The history and advances in cancer immunotherapy: understanding the characteristics of tumor-infiltrating immune cells and their therapeutic implications

Cellular and Molecular Immunology, Год журнала: 2020, Номер 17(8), С. 807 - 821

Опубликована: Июль 1, 2020

Abstract Immunotherapy has revolutionized cancer treatment and rejuvenated the field of tumor immunology. Several types immunotherapy, including adoptive cell transfer (ACT) immune checkpoint inhibitors (ICIs), have obtained durable clinical responses, but their efficacies vary, only subsets patients can benefit from them. Immune infiltrates in microenvironment (TME) been shown to play a key role development will affect outcomes patients. Comprehensive profiling tumor-infiltrating cells would shed light on mechanisms cancer–immune evasion, thus providing opportunities for novel therapeutic strategies. However, highly heterogeneous dynamic nature TME impedes precise dissection intratumoral cells. With recent advances single-cell technologies such as RNA sequencing (scRNA-seq) mass cytometry, systematic interrogation is feasible provide insights into functional diversities In this review, we outline progress particularly by focusing landmark studies characterization tumor-associated cells, summarize phenotypic connections with immunotherapy. We believe review could strengthen our understanding facilitate elucidation modulation progression, guide immunotherapies treatment.

Язык: Английский

---

New Technologies for Analysis of Extracellular Vesicles

Chemical Reviews, Год журнала: 2018, Номер 118(4), С. 1917 - 1950

Опубликована: Янв. 31, 2018

Extracellular vesicles (EVs) are diverse, nanoscale membrane actively released by cells. Similar-sized can be further classified (e.g., exosomes, microvesicles) based on their biogenesis, size, and biophysical properties. Although initially thought to cellular debris, thus under-appreciated, EVs now increasingly recognized as important vehicles of intercellular communication circulating biomarkers for disease diagnoses prognosis. Despite clinical potential, the lack sensitive preparatory analytical technologies poses a barrier translation. New platforms including molecular ones being developed address these challenges. Recent advances in field expected have far-reaching impact both basic translational studies. This article aims present comprehensive critical overview emerging EV detection applications.

Язык: Английский

---

Intratumor Heterogeneity: The Rosetta Stone of Therapy Resistance

Cancer Cell, Год журнала: 2020, Номер 37(4), С. 471 - 484

Опубликована: Апрель 1, 2020

Язык: Английский

---

Integrating single-cell and spatial transcriptomics to elucidate intercellular tissue dynamics

Nature Reviews Genetics, Год журнала: 2021, Номер 22(10), С. 627 - 644

Опубликована: Июнь 18, 2021

Язык: Английский

---

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes

eLife, Год журнала: 2018, Номер 7

Опубликована: Июль 11, 2018

The architecture of normal and diseased tissues strongly influences the development progression disease as well responsiveness resistance to therapy. We describe a tissue-based cyclic immunofluorescence (t-CyCIF) method for highly multiplexed immuno-fluorescence imaging formalin-fixed, paraffin-embedded (FFPE) specimens mounted on glass slides, most widely used histopathological diagnosis cancer other diseases. t-CyCIF generates up 60-plex images using an iterative process (a cycle) in which conventional low-plex fluorescence are repeatedly collected from same sample then assembled into high-dimensional representation. requires no specialized instruments or reagents is compatible with super-resolution imaging; we demonstrate its application quantifying signal transduction cascades, tumor antigens immune markers diverse tumors. simplicity adaptability makes it effective pre-clinical clinical research natural complement single-cell genomics.

Язык: Английский

---

histoCAT: analysis of cell phenotypes and interactions in multiplex image cytometry data

Nature Methods, Год журнала: 2017, Номер 14(9), С. 873 - 876

Опубликована: Авг. 7, 2017

Язык: Английский

---

Ki67 is a Graded Rather than a Binary Marker of Proliferation versus Quiescence

Cell Reports, Год журнала: 2018, Номер 24(5), С. 1105 - 1112.e5

Опубликована: Июль 1, 2018

Ki67 staining is widely used as a proliferation indicator in the clinic, despite poor understanding of this protein's function or dynamics. Here, we track levels under endogenous control single cells over time and find that accumulation occurs only during S, G2, M phases. degraded continuously G1 G0 phases, regardless cause entry into G0/quiescence. Consequently, level individual highly heterogeneous depends on how long an cell has spent G0. Thus, graded rather than binary marker both for cell-cycle progression since quiescence.

Язык: Английский

---

Improving cancer immunotherapy through nanotechnology

Nature reviews. Cancer, Год журнала: 2019, Номер 19(10), С. 587 - 602

Опубликована: Сен. 6, 2019

Язык: Английский

---

Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity

Cell, Год журнала: 2020, Номер 183(7), С. 1848 - 1866.e26

Опубликована: Дек. 1, 2020

Язык: Английский

---

Multiplexed protein maps link subcellular organization to cellular states

Science, Год журнала: 2018, Номер 361(6401)

Опубликована: Авг. 3, 2018

Making multiplexed subcellular protein maps Being able to visualize localizations within cells and tissues by means of immuno-fluorescence microscopy has been key developments in cell biology beyond. Gut et al. present a high-throughput method that achieves the detection more than 40 different proteins biological samples across multiple spatial scales. This allows simultaneous quantification their expression levels thousands single cells; captures detailed distribution various compartments, organelles, cellular structures each these places all this information multicellular context. Such scale-crossing dataset empowers artificial intelligence–based computer vision algorithms achieve comprehensive profiling intracellular measure responses multicellular, cellular, pharmacological contexts, reveal new states. Science , issue p. eaar7042

Язык: Английский

---