Mitochondrial Regulation of the Hippocampal Firing Rate Set Point and Seizure Susceptibility DOI Creative Commons

Boaz Styr,

N Gonen,

Daniel Zarhin

и другие.

Neuron, Год журнала: 2019, Номер 102(5), С. 1009 - 1024.e8

Опубликована: Апрель 29, 2019

Maintaining average activity within a set-point range constitutes fundamental property of central neural circuits. However, whether and how set points are regulated remains unknown. Integrating genome-scale metabolic modeling experimental study neuronal homeostasis, we identified mitochondrial dihydroorotate dehydrogenase (DHODH) as regulator in hippocampal networks. The DHODH inhibitor teriflunomide stably suppressed mean firing rates via synaptic intrinsic excitability mechanisms by modulating Ca

Язык: Английский

Suite2p: beyond 10,000 neurons with standard two-photon microscopy DOI Creative Commons
Marius Pachitariu, Carsen Stringer, Mario Dipoppa

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2016, Номер unknown

Опубликована: Июнь 30, 2016

Abstract Two-photon microscopy of calcium-dependent sensors has enabled unprecedented recordings from vast populations neurons. While the and microscopes have matured over several generations development, computational methods to process resulting movies remain inefficient can give results that are hard interpret. Here we introduce Suite2p: a fast, accurate complete pipeline registers raw movies, detects active cells, extracts their calcium traces infers spike times. Suite2p runs on standard workstations, operates faster than real time, recovers ~2 times more cells previous state-of-the-art method. Its low load allows routine detection ~10,000 simultaneously with two-photon resonant-scanning microscopes. Recordings at this scale promise reveal fine structure activity in large neurons or subcellular structures such as synaptic boutons.

Язык: Английский

Процитировано

1107

Novel electrode technologies for neural recordings DOI
Guosong Hong, Charles M. Lieber

Nature reviews. Neuroscience, Год журнала: 2019, Номер 20(6), С. 330 - 345

Опубликована: Март 4, 2019

Язык: Английский

Процитировано

597

Genetic Dissection of Neural Circuits: A Decade of Progress DOI Creative Commons
Liqun Luo, Edward M. Callaway, Karel Svoboda

и другие.

Neuron, Год журнала: 2018, Номер 98(2), С. 256 - 281

Опубликована: Апрель 1, 2018

Tremendous progress has been made since Neuron published our Primer on genetic dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical, neurophysiological, and perturbation studies have carried out in a multitude invertebrate vertebrate organisms, linking neurons to behavioral functions. New methods allow systematic classification cell types provide access diverse neuronal for connectivity coding during behavior. Here we evaluate key advances over the past decade discuss future directions.

Язык: Английский

Процитировано

453

A Fully Automated Approach to Spike Sorting DOI Creative Commons
Jason E. Chung, Jeremy F. Magland,

Alex H. Barnett

и другие.

Neuron, Год журнала: 2017, Номер 95(6), С. 1381 - 1394.e6

Опубликована: Сен. 1, 2017

Язык: Английский

Процитировано

451

Tracking the Same Neurons across Multiple Days in Ca2+ Imaging Data DOI Creative Commons
Liron Sheintuch,

Alon Rubin,

Noa Brande-Eilat

и другие.

Cell Reports, Год журнала: 2017, Номер 21(4), С. 1102 - 1115

Опубликована: Окт. 1, 2017

Ca2+ imaging techniques permit time-lapse recordings of neuronal activity from large populations over weeks. However, without identifying the same neurons across sessions (cell registration), longitudinal analysis neural code is restricted to population-level statistics. Accurate cell registration becomes challenging with increased numbers cells, sessions, and inter-session intervals. Current practices, whether manual or automatic, do not quantitatively evaluate accuracy, possibly leading data misinterpretation. We developed a probabilistic method that automatically registers cells multiple estimates confidence for each registered cell. Using large-scale recorded weeks hippocampus cortex freely behaving mice, we show our performs more accurate than previously used routines, yielding estimated error rates <5%, scalable many sessions. Thus, allows reliable long time periods.

Язык: Английский

Процитировано

351

Challenges and opportunities for large-scale electrophysiology with Neuropixels probes DOI Creative Commons
Nicholas A. Steinmetz, Christof Koch, Kenneth D. Harris

и другие.

Current Opinion in Neurobiology, Год журнала: 2018, Номер 50, С. 92 - 100

Опубликована: Фев. 13, 2018

Electrophysiological methods are the gold standard in neuroscience because they reveal activity of individual neurons at high temporal resolution and arbitrary brain locations. Microelectrode arrays based on complementary metal-oxide semiconductor (CMOS) technology, such as Neuropixels probes, look set to transform these methods. probes provide ∼1000 recording sites an extremely narrow shank, with on-board amplification, digitization, multiplexing. They deliver low-noise recordings from hundreds neurons, providing a step change type data available neuroscientists. Here we discuss opportunities afforded by for large-scale electrophysiology, challenges associated processing anatomical localization, avenues further improvements technology.

Язык: Английский

Процитировано

316

Technologies for imaging neural activity in large volumes DOI
Na Ji, Jeremy Freeman, Spencer L. Smith

и другие.

Nature Neuroscience, Год журнала: 2016, Номер 19(9), С. 1154 - 1164

Опубликована: Авг. 26, 2016

Язык: Английский

Процитировано

275

Large-scale neural recordings call for new insights to link brain and behavior DOI
Anne E. Urai, Brent Doiron, Andrew M. Leifer

и другие.

Nature Neuroscience, Год журнала: 2022, Номер 25(1), С. 11 - 19

Опубликована: Янв. 1, 2022

Язык: Английский

Процитировано

259

Long-Term Optical Access to an Estimated One Million Neurons in the Live Mouse Cortex DOI Creative Commons
Tony Hyun Kim, Yanping Zhang, Jérôme Lecoq

и другие.

Cell Reports, Год журнала: 2016, Номер 17(12), С. 3385 - 3394

Опубликована: Дек. 1, 2016

A major technological goal in neuroscience is to enable the interrogation of individual cells across live brain. By creating a curved glass replacement dorsal cranium and surgical methods for its installation, we developed chronic mouse preparation providing optical access an estimated 800,000–1,100,000 neurons surface neocortex. Post-surgical histological studies revealed comparable glial activation as control mice. In behaving mice expressing Ca2+ indicator cortical pyramidal neurons, performed imaging neocortex using epi-fluorescence macroscope that 25,000–50,000 were accessible per multiple focal planes. Two-photon microscopy dendritic morphologies throughout neocortex, allowed time-lapse cells, yielded estimates >1 million by serial tiling. This approach supports variety techniques enables >30 neocortical areas

Язык: Английский

Процитировано

258

Accurate Estimation of Neural Population Dynamics without Spike Sorting DOI Creative Commons
Eric M. Trautmann, Sergey D. Stavisky, Subhaneil Lahiri

и другие.

Neuron, Год журнала: 2019, Номер 103(2), С. 292 - 308.e4

Опубликована: Июнь 3, 2019

Язык: Английский

Процитировано

242