MYC: connecting selective transcriptional control to global RNA production

Nature reviews. Cancer, Год журнала: 2015, Номер 15(10), С. 593 - 607

Опубликована: Сен. 18, 2015

Язык: Английский

---

The code and beyond: transcription regulation by the RNA polymerase II carboxy-terminal domain

Nature Reviews Molecular Cell Biology, Год журнала: 2017, Номер 18(4), С. 263 - 273

Опубликована: Март 1, 2017

Язык: Английский

---

BET Bromodomain Proteins Function as Master Transcription Elongation Factors Independent of CDK9 Recruitment

Molecular Cell, Год журнала: 2017, Номер 67(1), С. 5 - 18.e19

Опубликована: Июнь 29, 2017

Язык: Английский

---

Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation

Cell, Год журнала: 2017, Номер 169(2), С. 326 - 337.e12

Опубликована: Апрель 1, 2017

Transcription and translation are two main pillars of gene expression. Due to the different timings, spots action, mechanisms regulation, these processes mainly regarded as distinct generally uncoupled, despite serving a common purpose. Here, we sought for possible connection between transcription translation. Employing an unbiased screen multiple human promoters, identified positive effect TATA box on general coupling mRNA expression translational efficiency. Using CRISPR-Cas9-mediated approach, genome-wide analyses, in vitro experiments, show that rate regulates efficiency Furthermore, demonstrate m6A modification mRNAs is co-transcriptional depends upon dynamics transcribing RNAPII. Suboptimal rates lead elevated content, which may result reduced This study uncovers widespread link governed by epigenetic mRNAs.

Язык: Английский

---

Therapeutic targeting of polycomb and BET bromodomain proteins in diffuse intrinsic pontine gliomas

Nature Medicine, Год журнала: 2017, Номер 23(4), С. 493 - 500

Опубликована: Фев. 27, 2017

Язык: Английский

---

Nucleosomes impede Cas9 access to DNA in vivo and in vitro

eLife, Год журнала: 2016, Номер 5

Опубликована: Март 17, 2016

The prokaryotic CRISPR (clustered regularly interspaced palindromic repeats)-associated protein, Cas9, has been widely adopted as a tool for editing, imaging, and regulating eukaryotic genomes. However, our understanding of how to select single-guide RNAs (sgRNAs) that mediate efficient Cas9 activity is incomplete, we lack insight into chromatin impacts targeting. To address this gap, analyzed large-scale genetic screens performed in human cell lines using either nuclease-active or nuclease-dead (dCas9). We observed highly active sgRNAs dCas9 were found almost exclusively regions low nucleosome occupancy. In vitro experiments demonstrated nucleosomes fact directly impede binding cleavage, while remodeling can restore access. Our results reveal critical role dictating the targeting specificity transplanted bacterial enzyme, provide rules selecting target sites distinct from complementary those based on sequence properties.

Язык: Английский

---

R-ChIP Using Inactive RNase H Reveals Dynamic Coupling of R-loops with Transcriptional Pausing at Gene Promoters

Molecular Cell, Год журнала: 2017, Номер 68(4), С. 745 - 757.e5

Опубликована: Ноя. 1, 2017

Язык: Английский

---

Transcription of the non-coding RNA upperhand controls Hand2 expression and heart development

Nature, Год журнала: 2016, Номер 539(7629), С. 433 - 436

Опубликована: Окт. 25, 2016

Язык: Английский

---

Splicing and transcription touch base: co-transcriptional spliceosome assembly and function

Nature Reviews Molecular Cell Biology, Год журнала: 2017, Номер 18(10), С. 637 - 650

Опубликована: Авг. 9, 2017

Язык: Английский

---

Regulation of gene transcription by Polycomb proteins

Science Advances, Год журнала: 2015, Номер 1(11)

Опубликована: Дек. 4, 2015

The Polycomb group (PcG) of proteins defines a subset factors that physically associate and function to maintain the positional identity cells from embryo adult stages. PcG has long been considered paradigmatic model for epigenetic maintenance gene transcription programs. Despite intensive research efforts unveil molecular mechanisms action proteins, several fundamental questions remain unresolved: How many different complexes exist in mammalian cells? are targeted specific loci? does regulate transcription? In this review, we discuss diversity cells, examine newly identified modes recruitment chromatin, highlight latest insights into underlying PcGs regulation three-dimensional chromatin conformation.

Язык: Английский

---