Acquisition of the arginine deiminase system benefits epiparasitic Saccharibacteria and their host bacteria in a mammalian niche environment

Proceedings of the National Academy of Sciences, Год журнала: 2022, Номер 119(2)

Опубликована: Янв. 6, 2022

Significance The Candidate Phyla Radiation (CPR) is a large monophyletic lineage with poorly understood biology. Saccharibacteria are ultrasmall parasitic CPR bacteria highly reduced genomes that have made the transition from an environmental origin to mammals. We tested function and impact of arginine deiminase system (ADS), catabolism pathway likely acquired by mammal-associated during their environment-to-mammal niche transition. showed ADS not only helped facilitate Saccharibacterial adaptation mammals but also contributed establishment cooperative episymbiotic interaction bacterial hosts within mammalian microbiomes. Our study provides experimental evidence demonstrating importance in facilitating environment niche.

Язык: Английский

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Longitudinal transmission of bacterial and fungal communities from seed to seed in rice

Communications Biology, Год журнала: 2022, Номер 5(1)

Опубликована: Авг. 1, 2022

Abstract Vertical transmission of microbes is crucial for the persistence host-associated microbial communities. Although vertical seed has been reported from diverse plants, ecological mechanisms and dynamics communities parent to progeny remain scarce. Here we reveal veiled mechanism governing bacterial fungal in rice across two consecutive seasons. We identify 29 34 members transmitted generations. Abundance-based regression models allow classify colonization types microbes. find that they are late colonizers dominating each community at ripening stage. Ecological further show observed temporal patterns affected by niche change neutrality. Source-sink modeling reveals parental seeds stem endosphere major origins This study gives empirical evidence as an continuum during seed-to-seed transmission.

Язык: Английский

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Recent insights into aquatic viruses: Emerging and reemerging pathogens, molecular features, biological effects, and novel investigative approaches

Water Biology and Security, Год журнала: 2022, Номер 1(4), С. 100062 - 100062

Опубликована: Июль 31, 2022

Aquatic viruses are naturally present in the aquatic environment and number of is staggering. Various multicellular organisms ecosystems may be infected, cross-species transmitted, manipulated, killed by viruses, which can lead to cascading ecological effects. The unicellular alter interactions between host individuals, essential effecting or maintaining dynamics microbial communities, horizontal gene transfer, biodiversity, modulating processes globally. Meanwhile, hosts also impact viral abundance diversity. Microbial diversity drives multifunctionality ecosystems, while shape complex communities crucial for ecosystem functioning. This review focuses on molecular, genetic, evolutionary, ecosystemic advances related emerging reemerging presents contexts, novel tools, investigative approaches pertaining study virology, discusses mechanisms affect ecosystems. paper provides an efficient broadly-based blueprint improving understanding viruses.

Язык: Английский

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Episymbiotic Saccharibacteria TM7x modulates the susceptibility of its host bacteria to phage infection and promotes their coexistence

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(16)

Опубликована: Апрель 9, 2024

Bacteriophages (phages) play critical roles in modulating microbial ecology. Within the human microbiome, factors influencing long-term coexistence of phages and bacteria remain poorly investigated. Saccharibacteria (formerly TM7) are ubiquitous members oral microbiome. These ultrasmall form episymbiotic relationships with their host impact physiology. Here, we showed that during surface-associated growth, a isolate (named TM7x) protects its bacterium, Schaalia odontolytica strain XH001) against lytic phage LC001 predation. RNA-Sequencing analysis identified XH001 gene cluster predicted functions involved biogenesis cell wall polysaccharides (CWP), whose expression is significantly down-regulated when forming symbiosis TM7x. Through genetic work, experimentally demonstrated this CWP on bacterial–phage interaction by affecting binding. In vitro coevolution experiments further heterogeneous populations TM7x-associated TM7x-free XH001, which display differential susceptibility to predation, promote coexistence. Our study highlights tripartite between episymbiont, phage. More importantly, present mechanism, i.e., episymbiont-mediated modulation bacteria, impacts predation contributes formation “source-sink” dynamics biofilm, promoting within

Язык: Английский

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Bacterial lifestyle shapes pangenomes

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(21)

Опубликована: Май 14, 2024

Pangenomes vary across bacteria. Some species have fluid pangenomes, with a high proportion of genes varying between individual genomes. Other less different genomes tending to contain the same genes. Two main hypotheses been suggested explain this variation: differences in species' bacterial lifestyle and effective population size. However, previous studies not able test these because features size are highly correlated each other, phylogenetically conserved, making it hard disentangle their relative importance. We used phylogeny-based analyses, 126 species, tease apart causal role factors. found that pangenome fluidity was lower i) host-associated compared free-living ii) obligately dependent on host, live inside cells, more pathogenic motile. In contrast, we no support for competing hypothesis larger sizes lead pangenomes. Effective appears correlate variation is also driven by lifestyle, rather than relationship.

Язык: Английский

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