bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 28, 2024
Abstract
The
combination
of
meglumine
antimoniate
and
allopurinol
is
considered
one
the
most
effective
treatments
for
canine
leishmaniosis
caused
by
Leishmania
infantum
.
This
study
investigated
effects
this
treatment
on
gut
microbiome
10
dogs
from
Spain,
Portugal,
Italy
via
fecal
shotgun
metagenomic
sequencing
over
six
months.
Dogs
were
sampled
at
baseline
(BL)
(M1)
(M6)
months
post-treatment.
-infected
dominated
Prevotella
,
Collinsella
Bacteroides
Blautia
with
individual
variability
being
primary
determinant
composition.
No
significant
changes
in
alpha
diversity
(Shannon
index,
gene
number)
or
beta
(Bray-Curtis
dissimilarity,
UniFrac
distance)
detected
between
pre-
post-treatment
time
points,
suggesting
that
does
not
disrupt
microbiota.
Minor
trends
taxonomic
shifts
noted,
slight
increases
Bifidobacterium
pseudocantenulatum
tanakaei
Slackia
piriformis
after
treatment,
but
these
statistically
correction
multiple
testing.
Linear
discriminant
analysis
multivariable
modeling
confirmed
microbial
community
structure
was
resilient
to
effects.
Individual-specific
differences
accounted
52%
observed
variability,
underscoring
personalized
nature
microbiota
dogs.
Importantly,
no
adverse
disruptions
detected,
even
prolonged
use.
highlights
robustness
during
antileishmanial
therapy
use
without
compromising
health.
Further
studies
larger
cohorts
are
recommended
confirm
findings
explore
functional
roles
modulating
immune
responses
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 15, 2025
With
growing
recognition
of
the
pivotal
role
gut
microbiota
in
human
health,
probiotics
have
gained
widespread
attention
for
their
potential
to
restore
microbial
homeostasis.
However,
a
critical
challenge
persists:
limited
colonization
efficiency
among
most
probiotic
strains
compromises
therapeutic
efficacy.
This
overview
synthesizes
ecological
principles
with
cutting-edge
microbiome
research
elucidate
dynamic
interplay
between
dietary
components
and
within
intestinal
niche.
systematically
analyzes:
(1)
stage-specific
mechanisms
spanning
introduction,
establishment,
proliferation;
(2)
nutrient-driven
modulation
composition
function;
(3)
dual
common
patterns
as
both
facilitators
disruptors
persistence.
Notably,
this
identifies
key
strategies,
including
precision
delivery
prebiotic
fibers
polyphenol-microbiota
crosstalk,
that
enhance
niche
adaptation
through
pH
optimization,
adhesion
potentiation,
competitive
exclusion
pathogens.
Furthermore,
critically
evaluates
current
limitations
research,
particularly
strain-specific
variability
methodological
constraints
simulating
host-microbe-diet
tripartite
interactions.
To
bridge
these
gaps,
proposes
an
interdisciplinary
framework
integrating
omics-driven
strain
selection,
engineered
systems,
personalized
nutrition
models.
Collectively,
work
advances
mechanistic
understanding
diet-microbiota
interactions
while
providing
actionable
insights
developing
targeted
therapies
evidence-based
interventions
optimize
ecosystem
resilience.
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2025,
Номер
15
Опубликована: Апрель 17, 2025
Introduction
The
rising
use
of
dental
implants
is
accompanied
by
an
expected
increase
in
peri-implant
diseases,
particularly
peri-implantitis
(PI),
which
poses
a
significant
threat
to
implant
success
and
necessitates
thorough
understanding
its
pathogenesis
for
effective
management.
Methods
To
gain
deeper
insights
into
the
role
impact
microbiome
progression
PI,
we
analyzed
100
samples
saliva
subgingival
biofilm
from
40
participants
with
healthy
(HI
group)
or
co-occurrence
diagnosed
PI-affected
(PI
using
shotgun
metagenomic
sequencing.
We
identified
most
discriminative
species
distinguishing
diseased
study
groups
through
log
ratios
differential
ranking
analyses.
Results
discussion
Mogibacterium
timidum
,
Schaalia
cardiffensis
Parvimonas
micra
Filifactor
alocis
Porphyromonas
endodontalis
gingivalis
Olsenella
uli
were
associated
biofilm.
In
contrast,
Neisseria
sp
oral
taxon
014,
Haemophilus
parainfluenzae
Actinomyces
naeslundii
Rothia
mucilaginosa
aeria
more
prevalent
Functional
pathways
such
as
arginine
polyamine
biosynthesis,
including
putrescine
citrulline
showed
stronger
correlations
implants.
health
was
characterized
predominance
involved
purine
pyrimidine
deoxyribonucleotide
de
novo
glucose
glucose-1-phosphate
degradation,
tetrapyrrole
biosynthesis.
Our
findings
reveal
that
PI-free
cavities
differ
significantly
microbial
composition
functional
compared
co-occurring
implants,
closely
resemble
PI-associated
profiles.
This
pattern
extended
salivary
samples,
where
biomarkers
follow
similar
trends.
Microorganisms,
Год журнала:
2024,
Номер
12(11), С. 2341 - 2341
Опубликована: Ноя. 16, 2024
Recent
advances
have
highlighted
the
gut
microbiota
as
a
significant
contributor
to
development
and
progression
of
atherosclerosis,
which
is
an
inflammatory
cardiovascular
disease
(CVD)
characterized
by
plaque
buildup
within
arterial
walls.
The
microbiota,
consisting
diverse
collection
microorganisms,
impacts
host's
metabolism,
immune
responses,
lipid
processing,
all
contribute
atherosclerosis.
This
review
explores
complex
mechanisms
through
dysbiosis
promotes
atherogenesis.
We
emphasize
potential
integrating
modulation
with
traditional
care,
offering
holistic
approach
managing
Important
pathways
involve
translocation
microbial
components,
metabolism
metabolites
such
trimethylamine-N-oxide
(TMAO),
production
short-chain
fatty
acids
(SCFAs)
that
influence
vascular
health.
Studies
reveal
distinct
profiles
in
atherosclerosis
patients,
increased
pathogenic
bacteria
(
Journal of Preventive Diagnostic and Treatment Strategies in Medicine,
Год журнала:
2024,
Номер
3(4), С. 284 - 295
Опубликована: Окт. 1, 2024
Abstract
BACKGROUND:
Metagenomics
plays
a
vital
role
in
studying
microbial
genomes
and
their
implications
for
human
health.
The
gut
microbiome
begins
to
develop
at
birth
is
shaped
by
factors
such
as
maternal
nutrition
antibiotic
exposure.
Disruptions
this
community
can
impact
an
infant’s
immune
system,
metabolism,
susceptibility
various
diseases.
This
research
aims
predict
the
taxonomic
functional
profiling
of
metagenome
mothers
infants
exposed
antibiotics.
study
utilized
metagenomic
samples
retrieved
from
European
nucleotide
archive
database
under
accession
number
PRJNA698986.
Four
were
analyzed,
including
two
corresponding
infant
samples.
design
focuses
on
comprehensive
assessment
diversity
pathways
using
advanced
bioinformatics
detailed
classification
analysis
data.
MATERIALS
AND
METHODS:
Taxonomic
was
conducted
MetaPhlAn2
extract
analyze
structure.
Functional
performed
with
HUMAnN2
evaluate
functions.
RESULTS:
Metagenomic
revealed
significant
differences
communities
between
mothers.
infants’
initial
exhibited
diverse
presence
both
bacteria
viruses,
whereas
predominantly
contained
bacteria,
minor
representation
archaea.
CONCLUSIONS:
highlights
exposure
infants,
illustrating
distinct
composition
compared
findings
emphasize
need
further
understand
roles
these
health
disease.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 11, 2024
Abstract
Understanding
the
human
gut
microbiome
requires
comprehensive
genomic
catalogs,
yet
many
existing
ones
lack
geographic
diversity
and
include
numerous
medium-quality
metagenome-assembled
genomes
(MAGs)
missing
up
to
50%
of
regions,
potentially
leading
inaccurate
functional
insights
into
microbiome.
To
address
these
limitations,
we
developed
an
enhanced
Human
Reference
Gut
Microbiome
(HRGM2),
a
catalog
near-complete
(NC)
MAGs
(completeness
≥90%
contamination
≤5%)
isolate
(collectively
‘NC
genomes’)
with
extensive
sampling.
HRGM2
comprises
155,211
non-redundant
NC
from
4,824
prokaryotic
species
across
41
countries,
representing
66%
increase
in
genome
count
boost
over
genome-based
Unified
Gastrointestinal
Genome
catalog.
Extensive
sampling
Africa
revealed
previously
undetectable
Africa-associated
phyla,
highlighting
microbial
links
between
humans
environment.
Given
high
complexity
microbiota,
DNA-based
profiling
using
outperforms
marker-based
accuracy.
Profiling
protein
annotations
provides
reliable
Notably,
exclusive
use
genomes,
enhances
assessments
microbiota
metabolic
capacities
supports
genome-scale
models
(GEMs)
entire
microbiota.
These
findings
offer
novel
perspectives
on
microbiome,
achievable
only
through
high-quality
cataloging.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 28, 2024
Abstract
The
combination
of
meglumine
antimoniate
and
allopurinol
is
considered
one
the
most
effective
treatments
for
canine
leishmaniosis
caused
by
Leishmania
infantum
.
This
study
investigated
effects
this
treatment
on
gut
microbiome
10
dogs
from
Spain,
Portugal,
Italy
via
fecal
shotgun
metagenomic
sequencing
over
six
months.
Dogs
were
sampled
at
baseline
(BL)
(M1)
(M6)
months
post-treatment.
-infected
dominated
Prevotella
,
Collinsella
Bacteroides
Blautia
with
individual
variability
being
primary
determinant
composition.
No
significant
changes
in
alpha
diversity
(Shannon
index,
gene
number)
or
beta
(Bray-Curtis
dissimilarity,
UniFrac
distance)
detected
between
pre-
post-treatment
time
points,
suggesting
that
does
not
disrupt
microbiota.
Minor
trends
taxonomic
shifts
noted,
slight
increases
Bifidobacterium
pseudocantenulatum
tanakaei
Slackia
piriformis
after
treatment,
but
these
statistically
correction
multiple
testing.
Linear
discriminant
analysis
multivariable
modeling
confirmed
microbial
community
structure
was
resilient
to
effects.
Individual-specific
differences
accounted
52%
observed
variability,
underscoring
personalized
nature
microbiota
dogs.
Importantly,
no
adverse
disruptions
detected,
even
prolonged
use.
highlights
robustness
during
antileishmanial
therapy
use
without
compromising
health.
Further
studies
larger
cohorts
are
recommended
confirm
findings
explore
functional
roles
modulating
immune
responses
