Multidrug-resistant
bacteria
pose
a
serious
health
threat,
especially
in
hospitals.
Horizontal
gene
transfer
(HGT)
of
mobile
genetic
elements
(MGEs)
facilitates
the
spread
antibiotic
resistance,
virulence,
and
environmental
persistence
genes
between
nosocomial
pathogens.
We
screened
genomes
2173
bacterial
isolates
from
healthcare-associated
infections
single
hospital
over
18
months,
identified
identical
nucleotide
regions
belonging
to
distinct
genera.
To
further
resolve
these
shared
sequences,
we
performed
long-read
sequencing
on
subset
generated
highly
contiguous
genomes.
then
tracked
appearance
ten
different
plasmids
all
genomes,
found
evidence
plasmid
independent
transmission.
Finally,
two
instances
likely
within
individual
patients,
including
one
that
transferred
second
patient.
This
work
expands
our
understanding
HGT
healthcare
settings,
can
inform
efforts
limit
drug-resistant
pathogens
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research,
Год журнала:
2020,
Номер
1868(1), С. 118896 - 118896
Опубликована: Окт. 21, 2020
Vitamin
B12,
cobalamin,
is
a
cobalt-containing
ring-contracted
modified
tetrapyrrole
that
represents
one
of
the
most
complex
small
molecules
made
by
nature.
In
prokaryotes
it
utilised
as
cofactor,
coenzyme,
light
sensor
and
gene
regulator
yet
has
restricted
role
in
assisting
only
two
enzymes
within
specific
eukaryotes
including
mammals.
This
deployment
disparity
reflected
another
unique
attribute
vitamin
B12
its
biosynthesis
limited
to
certain
prokaryotes,
with
synthesisers
pivotal
establishing
mutualistic
microbial
communities.
The
core
component
cobalamin
corrin
macrocycle
acts
main
ligand
for
cobalt.
Within
this
review
we
investigate
why
cobalt
paired
specifically
ring,
how
inserted
during
biosynthetic
process,
available
cell
explore
cellular
control
levels.
partitioning
exemplifies
cells
assist
metalation.
Computational and Structural Biotechnology Journal,
Год журнала:
2020,
Номер
19, С. 94 - 109
Опубликована: Дек. 16, 2020
Heavy
metal(loid)s
exert
selective
pressure
on
microbial
communities
and
evolution
of
metal
resistance
determinants.
Despite
increasing
knowledge
concerning
the
impact
pollution
community
ecological
function,
it
is
still
a
challenge
to
identify
consistent
pattern
composition
along
gradients
elevated
in
natural
environments.
Further,
our
current
resistome
at
level
has
been
lagging
behind
compared
state-of-the-art
genetic
profiling
bacterial
mechanisms
pure
culture
system.
This
review
provides
an
overview
core
resistant
microbiome,
development
strategies,
potential
factors
driving
diversity
distribution
determinants
The
impacts
biotic
regulating
are
highlighted.
We
finally
discuss
advances
multiple
technologies,
research
challenges,
future
directions
better
understand
interface
environmental
microbiome
with
resistome.
aims
highlight
wide
heavy
their
corresponding
determinants,
helping
strategy
level.
Multidrug-resistant
bacteria
pose
a
serious
health
threat,
especially
in
hospitals.
Horizontal
gene
transfer
(HGT)
of
mobile
genetic
elements
(MGEs)
facilitates
the
spread
antibiotic
resistance,
virulence,
and
environmental
persistence
genes
between
nosocomial
pathogens.
We
screened
genomes
2173
bacterial
isolates
from
healthcare-associated
infections
single
hospital
over
18
months,
identified
identical
nucleotide
regions
belonging
to
distinct
genera.
To
further
resolve
these
shared
sequences,
we
performed
long-read
sequencing
on
subset
generated
highly
contiguous
genomes.
then
tracked
appearance
ten
different
plasmids
all
genomes,
found
evidence
plasmid
independent
transmission.
Finally,
two
instances
likely
within
individual
patients,
including
one
that
transferred
second
patient.
This
work
expands
our
understanding
HGT
healthcare
settings,
can
inform
efforts
limit
drug-resistant
pathogens
