Plant–microbiome interactions: from community assembly to plant health

Nature Reviews Microbiology, Год журнала: 2020, Номер 18(11), С. 607 - 621

Опубликована: Авг. 12, 2020

Язык: Английский

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Root exudate metabolites drive plant-soil feedbacks on growth and defense by shaping the rhizosphere microbiota

Nature Communications, Год журнала: 2018, Номер 9(1)

Опубликована: Июль 10, 2018

Abstract By changing soil properties, plants can modify their growth environment. Although the microbiota is known to play a key role in resulting plant-soil feedbacks, proximal mechanisms underlying this phenomenon remain unknown. We found that benzoxazinoids, class of defensive secondary metabolites are released by roots cereals such as wheat and maize, alter root-associated fungal bacterial communities, decrease plant growth, increase jasmonate signaling defenses, suppress herbivore performance next generation. Complementation experiments demonstrate benzoxazinoid breakdown product 6-methoxy-benzoxazolin-2-one (MBOA), which accumulates during conditioning phase, both sufficient necessary trigger observed phenotypic changes. Sterilization, profiling complementation reveal MBOA acts indirectly altering microbiota. Our results mechanism determine composition rhizosphere microbiota, plant-herbivore interactions

Язык: Английский

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Microbial interactions within the plant holobiont

Microbiome, Год журнала: 2018, Номер 6(1)

Опубликована: Март 27, 2018

Since the colonization of land by ancestral plant lineages 450 million years ago, plants and their associated microbes have been interacting with each other, forming an assemblage species that is often referred to as a "holobiont." Selective pressure acting on holobiont components has likely shaped plant-associated microbial communities selected for host-adapted microorganisms impact fitness. However, high densities detected tissues, together fast generation time more ancient origin compared host, suggest microbe-microbe interactions are also important selective forces sculpting complex assemblages in phyllosphere, rhizosphere, endosphere compartments. Reductionist approaches conducted under laboratory conditions critical decipher strategies used specific cooperate compete within or outside tissues. Nonetheless, our understanding these shaping communities, along relevance host health natural context, remains sparse. Using examples obtained from reductionist community-level approaches, we discuss fundamental role (prokaryotes micro-eukaryotes) community structure health. We provide conceptual framework illustrating among microbiota members establishment maintenance host-microbial homeostasis.

Язык: Английский

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Agricultural intensification reduces microbial network complexity and the abundance of keystone taxa in roots

The ISME Journal, Год журнала: 2019, Номер 13(7), С. 1722 - 1736

Опубликована: Март 8, 2019

Root-associated microbes play a key role in plant performance and productivity, making them important players agroecosystems. So far, very few studies have assessed the impact of different farming systems on root microbiota it is still unclear whether agricultural intensification influences structure complexity microbial communities. We investigated conventional, no-till, organic wheat fungal communities using PacBio SMRT sequencing samples collected from 60 farmlands Switzerland. Organic harbored much more complex network with significantly higher connectivity than conventional no-till systems. The abundance keystone taxa was highest under where lowest. also found strong negative association (R2 = 0.366; P < 0.0001) between connectivity. occurrence best explained by soil phosphorus levels, bulk density, pH, mycorrhizal colonization. majority are known to form arbuscular associations plants belong orders Glomerales, Paraglomerales, Diversisporales. Supporting this, fungi roots soils farming. To our knowledge, this first study report for agroecosystems, we demonstrate that reduces microbiome.

Язык: Английский

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Host genotype and age shape the leaf and root microbiomes of a wild perennial plant

Nature Communications, Год журнала: 2016, Номер 7(1)

Опубликована: Июль 12, 2016

Bacteria living on and in leaves roots influence many aspects of plant health, so the extent a plant's genetic control over its microbiota is great interest to crop breeders evolutionary biologists. Laboratory-based studies, because they poorly simulate true environmental heterogeneity, may misestimate or totally miss certain host genes microbiome. Here we report large-scale field experiment disentangle effects genotype, environment, age year harvest bacterial communities associated with Boechera stricta (Brassicaceae), perennial wild mustard. Host microbiome evident but not roots, varies substantially among sites. Microbiome composition also shifts as plants age. Furthermore, large proportion leaf groups are shared suggesting inoculation from soil. Our results demonstrate how genotype-by-environment interactions contribute complexity assembly natural environments.

Язык: Английский

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Research priorities for harnessing plant microbiomes in sustainable agriculture

PLoS Biology, Год журнала: 2017, Номер 15(3), С. e2001793 - e2001793

Опубликована: Март 28, 2017

Feeding a growing world population amidst climate change requires optimizing the reliability, resource use, and environmental impacts of food production. One way to assist in achieving these goals is integrate beneficial plant microbiomes—i.e., those enhancing growth, nutrient use efficiency, abiotic stress tolerance, disease resistance—into agricultural This integration will require large-scale effort among academic researchers, industry farmers understand manage plant-microbiome interactions context modern systems. Here, we identify priorities for research this area: (1) develop model host–microbiome systems crop plants non-crop with associated microbial culture collections reference genomes, (2) define core microbiomes metagenomes systems, (3) elucidate rules synthetic, functionally programmable microbiome assembly, (4) determine functional mechanisms interactions, (5) characterize refine genotype-by-environment-by-microbiome-by-management interactions. Meeting should accelerate our ability design implement effective manipulations management strategies, which, turn, pay dividends both consumers producers supply.

Язык: Английский

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Understanding and exploiting plant beneficial microbes

Current Opinion in Plant Biology, Год журнала: 2017, Номер 38, С. 155 - 163

Опубликована: Июнь 14, 2017

After a century of incremental research, technological advances, coupled with need for sustainable crop yield increases, have reinvigorated the study beneficial plant-microbe interactions attention focused on how microbiomes alter plant phenotypes. We review recent advances in microbiome and describe potential applications increasing productivity. The phylogenetic diversity is increasingly well characterized, their functional becoming more accessible. Large culture collections are available controlled experimentation, to come. Genetic resources being brought bear questions function. expect that microbial amendments varying complexities will expose rules governing contributing growth promotion disease resistance, enabling agriculture.

Язык: Английский

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Bacterial Endophyte Colonization and Distribution within Plants

Microorganisms, Год журнала: 2017, Номер 5(4), С. 77 - 77

Опубликована: Ноя. 25, 2017

The plant endosphere contains a diverse group of microbial communities. There is general consensus that these communities make significant contributions to health. Both recently adopted genomic approaches and classical microbiology techniques continue develop the science plant-microbe interactions. Endophytes are symbionts residing within for majority their life cycle without any detrimental impact host plant. use natural offers an opportunity maximize crop productivity while reducing environmental impacts agriculture. promote growth through nitrogen fixation, phytohormone production, nutrient acquisition, by conferring tolerance abiotic biotic stresses. Colonization endophytes crucial providing benefits Endophytic colonization refers entry, multiplication endophyte populations Lately, microbiome research has gained considerable attention but mechanism allowing plants recruit largely unknown. This review summarizes currently available knowledge about endophytic bacteria in various species, specifically discusses maize Populus endophytes.

Язык: Английский

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Drought Stress and Root-Associated Bacterial Communities

Frontiers in Plant Science, Год журнала: 2018, Номер 8

Опубликована: Янв. 9, 2018

Root-associated bacterial communities play a vital role in maintaining health of the plant host. These exist complex relationships, where composition and abundance community members is dependent on number factors such as local soil chemistry, genotype phenotype, perturbations surrounding abiotic environment. One common perturbation, drought, has been shown to have drastic effects communities, yet little understood about underlying causes behind observed shifts microbial abundance. As drought may affect root both directly by modulating moisture availability, well indirectly altering chemistry phenotypes, we provide synthesis trends recent studies discuss possible directions for future research that hope will more knowledgeable predictions responses events.

Язык: Английский

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Simplified and representative bacterial community of maize roots

Proceedings of the National Academy of Sciences, Год журнала: 2017, Номер 114(12)

Опубликована: Март 8, 2017

Plant-associated microbes are important for the growth and health of their hosts. As a result numerous prior studies, we know that host genotypes abiotic factors influence composition plant microbiomes. However, high complexity these communities challenges detailed studies to define experimentally mechanisms underlying dynamics community assembly beneficial effects such microbiomes on In this work, from distinctive microbiota assembled by maize roots, through host-mediated selection, obtained greatly simplified synthetic bacterial consisting seven strains (Enterobacter cloacae, Stenotrophomonas maltophilia, Ochrobactrum pituitosum, Herbaspirillum frisingense, Pseudomonas putida, Curtobacterium pusillum, Chryseobacterium indologenes) representing three four most dominant phyla found in roots. By using selective culture-dependent method track abundance each strain, investigated role plays roots axenic seedlings. Only removal E. cloacae led complete loss community, C. pusillum took over. This suggests keystone species model ecosystem. planta vitro, inhibited phytopathogenic fungus Fusarium verticillioides, indicating clear benefit host. Thus, combined with quantification method, our seven-species root microbiome has potential serve as useful system explore how interspecies interactions affect dissect hosts under laboratory conditions future.

Язык: Английский

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