Tricarboxylic Acid (TCA) Cycle Intermediates: Regulators of Immune Responses

Life, Год журнала: 2021, Номер 11(1), С. 69 - 69

Опубликована: Янв. 19, 2021

The tricarboxylic acid cycle (TCA) is a series of chemical reactions used in aerobic organisms to generate energy via the oxidation acetylcoenzyme A (CoA) derived from carbohydrates, fatty acids and proteins. In eukaryotic system, TCA occurs completely mitochondria, while intermediates are retained inside mitochondria due their polarity hydrophilicity. Under cell stress conditions, can become disrupted release contents, which act as danger signals cytosol. Of note, may also leak dysfunctioning regulate cellular processes. Increasing evidence shows that metabolites substantially involved regulation immune responses. this review, we aimed provide comprehensive systematic overview molecular mechanisms each intermediate play key roles regulating immunity discuss its implication for activation suppression.

Язык: Английский

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Identifying drug targets in tissues and whole blood with thermal-shift profiling

Nature Biotechnology, Год журнала: 2020, Номер 38(3), С. 303 - 308

Опубликована: Янв. 20, 2020

Язык: Английский

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Berberine Protects Glomerular Podocytes via Inhibiting Drp1-Mediated Mitochondrial Fission and Dysfunction

Theranostics, Год журнала: 2019, Номер 9(6), С. 1698 - 1713

Опубликована: Янв. 1, 2019

Elevated levels of plasma free fatty acid (FFA) and disturbed mitochondrial dynamics play crucial roles in the pathogenesis diabetic kidney disease (DKD).However, mechanisms by which FFA leads to damage glomerular podocytes DKD effects Berberine (BBR) on are not fully understood.Methods: Using db/db mice model cultured mouse podocytes, we investigated molecular mechanism FFA-induced disturbance testified BBR regulating dysfunction, podocyte apoptosis glomerulopathy progression DKD.Results: Intragastric administration for 8 weeks significantly reversed glucose lipid metabolism disorders, damage, basement membrane thickening, mesangial expansion glomerulosclerosis.BBR strongly inhibited apoptosis, increased reactive oxygen species (ROS) generation, fragmentation dysfunction both vivo vitro.Mechanistically, could stabilize morphology via abolishing palmitic (PA)-induced activation dynamin-related protein 1 (Drp1).Conclusions: Our study demonstrated first time that may have a previously unrecognized role protecting glomerulus positively Drp1-mediated dynamics.It might serve as novel therapeutic drug treatment DKD.

Язык: Английский

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Pathophysiology of AKI to CKD progression

Seminars in Nephrology, Год журнала: 2020, Номер 40(2), С. 206 - 215

Опубликована: Март 1, 2020

Язык: Английский

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Mitochondria transfer from mesenchymal stem cells structurally and functionally repairs renal proximal tubular epithelial cells in diabetic nephropathy in vivo

Scientific Reports, Год журнала: 2019, Номер 9(1)

Опубликована: Март 26, 2019

Abstract The underlying therapeutic mechanism of renal tubular epithelium repair diabetic nephropathy (DN) by bone marrow-derived mesenchymal stem cells (BM-MSCs) has not been fully elucidated. Recently, mitochondria (Mt) transfer was reported as a novel action BM-MSCs to rescue injured cells. We investigated Mt from systemically administered proximal epithelial (PTECs) in streptozotocin (STZ)-induced animals. also transferred their impaired PTECs when co-cultured vitro , which suppressed apoptosis PTECs. Additionally, BM-MSC-derived isolated enhanced the expression mitochondrial superoxide dismutase 2 and Bcl-2 inhibited reactive oxygen species (ROS) production . Isolated nuclear translocation PGC-1α restored megalin SGLT2 under high glucose condition (HG) Moreover, directly injected capsule STZ rats improved cellular morphology STZ-PTECs, structure basement membrane brush border vivo This study is first show damaged investigate mechanisms potential effects DN.

Язык: Английский

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