Neuron, Год журнала: 2019, Номер 102(3), С. 529 - 552

Опубликована: Май 1, 2019

Язык: Английский

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Next-generation interfaces for studying neural function

Nature Biotechnology, Год журнала: 2019, Номер 37(9), С. 1013 - 1023

Опубликована: Авг. 12, 2019

Язык: Английский

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