High-dimensional single cell analysis identifies stem-like cytotoxic CD8+ T cells infiltrating human tumors DOI Creative Commons
Jolanda Brummelman, Emilia Maria Cristina Mazza, Giorgia Alvisi

и другие.

The Journal of Experimental Medicine, Год журнала: 2018, Номер 215(10), С. 2520 - 2535

Опубликована: Авг. 28, 2018

CD8+ T cells infiltrating tumors are largely dysfunctional, but whether a subset maintains superior functionality remains ill defined. By high-dimensional single cell analysis of millions from 53 individuals with lung cancer, we defined those subsets that enriched in compared cancer-free tissues and blood. Besides exhausted activated cells, identified CXCR5+ TIM-3– partial phenotype, while retaining gene networks responsible for stem-like plasticity cytotoxicity, as revealed by sequencing the whole transcriptome. Ex vivo, displayed enhanced self-renewal multipotency more differentiated were polyfunctional. Analysis inhibitory costimulatory receptors PD-1, TIGIT, CD27 possible targets immunotherapy. We thus demonstrate hierarchy differentiation context exhaustion human cancer similar to chronically infected mice, which is further shown disappear disease progression.

Язык: Английский

Targeting REGNASE-1 programs long-lived effector T cells for cancer therapy DOI
Jun Wei, Lingyun Long, Wenting Zheng

и другие.

Nature, Год журнала: 2019, Номер 576(7787), С. 471 - 476

Опубликована: Дек. 11, 2019

Язык: Английский

Процитировано

339
CXCR6 positions cytotoxic T cells to receive critical survival signals in the tumor microenvironment DOI Creative Commons
Mauro Di Pilato, Raphael Kfuri-Rubens, Jasper N. Pruessmann

и другие.

Cell, Год журнала: 2021, Номер 184(17), С. 4512 - 4530.e22

Опубликована: Авг. 1, 2021

Язык: Английский

Процитировано

326
Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) DOI Creative Commons
Andrea Cossarizza, Hyun‐Dong Chang, Andreas Radbruch

и другие.

European Journal of Immunology, Год журнала: 2021, Номер 51(12), С. 2708 - 3145

Опубликована: Дек. 1, 2021

Abstract The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes functional assays all major human murine immune cell subsets. Notably, contain helpful tables highlighting differences between cells. Another useful feature this is analysis clinical samples with examples applications in context autoimmune diseases, cancers as well acute chronic infectious diseases. Furthermore, there are detailing tips, tricks pitfalls avoid. All written peer‐reviewed by leading experts immunologists, making an essential state‐of‐the‐art handbook for basic researchers.

Язык: Английский

Процитировано

324
Neoantigen-driven B cell and CD4 T follicular helper cell collaboration promotes anti-tumor CD8 T cell responses DOI Creative Commons
Can Cui, Jiawei Wang, Eric Fagerberg

и другие.

Cell, Год журнала: 2021, Номер 184(25), С. 6101 - 6118.e13

Опубликована: Ноя. 30, 2021

Язык: Английский

Процитировано

321
High-dimensional single cell analysis identifies stem-like cytotoxic CD8+ T cells infiltrating human tumors DOI Creative Commons
Jolanda Brummelman, Emilia Maria Cristina Mazza, Giorgia Alvisi

и другие.

The Journal of Experimental Medicine, Год журнала: 2018, Номер 215(10), С. 2520 - 2535

Опубликована: Авг. 28, 2018

CD8+ T cells infiltrating tumors are largely dysfunctional, but whether a subset maintains superior functionality remains ill defined. By high-dimensional single cell analysis of millions from 53 individuals with lung cancer, we defined those subsets that enriched in compared cancer-free tissues and blood. Besides exhausted activated cells, identified CXCR5+ TIM-3– partial phenotype, while retaining gene networks responsible for stem-like plasticity cytotoxicity, as revealed by sequencing the whole transcriptome. Ex vivo, displayed enhanced self-renewal multipotency more differentiated were polyfunctional. Analysis inhibitory costimulatory receptors PD-1, TIGIT, CD27 possible targets immunotherapy. We thus demonstrate hierarchy differentiation context exhaustion human cancer similar to chronically infected mice, which is further shown disappear disease progression.

Язык: Английский

Процитировано

304