The importance of anabolism in microbial control over soil carbon storage

Nature Microbiology, Год журнала: 2017, Номер 2(8)

Опубликована: Июль 24, 2017

Язык: Английский

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Universal molecular structures in natural dissolved organic matter

Nature Communications, Год журнала: 2018, Номер 9(1)

Опубликована: Авг. 3, 2018

Abstract Natural dissolved organic matter (DOM) comprises a broad range of molecules in aquatic systems and is among the most complex molecular mixtures known. Here we show, by comparing detailed structural fingerprints individual formulae DOM from set four marine one freshwater environments, that major component molecularly indistinguishable these diverse samples. Molecular conformity was not only apparent co-occurrence thousands identical formulae, but also features those isomers collectively represent formula. The presence large pool compounds with likely result cascade degradation processes or common synthetic pathways ultimately lead to formation universal background, regardless origin history material. This novel insight impacts our understanding long-term turnover as underlying mechanisms are possibly universal.

Язык: Английский

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Where Carbon Goes When Water Flows: Carbon Cycling across the Aquatic Continuum

Frontiers in Marine Science, Год журнала: 2017, Номер 4

Опубликована: Янв. 31, 2017

The purpose of this review is to highlight progress in unraveling carbon cycling dynamics across the continuum landscapes, inland waters, coastal oceans, and atmosphere. Earth systems are intimately interconnected, yet most biogeochemical studies focus on specific components isolation. movement water drives cycle, and, as such, waters provide a critical intersection between terrestrial marine biospheres. Inland, estuarine, well studied regions near centers human population Northern hemisphere. However, many world's large river their receiving remain poorly characterized, particularly tropics, which contribute disproportionately fraction transformation organic matter dioxide, Arctic, where positive feedback mechanisms likely amplify global climate change. There gaps current coverage environmental observations along aquatic continuum. For example, tidally-influenced reaches major rivers near-shore around plumes often left out budgets due combination methodological constraints poor data coverage. We suggest that closing these could potentially alter estimates CO2 outgassing from surface atmosphere by several-fold. Finally, order identify constrain/embrace uncertainties budget estimations it important we further adopt statistical modeling approaches have become well-established fields oceanography paleoclimatology, for example.

Язык: Английский

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Deciphering ocean carbon in a changing world

Proceedings of the National Academy of Sciences, Год журнала: 2016, Номер 113(12), С. 3143 - 3151

Опубликована: Март 7, 2016

Dissolved organic matter (DOM) in the oceans is one of largest pools reduced carbon on Earth, comparable size to atmospheric CO 2 reservoir. A vast number compounds are present DOM, and they play important roles all major element cycles, contribute storage ocean, support marine ecosystems, facilitate interactions between organisms. At heart DOM cycle lie molecular-level relationships individual members ocean microbiome that produce consume them. In past, these connections have eluded clear definition because sheer numerical complexity both molecules microorganisms. Emerging tools analytical chemistry, microbiology, informatics breaking down barriers a fuller appreciation connections. Here we highlight questions being addressed using recent methodological technological developments those fields consider how advances transforming our understanding some most reactions cycle.

Язык: Английский

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The global ocean microbiome

Science, Год журнала: 2015, Номер 350(6266)

Опубликована: Дек. 11, 2015

The microbiome of the largest environment on Earth has been gradually revealing its secrets over four decades study. Despite dispersed nature substrates and transience surfaces, marine microbes drive essential transformations in all global elemental cycles. Much learned about that carry out key biogeochemical processes, but there are still plenty ambiguities factors important regulating activity, including role microbial interactions. Identifying molecular "currencies" exchanged within community will provide information function vulnerability to environmental change.

Язык: Английский

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Moving beyond the van Krevelen Diagram: A New Stoichiometric Approach for Compound Classification in Organisms

Analytical Chemistry, Год журнала: 2018, Номер 90(10), С. 6152 - 6160

Опубликована: Апрель 19, 2018

van Krevelen diagrams (O/C vs H/C ratios of elemental formulas) have been widely used in studies to obtain an estimation the main compound categories present environmental samples. However, limits defining a specific category based solely on O/C and formulas never accurately listed or proposed classify metabolites biological Furthermore, while can provide overview categories, such classification is inefficient because large overlap among different along both axes. We propose more accurate for samples analyzed by high-resolution mass spectrometry assessment C/H/O/N/P stoichiometric over 130 000 compounds classified 6 categories: lipids, peptides, amino sugars, carbohydrates, nucleotides, phytochemical (oxy-aromatic compounds). Our multidimensional (MSCC) constraints showed highly categorization with 98% accuracy representing substantial improvement any classic diagram. This method represents signficant step forward research, especially ecological stoichiometry eco-metabolomics studies, providing novel robust tool improve our understanding ecosystem structure function through chemical characterization

Язык: Английский

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Dynamic exchanges between DOM and POM pools in coastal and inland aquatic ecosystems: A review

The Science of The Total Environment, Год журнала: 2016, Номер 551-552, С. 415 - 428

Опубликована: Фев. 15, 2016

Язык: Английский

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SAR202 Genomes from the Dark Ocean Predict Pathways for the Oxidation of Recalcitrant Dissolved Organic Matter

mBio, Год журнала: 2017, Номер 8(2)

Опубликована: Апрель 19, 2017

ABSTRACT Deep-ocean regions beyond the reach of sunlight contain an estimated 615 Pg dissolved organic matter (DOM), much which persists for thousands years. It is thought that bacteria oxidize DOM until it too dilute or refractory to support microbial activity. We analyzed five single-amplified genomes (SAGs) from abundant SAR202 clade dark-ocean bacterioplankton and found they encode multiple families paralogous enzymes involved in carbon catabolism, including several oxidative we hypothesize participate degradation cyclic alkanes. The partial encoded 152 flavin mononucleotide/F420-dependent monooxygenases (FMNOs), many are predicted be type II Baeyer-Villiger (BVMOs) catalyze oxygen insertion into semilabile alicyclic large number enzymes, as well other appear play complementary roles catabolic pathways, suggests might final steps biological oxidation relatively recalcitrant compounds persist. IMPORTANCE Carbon ocean massively sequestered a complex mixture biologically molecules accumulate chemical end member diagenetic change. However, few details known about biochemical machinery sequestration deep ocean. Reconstruction metabolism deep-ocean clade, SAR202, led postulation new pathways may penultimate stages forms These tied proliferation enzymes. This research illuminates biochemistry broadly consequential reconstructing global cycle.

Язык: Английский

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Microbial transformation of virus-induced dissolved organic matter from picocyanobacteria: coupling of bacterial diversity and DOM chemodiversity

The ISME Journal, Год журнала: 2019, Номер 13(10), С. 2551 - 2565

Опубликована: Июнь 21, 2019

Язык: Английский

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A new conceptual framework for the transformation of groundwater dissolved organic matter

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Апрель 20, 2022

Groundwater comprises 95% of the liquid fresh water on Earth and contains a diverse mix dissolved organic matter (DOM) molecules which play significant role in global carbon cycle. Currently, storage times degradation pathways groundwater DOM are unclear, preventing an accurate estimate sources sinks for budgets. Here we reveal transformations aging using ultra-high resolution mass spectrometry combined with radiocarbon dating. Long-term anoxia lack photodegradation leads to removal oxidised build-up both reduced photodegradable formulae aerobically biolabile strong microbial signal. This contrasts pathway oxic marine, river, lake systems. Our findings suggest that processes such as extraction subterranean discharge oceans could result up 13 Tg highly photolabile released surface environments per year, where it can be rapidly degraded. These highlight importance considering

Язык: Английский

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