Base editors in zebrafish: a new era for functional genomics and disease modeling DOI Creative Commons
Yuwen Liu, Chao Li,

Yating Qiu

и другие.

Frontiers in Genome Editing, Год журнала: 2025, Номер 7

Опубликована: Май 21, 2025

Base editing has revolutionized genome engineering by enabling precise single-nucleotide modifications without inducing double-strand breaks. As a powerful and efficient gene-editing tool, base editors (BEs) have been widely applied in various model organisms, including zebrafish ( Danio rerio ), to facilitate functional genomic studies disease modeling. Zebrafish, with its genetic similarity humans rapid development, provides an excellent platform for testing optimizing emerging technologies. This review comprehensively explores the advancements of cytosine adenine zebrafish, highlighting recent developments that enhance efficiency, specificity, scope. We discuss novel editor variants tailored applications, improvements delivery strategies, methodologies minimize off-target effects. Furthermore, we compare other precision genome-editing technologies, such as prime homology-directed repair, underscore advantages achieving targeted mutations high fidelity. By evaluating expanding role this valuable insights into potential translational research, modeling, future therapeutic applications.

Язык: Английский

Base editors in zebrafish: a new era for functional genomics and disease modeling DOI Creative Commons
Yuwen Liu, Chao Li,

Yating Qiu

и другие.

Frontiers in Genome Editing, Год журнала: 2025, Номер 7

Опубликована: Май 21, 2025

Base editing has revolutionized genome engineering by enabling precise single-nucleotide modifications without inducing double-strand breaks. As a powerful and efficient gene-editing tool, base editors (BEs) have been widely applied in various model organisms, including zebrafish ( Danio rerio ), to facilitate functional genomic studies disease modeling. Zebrafish, with its genetic similarity humans rapid development, provides an excellent platform for testing optimizing emerging technologies. This review comprehensively explores the advancements of cytosine adenine zebrafish, highlighting recent developments that enhance efficiency, specificity, scope. We discuss novel editor variants tailored applications, improvements delivery strategies, methodologies minimize off-target effects. Furthermore, we compare other precision genome-editing technologies, such as prime homology-directed repair, underscore advantages achieving targeted mutations high fidelity. By evaluating expanding role this valuable insights into potential translational research, modeling, future therapeutic applications.

Язык: Английский

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