Fluorescence in situ hybridization (FISH): History, limitations and what to expect from micro-scale FISH? DOI Creative Commons

Deborah Huber,

Lena Voith von Voithenberg, Govind V. Kaigala

и другие.

Micro and Nano Engineering, Год журнала: 2018, Номер 1, С. 15 - 24

Опубликована: Окт. 31, 2018

In this article, we review an important cytogenetic technique - fluorescence in situ hybridization (FISH) which is used for obtaining spatial genomic and transcriptomic information. FISH widely utilized cell biological research as well diagnostic applications preventive reproductive medicine, oncology. It the gold standard detection of chromosomal abnormalities. Despite high specificity possibility direct quantitative imaging, some its key limitations prevent regular use diagnostics. To promote extensive these applications, assay time probe consumption will need to be addressed. Microfluidic technologies hold great promise improving exactly parameters. past two decades, microtechnology has matured enabled a new line analysis tools biomedical chemical sciences. Incidentally, convergence with microfluidics starting have decisive impact field medical By miniaturizing implementations assays, special characteristics fluid flow small volumes can leveraged modify reaction kinetics thus reagent delivery assays. Here highlight selected historical views on FISH, current implementations, provide perspective future developments micro-scale FISH.

Язык: Английский

Organization and function of the 3D genome DOI
Boyan Bonev, Giacomo Cavalli

Nature Reviews Genetics, Год журнала: 2016, Номер 17(11), С. 661 - 678

Опубликована: Окт. 14, 2016

Язык: Английский

Процитировано

1039

Organizational principles of 3D genome architecture DOI
M. Jordan Rowley, Victor G. Corces

Nature Reviews Genetics, Год журнала: 2018, Номер 19(12), С. 789 - 800

Опубликована: Окт. 26, 2018

Язык: Английский

Процитировано

1028

High-definition spatial transcriptomics for in situ tissue profiling DOI
Sanja Vicković, Gökçen Eraslan, Fredrik Salmén

и другие.

Nature Methods, Год журнала: 2019, Номер 16(10), С. 987 - 990

Опубликована: Сен. 9, 2019

Язык: Английский

Процитировано

952

Fluorescence nanoscopy in cell biology DOI
Steffen J. Sahl, Stefan W. Hell, Stefan Jakobs

и другие.

Nature Reviews Molecular Cell Biology, Год журнала: 2017, Номер 18(11), С. 685 - 701

Опубликована: Сен. 6, 2017

Язык: Английский

Процитировано

912

Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells DOI Open Access
Bogdan Bintu,

Leslie J. Mateo,

Jun-Han Su

и другие.

Science, Год журнала: 2018, Номер 362(6413)

Опубликована: Окт. 26, 2018

The spatial organization of chromatin is pivotal for regulating genome functions. We report an imaging method tracing with kilobase- and nanometer-scale resolution, unveiling conformation across topologically associating domains (TADs) in thousands individual cells. Our data revealed TAD-like structures globular sharp domain boundaries single varied from cell to cell, occurring nonzero probabilities at all genomic positions but preferentially CCCTC-binding factor (CTCF)- cohesin-binding sites. Notably, cohesin depletion, which abolished TADs the population-average level, did not diminish cells eliminated preferential boundary positions. Moreover, we observed widespread, cooperative, multiway interactions, remained after depletion. These results provide critical insight into mechanisms underlying hub formation.

Язык: Английский

Процитировано

903

Super-resolution imaging reveals distinct chromatin folding for different epigenetic states DOI
Alistair N. Boettiger, Bogdan Bintu, Jeffrey R. Moffitt

и другие.

Nature, Год журнала: 2016, Номер 529(7586), С. 418 - 422

Опубликована: Янв. 1, 2016

Язык: Английский

Процитировано

858

Chromatin Domains: The Unit of Chromosome Organization DOI Creative Commons
Jesse R. Dixon, David U. Gorkin, Bing Ren

и другие.

Molecular Cell, Год журнала: 2016, Номер 62(5), С. 668 - 680

Опубликована: Июнь 1, 2016

Язык: Английский

Процитировано

776

Spatial organization of chromatin domains and compartments in single chromosomes DOI Open Access
Siyuan Wang, Jun-Han Su, Brian J. Beliveau

и другие.

Science, Год журнала: 2016, Номер 353(6299), С. 598 - 602

Опубликована: Июль 22, 2016

Spatial organization inside the nucleus In eukaryotic cells, DNA is packaged into a complex macromolecular structure called chromatin. Wang et al. have developed an imaging method to map position of multiple regions on individual chromosomes, and results confirm that chromatin organized large contact domains TADS (topologically associating domains). Unexpectedly, though, folding deviates from classical fractal-globule model at length scales. Science , this issue p. 598

Язык: Английский

Процитировано

614

Methods for mapping 3D chromosome architecture DOI
Rieke Kempfer, Ana Pombo

Nature Reviews Genetics, Год журнала: 2019, Номер 21(4), С. 207 - 226

Опубликована: Дек. 17, 2019

Язык: Английский

Процитировано

453

Protection of tissue physicochemical properties using polyfunctional crosslinkers DOI

Young-Gyun Park,

Chang Ho Sohn, Ritchie Chen

и другие.

Nature Biotechnology, Год журнала: 2018, Номер 37(1), С. 73 - 83

Опубликована: Дек. 17, 2018

Язык: Английский

Процитировано

391