Neuron,
Год журнала:
2019,
Номер
102(5), С. 1009 - 1024.e8
Опубликована: Апрель 29, 2019
Maintaining
average
activity
within
a
set-point
range
constitutes
fundamental
property
of
central
neural
circuits.
However,
whether
and
how
set
points
are
regulated
remains
unknown.
Integrating
genome-scale
metabolic
modeling
experimental
study
neuronal
homeostasis,
we
identified
mitochondrial
dihydroorotate
dehydrogenase
(DHODH)
as
regulator
in
hippocampal
networks.
The
DHODH
inhibitor
teriflunomide
stably
suppressed
mean
firing
rates
via
synaptic
intrinsic
excitability
mechanisms
by
modulating
Ca
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2016,
Номер
unknown
Опубликована: Июнь 30, 2016
Abstract
Two-photon
microscopy
of
calcium-dependent
sensors
has
enabled
unprecedented
recordings
from
vast
populations
neurons.
While
the
and
microscopes
have
matured
over
several
generations
development,
computational
methods
to
process
resulting
movies
remain
inefficient
can
give
results
that
are
hard
interpret.
Here
we
introduce
Suite2p:
a
fast,
accurate
complete
pipeline
registers
raw
movies,
detects
active
cells,
extracts
their
calcium
traces
infers
spike
times.
Suite2p
runs
on
standard
workstations,
operates
faster
than
real
time,
recovers
~2
times
more
cells
previous
state-of-the-art
method.
Its
low
load
allows
routine
detection
~10,000
simultaneously
with
two-photon
resonant-scanning
microscopes.
Recordings
at
this
scale
promise
reveal
fine
structure
activity
in
large
neurons
or
subcellular
structures
such
as
synaptic
boutons.
Neuron,
Год журнала:
2018,
Номер
98(2), С. 256 - 281
Опубликована: Апрель 1, 2018
Tremendous
progress
has
been
made
since
Neuron
published
our
Primer
on
genetic
dissection
of
neural
circuits
10
years
ago.
Since
then,
cell-type-specific
anatomical,
neurophysiological,
and
perturbation
studies
have
carried
out
in
a
multitude
invertebrate
vertebrate
organisms,
linking
neurons
to
behavioral
functions.
New
methods
allow
systematic
classification
cell
types
provide
access
diverse
neuronal
for
connectivity
coding
during
behavior.
Here
we
evaluate
key
advances
over
the
past
decade
discuss
future
directions.
Cell Reports,
Год журнала:
2017,
Номер
21(4), С. 1102 - 1115
Опубликована: Окт. 1, 2017
Ca2+
imaging
techniques
permit
time-lapse
recordings
of
neuronal
activity
from
large
populations
over
weeks.
However,
without
identifying
the
same
neurons
across
sessions
(cell
registration),
longitudinal
analysis
neural
code
is
restricted
to
population-level
statistics.
Accurate
cell
registration
becomes
challenging
with
increased
numbers
cells,
sessions,
and
inter-session
intervals.
Current
practices,
whether
manual
or
automatic,
do
not
quantitatively
evaluate
accuracy,
possibly
leading
data
misinterpretation.
We
developed
a
probabilistic
method
that
automatically
registers
cells
multiple
estimates
confidence
for
each
registered
cell.
Using
large-scale
recorded
weeks
hippocampus
cortex
freely
behaving
mice,
we
show
our
performs
more
accurate
than
previously
used
routines,
yielding
estimated
error
rates
<5%,
scalable
many
sessions.
Thus,
allows
reliable
long
time
periods.
Current Opinion in Neurobiology,
Год журнала:
2018,
Номер
50, С. 92 - 100
Опубликована: Фев. 13, 2018
Electrophysiological
methods
are
the
gold
standard
in
neuroscience
because
they
reveal
activity
of
individual
neurons
at
high
temporal
resolution
and
arbitrary
brain
locations.
Microelectrode
arrays
based
on
complementary
metal-oxide
semiconductor
(CMOS)
technology,
such
as
Neuropixels
probes,
look
set
to
transform
these
methods.
probes
provide
∼1000
recording
sites
an
extremely
narrow
shank,
with
on-board
amplification,
digitization,
multiplexing.
They
deliver
low-noise
recordings
from
hundreds
neurons,
providing
a
step
change
type
data
available
neuroscientists.
Here
we
discuss
opportunities
afforded
by
for
large-scale
electrophysiology,
challenges
associated
processing
anatomical
localization,
avenues
further
improvements
technology.
Cell Reports,
Год журнала:
2016,
Номер
17(12), С. 3385 - 3394
Опубликована: Дек. 1, 2016
A
major
technological
goal
in
neuroscience
is
to
enable
the
interrogation
of
individual
cells
across
live
brain.
By
creating
a
curved
glass
replacement
dorsal
cranium
and
surgical
methods
for
its
installation,
we
developed
chronic
mouse
preparation
providing
optical
access
an
estimated
800,000–1,100,000
neurons
surface
neocortex.
Post-surgical
histological
studies
revealed
comparable
glial
activation
as
control
mice.
In
behaving
mice
expressing
Ca2+
indicator
cortical
pyramidal
neurons,
performed
imaging
neocortex
using
epi-fluorescence
macroscope
that
25,000–50,000
were
accessible
per
multiple
focal
planes.
Two-photon
microscopy
dendritic
morphologies
throughout
neocortex,
allowed
time-lapse
cells,
yielded
estimates
>1
million
by
serial
tiling.
This
approach
supports
variety
techniques
enables
>30
neocortical
areas
