Nature Reviews Genetics, Год журнала: 2018, Номер 19(6), С. 385 - 397
Опубликована: Март 19, 2018
Язык: Английский
Nature Reviews Genetics, Год журнала: 2018, Номер 19(6), С. 385 - 397
Опубликована: Март 19, 2018
Язык: Английский
Nature reviews. Cancer, Год журнала: 2015, Номер 15(10), С. 593 - 607
Опубликована: Сен. 18, 2015
Язык: Английский
Процитировано
462Nature Reviews Molecular Cell Biology, Год журнала: 2017, Номер 18(4), С. 263 - 273
Опубликована: Март 1, 2017
Язык: Английский
Процитировано
452Molecular Cell, Год журнала: 2017, Номер 67(1), С. 5 - 18.e19
Опубликована: Июнь 29, 2017
Язык: Английский
Процитировано
416Cell, Год журнала: 2017, Номер 169(2), С. 326 - 337.e12
Опубликована: Апрель 1, 2017
Transcription and translation are two main pillars of gene expression. Due to the different timings, spots action, mechanisms regulation, these processes mainly regarded as distinct generally uncoupled, despite serving a common purpose. Here, we sought for possible connection between transcription translation. Employing an unbiased screen multiple human promoters, identified positive effect TATA box on general coupling mRNA expression translational efficiency. Using CRISPR-Cas9-mediated approach, genome-wide analyses, in vitro experiments, show that rate regulates efficiency Furthermore, demonstrate m6A modification mRNAs is co-transcriptional depends upon dynamics transcribing RNAPII. Suboptimal rates lead elevated content, which may result reduced This study uncovers widespread link governed by epigenetic mRNAs.
Язык: Английский
Процитировано
414Nature Medicine, Год журнала: 2017, Номер 23(4), С. 493 - 500
Опубликована: Фев. 27, 2017
Язык: Английский
Процитировано
405eLife, Год журнала: 2016, Номер 5
Опубликована: Март 17, 2016
The prokaryotic CRISPR (clustered regularly interspaced palindromic repeats)-associated protein, Cas9, has been widely adopted as a tool for editing, imaging, and regulating eukaryotic genomes. However, our understanding of how to select single-guide RNAs (sgRNAs) that mediate efficient Cas9 activity is incomplete, we lack insight into chromatin impacts targeting. To address this gap, analyzed large-scale genetic screens performed in human cell lines using either nuclease-active or nuclease-dead (dCas9). We observed highly active sgRNAs dCas9 were found almost exclusively regions low nucleosome occupancy. In vitro experiments demonstrated nucleosomes fact directly impede binding cleavage, while remodeling can restore access. Our results reveal critical role dictating the targeting specificity transplanted bacterial enzyme, provide rules selecting target sites distinct from complementary those based on sequence properties.
Язык: Английский
Процитировано
387Molecular Cell, Год журнала: 2017, Номер 68(4), С. 745 - 757.e5
Опубликована: Ноя. 1, 2017
Язык: Английский
Процитировано
334Nature, Год журнала: 2016, Номер 539(7629), С. 433 - 436
Опубликована: Окт. 25, 2016
Язык: Английский
Процитировано
330Nature Reviews Molecular Cell Biology, Год журнала: 2017, Номер 18(10), С. 637 - 650
Опубликована: Авг. 9, 2017
Язык: Английский
Процитировано
330Science Advances, Год журнала: 2015, Номер 1(11)
Опубликована: Дек. 4, 2015
The Polycomb group (PcG) of proteins defines a subset factors that physically associate and function to maintain the positional identity cells from embryo adult stages. PcG has long been considered paradigmatic model for epigenetic maintenance gene transcription programs. Despite intensive research efforts unveil molecular mechanisms action proteins, several fundamental questions remain unresolved: How many different complexes exist in mammalian cells? are targeted specific loci? does regulate transcription? In this review, we discuss diversity cells, examine newly identified modes recruitment chromatin, highlight latest insights into underlying PcGs regulation three-dimensional chromatin conformation.
Язык: Английский
Процитировано
328