Biophysical Journal,
Год журнала:
2013,
Номер
105(3), С. 609 - 619
Опубликована: Авг. 1, 2013
In
eukaryotic
cells,
small
changes
in
cell
volume
can
serve
as
important
signals
for
proliferation,
death,
and
migration.
Volume
shape
regulation
also
directly
impacts
the
mechanics
of
cells
tissues.
Here,
we
develop
a
mathematical
model
cellular
pressure
regulation,
incorporating
essential
elements
such
water
permeation,
mechanosensitive
channels,
active
ion
pumps,
stresses
cortex.
The
fully
explain
recent
experimental
data,
it
predicts
several
models
cortical
mechanics.
Moreover,
show
that
when
are
subjected
to
an
externally
applied
load,
atomic
force
microscopy
indentation
experiment,
leads
complex
response.
Instead
passive
cortex,
observed
stiffness
depends
on
factors
working
together.
This
provides
explanation
rate-dependent
response
under
force.
Proceedings of the National Academy of Sciences,
Год журнала:
2017,
Номер
114(41)
Опубликована: Сен. 25, 2017
Significance
Cell
volume
is
thought
to
be
a
well-controlled
cellular
characteristic,
increasing
as
cell
grows,
while
macromolecular
density
maintained.
We
report
that
can
also
change
in
response
external
physical
cues,
leading
water
influx/efflux,
which
causes
significant
changes
subcellular
density.
This
observed
when
cells
spread
out
on
substrate:
Cells
reduce
their
and
increase
molecular
crowding
due
an
accompanying
efflux.
Exploring
this
phenomenon
further,
we
removed
from
mesenchymal
stem
through
osmotic
pressure
found
was
sufficient
alter
differentiation
pathway.
Based
these
results,
suggest
chart
different
behavioral
pathways
by
sensing/altering
cytoplasmic
influx/efflux.
Journal of The Royal Society Interface,
Год журнала:
2015,
Номер
12(104), С. 20140970 - 20140970
Опубликована: Янв. 15, 2015
Transmission
of
mechanical
force
is
crucial
for
normal
cell
development
and
functioning.
However,
the
process
mechanotransduction
cannot
be
studied
in
isolation
from
mechanics.
Thus,
order
to
understand
how
cells
'feel',
we
must
first
they
deform
recover
physical
perturbations.
Owing
its
versatility,
atomic
microscopy
(AFM)
has
become
a
popular
tool
study
intrinsic
cellular
properties.
Used
directly
manipulate
examine
whole
subcellular
reactions,
AFM
allows
top-down
reconstitutive
approaches
characterization.
These
studies
show
that
responses
their
components
are
complex,
largely
depend
on
magnitude
time
scale
loading.
In
this
review,
generally
describe
mechanotransductive
through
discussion
well-known
mechanosensors.
We
then
focus
recent
examples
where
used
specifically
probe
elastic
inelastic
single
undergoing
deformation.
present
brief
overview
classical
current
models
often
characterize
observed
phenomena
response
force.
Both
simple
mechanistic
complex
nonlinear
have
been
behaviours,
however
unifying
description
mechanics
not
yet
resolved.
Chemical Society Reviews,
Год журнала:
2020,
Номер
49(16), С. 5850 - 5884
Опубликована: Янв. 1, 2020
This
review
provides
an
introduction
to
the
state-of-the-art
force
microscope
methods
map
at
high-spatial
resolution
elastic
and
viscoelastic
properties
of
proteins,
polymers
cells.
Biology of the Cell,
Год журнала:
2017,
Номер
109(5), С. 167 - 189
Опубликована: Фев. 28, 2017
Solid
tumours
are
often
first
diagnosed
by
palpation,
suggesting
that
the
tumour
is
more
rigid
than
its
surrounding
environment.
Paradoxically,
individual
cancer
cells
appear
to
be
softer
their
healthy
counterparts.
In
this
review,
we
list
physiological
reasons
indicating
may
deformable
normal
cells.
Next,
describe
biophysical
tools
have
been
developed
in
recent
years
characterise
and
model
cell
mechanics.
By
reviewing
experimental
studies
compared
mechanics
of
cells,
argue
can
indeed
considered
as
We
then
focus
on
intracellular
elements
could
responsible
for
softening
Finally,
ask
whether
mechanical
differences
between
used
diagnostic
or
prognostic
markers
progression.
