Neuronal cell-type classification: challenges, opportunities and the path forward

Nature reviews. Neuroscience, Год журнала: 2017, Номер 18(9), С. 530 - 546

Опубликована: Авг. 3, 2017

Язык: Английский

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Genetic Dissection of Neural Circuits: A Decade of Progress

Neuron, Год журнала: 2018, Номер 98(2), С. 256 - 281

Опубликована: Апрель 1, 2018

Tremendous progress has been made since Neuron published our Primer on genetic dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical, neurophysiological, and perturbation studies have carried out in a multitude invertebrate vertebrate organisms, linking neurons to behavioral functions. New methods allow systematic classification cell types provide access diverse neuronal for connectivity coding during behavior. Here we evaluate key advances over the past decade discuss future directions.

Язык: Английский

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In vivo imaging of neural activity

Nature Methods, Год журнала: 2017, Номер 14(4), С. 349 - 359

Опубликована: Март 31, 2017

Язык: Английский

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Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

Nature Methods, Год журнала: 2020, Номер 17(3), С. 287 - 290

Опубликована: Март 2, 2020

Язык: Английский

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High-speed volumetric imaging of neuronal activity in freely moving rodents

Nature Methods, Год журнала: 2018, Номер 15(6), С. 429 - 432

Опубликована: Май 4, 2018

Язык: Английский

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Metasurfaces for biomedical applications: imaging and sensing from a nanophotonics perspective

Nanophotonics, Год журнала: 2020, Номер 10(1), С. 259 - 293

Опубликована: Сен. 7, 2020

Abstract Metasurface is a recently developed nanophotonics concept to manipulate the properties of light by replacing conventional bulky optical components with ultrathin (more than 10 4 times thinner) flat components. Since first demonstration metasurfaces in 2011, they have attracted tremendous interest consumer optics and electronics industries. Recently, metasurface-empowered novel bioimaging biosensing tools emerged been reported. Given recent advances biomedical engineering, this review article covers state art for technology provides comprehensive interdisciplinary perspective on field. The topics that we covered include chiral imaging, endoscopic coherence tomography, fluorescent super-resolution magnetic resonance quantitative phase sensing antibodies, proteins, DNAs, cells, cancer biomarkers. Future directions are discussed twofold: application-specific bioinspired metasurface devices. Perspectives challenges opportunities metasurfaces, biophotonics, translational devices also provided. objective inform stimulate research: firstly, introducing community; secondly assisting community understand needs realize medical fields. In addition, two knowledge boxes describing design process lens performance matrix biosensor, which serve as “crash-course” introduction those new both

Язык: Английский

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Volumetric Ca2+ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy

Cell, Год журнала: 2019, Номер 177(4), С. 1050 - 1066.e14

Опубликована: Апрель 11, 2019

Язык: Английский

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Functional clustering of dendritic activity during decision-making

eLife, Год журнала: 2019, Номер 8

Опубликована: Окт. 28, 2019

The active properties of dendrites can support local nonlinear operations, but previous imaging and electrophysiological measurements have produced conflicting views regarding the prevalence selectivity nonlinearities in vivo. We imaged calcium signals pyramidal cell motor cortex mice performing a tactile decision task. A custom microscope allowed us to image soma up 300 μm contiguous dendrite at 15 Hz, while resolving individual spines. New analysis methods were used estimate frequency spatial scales activity dendritic branches majority transients coincident with global events. However, task-associated spines compartmentalized by branching clustered within over approximately 10 μm. Diverse behavior-related intermingled distributed throughout arbor, potentially supporting large learning capacity neurons.

Язык: Английский

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Kilohertz frame-rate two-photon tomography

Nature Methods, Год журнала: 2019, Номер 16(8), С. 778 - 786

Опубликована: Июль 29, 2019

Язык: Английский

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Fast near-whole–brain imaging in adult Drosophila during responses to stimuli and behavior

PLoS Biology, Год журнала: 2019, Номер 17(2), С. e2006732 - e2006732

Опубликована: Фев. 15, 2019

Whole-brain recordings give us a global perspective of the brain in action. In this study, we describe method using light field microscopy to record near-whole calcium and voltage activity at high speed behaving adult flies. We first obtained maps for various stimuli behaviors. Notably, found that increased on scale when fly walked but not it groomed. This increase with walking was particularly strong dopamine neurons. Second, extracted spatially distinct sources as well their time series principal component analysis independent analysis. The characteristic shapes matched anatomy subneuropil regions and, some cases, specific neuron type. Brain structures responded odor were consistent previous reports, confirming new technique's validity. also observed previously uncharacterized behavior-related patterns spontaneous activity.

Язык: Английский

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