The RNA Revolution in the Central Molecular Biology Dogma Evolution DOI Open Access
William A. Haseltine, Roberto Patarca

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12695 - 12695

Опубликована: Ноя. 26, 2024

Human genome projects in the 1990s identified about 20,000 protein-coding sequences. We are now RNA revolution, propelled by realization that genes determine phenotype beyond foundational central molecular biology dogma, stating inherited linear pieces of DNA transcribed to RNAs and translated into proteins. Crucially, over 95% genome, initially considered junk between genes, encodes essential, functionally diverse non-protein-coding RNAs, raising gene count at least one order magnitude. Most phenotype-determining changes regulatory areas control can directly or indirectly phenotypes regulating protein function, transferring information within organisms, generating DNA. also exhibit high structural, functional, biomolecular interaction plasticity modified via editing, methylation, glycosylation, other mechanisms, which bestow them with intra- extracellular functions without altering underlying is, therefore, currently primary determinant cellular populational functional diversity, disease-linked structural variations, cell function regulation. As demonstrated RNA-based coronavirus vaccines' success, technology is transforming medicine, agriculture, industry, as did advent recombinant 1980s.

Язык: Английский

Small Interfering RNAs as Critical Regulators of Plant Life Process: New Perspectives on Regulating the Transcriptomic Machinery DOI Open Access
Marta Puchta, Paulina Bolc, Aleksandra Pietrusińska

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1624 - 1624

Опубликована: Фев. 14, 2025

Small interfering RNAs (siRNAs) are a distinct class of regulatory in plants and animals. Gene silencing by small is one the fundamental mechanisms for regulating gene expression. siRNAs critical regulators during developmental processes. have similar structures functions to but derived from double-stranded RNA may be involved directing DNA methylation target sequences. less well-studied than miRNA group, researchers continue identify new classes that appear at specific stages particular tissues, revealing more complex mode siRNA action previously thought. This review characterizes their biogenesis process focuses on presenting known regulation plant development responses biotic abiotic stresses. The also highlights exciting potential future research this field, proposing methods detecting bioinformatic pathway identifying functions.

Язык: Английский

Процитировано

0
Towards understanding cancer dormancy over strategic hitching up mechanisms to technologies DOI Creative Commons
Sumin Yang, Jieun Seo, Jeong‐Hyeon Choi

и другие.

Molecular Cancer, Год журнала: 2025, Номер 24(1)

Опубликована: Фев. 14, 2025

Delving into cancer dormancy has been an inherent task that may drive the lethal recurrence of after primary tumor relief. Cells in quiescence can survive for a short or long term silence, undergo genetic epigenetic changes, and initiate relapse through certain contextual cues. The state be induced by multiple conditions including drug treatment, turn, undergoes life cycle generally occurs dissemination, invasion, intravasation, circulation, immune evasion, extravasation, colonization. Throughout this cascade, cellular machinery governs fate individual cells, largely affected gene regulation. Despite its significance, precise view is yet hampered. Revolutionizing advanced single cell read sequencing analysis methodologies artificial intelligence, most recent stage research tool progress, expected to provide holistic diverse aspects dormancy.

Язык: Английский

Процитировано

0
Human stem cells with in vivo high plasticity generated by cell–cell communication DOI Creative Commons
Shao-wei Li, Xi Chen, Jingxue Xin

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(11)

Опубликована: Март 11, 2025

Stem cells possess inherent properties of self-renewal and differentiation, thus hold significant promise for regenerating damaged tissues or replacing lost cells. Unless their therapeutic effects are solely mediated by paracrine, transplanted stem need to be highly plastic adapt the host tissue environment differentiate into constituent tissue-specific repair. used in current cell-based therapies either have limited differentiation potential pluripotent but must strictly restricted avoid tumorigenicity risk vivo. Here, we describe derivation human adult high-plasticity cells, which call guide-integrated (giaSCs), from interaction blood-derived guide umbilical cord tissue–derived mesenchymal stromal (UC-MSCs). The a cell population derived peripheral blood adults. Unidirectional transfer through nanotube-like structures granular substances recipient UC-MSCs gave rise giaSCs. Topical application giaSCs full-layer excisional wounds wild-type mice led reconstitution skin tissue. Systemically administered migrated reside mouse small intestinal lipopolysaccharides then differentiated epithelial These transplantation experiments demonstrated that vivo high plasticity. Additional vitro data showed low immunogenicity nontumorigenic. indicate offer promising approach therapy.

Язык: Английский

Процитировано

0
Role of long non-coding RNAs in the regulation of ferroptosis in tumors DOI Creative Commons

Ying Ju,

Yuanhao Lv,

Xu Liu

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 21, 2025

Normal cells begin to grow indefinitely and immortalize form tumor after an external stimulus resulting in a genetic mutation. Effective killing of is the basis various cancer therapies. Ferroptosis class cell death types dependent on iron cellular lipid peroxidation. Tumors themselves are iron-dependent, conventional radiotherapy also sensitizes ferroptosis. Increasing sensitivity ferroptosis may be potential therapeutic strategy overcome resistance mechanisms therapy. Long noncoding RNAs (LncRNAs) transcripts more than 200 nucleotides length that regulate gene expression at multiple levels involved biological processes such as differentiation, cycle arrest, maintenance stemness. Recent studies have found lncRNAs through influence or ameliorate chemotherapeutic agents. With continuous maturation nanomaterials technology, it provide new means for treatment by regulating ferroptosis-related inside tumors well increasing Fe 2+ ROS tumors. In this paper, we systematically introduce regulatory mechanism ferroptosis, role immunotherapy application combined with nanomaterials, which provides perspectives

Язык: Английский

Процитировано

0
Psychedelic Drugs in Mental Disorders: Current Clinical Scope and Deep Learning‐Based Advanced Perspectives DOI Creative Commons
Sung‐Hyun Kim, Sumin Yang,

Jeehye Jung

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Mental disorders are a representative type of brain disorder, including anxiety, major depressive depression (MDD), and autism spectrum disorder (ASD), that caused by multiple etiologies, genetic heterogeneity, epigenetic dysregulation, aberrant morphological biochemical conditions. Psychedelic drugs such as psilocybin lysergic acid diethylamide (LSD) have been renewed fascinating treatment options gradually demonstrated potential therapeutic effects in mental disorders. However, the multifaceted conditions psychiatric resulting from individuality, complex interplay, intricate neural circuits impact systemic pharmacology psychedelics, which disturbs integration mechanisms may result dissimilar medicinal efficiency. The precise prescription psychedelic remains unclear, advanced approaches needed to optimize drug development. Here, recent studies demonstrating diverse pharmacological psychedelics reviewed, emerging perspectives on structural function, microbiota-gut-brain axis, transcriptome discussed. Moreover, applicability deep learning is highlighted for development basis big data. These provide insight into interindividual factors enhance discovery precision medicine.

Язык: Английский

Процитировано

0
The power of microRNA regulation—insights into immunity and metabolism DOI Creative Commons
Stefania Oliveto, Nicola Manfrini, Stefano Biffo

и другие.

FEBS Letters, Год журнала: 2025, Номер unknown

Опубликована: Апрель 11, 2025

MicroRNAs (miRNAs) are a prominent class of small non‐coding RNAs that control gene expression. This comprehensive review explores the intricate roles miRNAs in metabolism and immunity, as well emerging field immunometabolism. The core this work delves into functional regulatory capabilities miRNAs, examining their complex influence on glucose lipid metabolism, pivotal shaping T‐cell development function. Specifically, addresses how orchestrate interaction between cellular metabolic processes immune responses, underscoring essential nature these molecules maintaining homeostasis. Finally, we examine role Artificial Intelligence (AI) miRNA research, focusing machine learning techniques revolutionizing identification validation potential biomarkers. By integrating diverse aspects, underscores multifaceted biological significant advancing biomedical research clinical applications.

Язык: Английский

Процитировано

0
Regulatory RNA: from molecular insights to therapeutic frontiers DOI Creative Commons
Tae Soo Kim, Tae-Kyung Kim

Experimental & Molecular Medicine, Год журнала: 2024, Номер 56(6), С. 1233 - 1234

Опубликована: Июнь 14, 2024

Язык: Английский

Процитировано

1
The Emerging Role of IGF2BP2 in Cancer Therapy Resistance: From Molecular Mechanism to Future Potential DOI Open Access

D Li,

Shi‐Qi Hu, Jiarong Ye

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 12150 - 12150

Опубликована: Ноя. 12, 2024

Tumor resistance is one of the primary reasons for cancer treatment failure, significantly limiting options and efficacy therapies. Therefore, overcoming has become a critical factor in improving outcomes. IGF2BP2, as reader m6A methylation, plays pivotal role post-transcriptional regulation RNA through methylation sites. It not only contributes to initiation progression but also key tumor drug resistance. This review provides comprehensive summary mechanisms by which IGF2BP2 therapy resistance, with aim chemotherapy treatment. Advancing research this area crucial developing more effective therapies that could improve quality life patients.

Язык: Английский

Процитировано

1
The RNA Revolution in the Central Molecular Biology Dogma Evolution DOI Open Access
William A. Haseltine, Roberto Patarca

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12695 - 12695

Опубликована: Ноя. 26, 2024

Human genome projects in the 1990s identified about 20,000 protein-coding sequences. We are now RNA revolution, propelled by realization that genes determine phenotype beyond foundational central molecular biology dogma, stating inherited linear pieces of DNA transcribed to RNAs and translated into proteins. Crucially, over 95% genome, initially considered junk between genes, encodes essential, functionally diverse non-protein-coding RNAs, raising gene count at least one order magnitude. Most phenotype-determining changes regulatory areas control can directly or indirectly phenotypes regulating protein function, transferring information within organisms, generating DNA. also exhibit high structural, functional, biomolecular interaction plasticity modified via editing, methylation, glycosylation, other mechanisms, which bestow them with intra- extracellular functions without altering underlying is, therefore, currently primary determinant cellular populational functional diversity, disease-linked structural variations, cell function regulation. As demonstrated RNA-based coronavirus vaccines' success, technology is transforming medicine, agriculture, industry, as did advent recombinant 1980s.

Язык: Английский

Процитировано

0