Binge Eating and Obesity Differentially Alter the Mesolimbic Endocannabinoid System in Rats

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 1240 - 1240

Опубликована: Янв. 31, 2025

Binge eating disorder (BED) is characterized by the rapid overconsumption of palatable food in a short amount time, often leading to obesity. The endocannabinoid system (ECS), involved intake, highly expressed reward-related brain regions and both obesity BED. This study investigated differences ECS expression between these conditions using male Wistar rats exposed specific regimen over six weeks: non-access group (NA) with standard diet, continuous access (CA) free-choice high-fat high-sugar (fcHFHS) diet modeling obesity, an intermittent (IA) fcHFHS Food intake was measured, tissues from nucleus accumbens (NAc), dorsal striatum (DS), ventral tegmental area (VTA), rostromedial (RMTg) were analyzed for qPCR mass spectrometry. We identified differential across groups, variations depending on region (striatal or mesencephalic). Correlation analyses revealed dysregulations dependent type (fat sucrose) quantity consumed. Comparative network analysis co-regulation patterns ECS-related genes signatures associated each pattern, highlighting RMTg as key future research behavior.

Язык: Английский

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Coadministration antagonist dopamine receptor D4 with CB2 receptor agonist decreases binge-like intake of palatable food in mice

Frontiers in Behavioral Neuroscience, Год журнала: 2025, Номер 19

Опубликована: Апрель 29, 2025

Food intake is regulated by two systems: homeostatic and hedonic. An imbalance between these systems can induce overconsumption, such as binge eating disorder (BED), associated with dysregulation of the dopamine reward system. The cannabinoid type 2 receptor (CB2R) has been identified in neurons may play an important role motivated behaviors, including food intake. Nevertheless, interaction D4 (DRD4) CB2R binge-like not yet identified. Therefore, present study aims to evaluate effects intraperitoneal administration DRD4 antagonist (L-745870), well coadministration either agonist (HU308) or (AM630), on palatable (PF) adult male mice. We used 34 C57BL6/J All animals were housed individually had ad libitum access standard diet (SD) water. To intake, 1 h PF during 12 baseline test (BET) sessions. Mice then randomly assigned following treatment groups: 1) vehicle; 2) L-745870; 3) L-745870-HU308, 4) L-745870+AM630 be evaluated under effect treatments for three additionally BET Our results show that reduced PF, a induced even more pronounced reduction These findings suggest dopaminergic endocannabinoid modulation mice, where activation participates modulating pathways reducing behavior.

Язык: Английский

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Brain coding of maternal motivation: the role of the tail of the ventral tegmental area

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Май 12, 2025

Motherhood entails brain and behavioral changes associated with increased motivation for pups, ensuring their correct development survival. Dopamine systems play a crucial role in motivated behaviors, although the exact neurobiological mechanisms underlying maternal behavior remain unknown. The tail of ventral tegmental area (tVTA) or rostromedial nucleus (RMTg) is control center dopamine involved avoidance prediction error, among other processes. In present study, we explored its possible contribution rats. To do so, analyzed behavior, as well expression cFos several regions (tVTA/RMTg, anterior posterior VTA, shell core ACb, mPFC, LHb, MePD, MPO) virgin dam rats response to pups (Virgin-P, DamP) pup-predicting cues (absence pups) (Virgin-NP, Dam-NP). Overall, our results reveal that was only displayed by lactating females, whereas virgins did not display sensitization experimental conditions. Regarding activity specific regions, show induce higher tVTA/RMTg pup-deprived dams compared non-pup deprived suggesting reward error. By contrast, pup exposure deprivation elicit huge differences on recruitment social-related females. Finally, correlation analysis mainly highlights positive correlations pup-exposed females scarce

Язык: Английский

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Reduced GABA transmission onto ventral tegmental area dopamine neurons underlies vulnerability to a mouse model of Anorexia Nervosa

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 13, 2024

Anorexia nervosa (AN) has the highest mortality among psychiatric diseases. Hyperactivity is a persistent symptom and alteration of mesolimbic dopamine transmission been linked to development maintenance disease hyperactivity. However, whether local neurocircuit plasticity causally involved remains unclear. Especially role GABA control over neurons, powerful regulator system, in an AN context unresolved. We hypothesize that combining caloric restriction with exercise alters via disinhibition that, turn, facilitates expression maladaptive behaviors such as Therefore, we characterized impact activity-based anorexia (ABA) model on reward system using ex-vivo electrophysiology coupled optogenetic manipulations. Ventral tegmental area (VTA_DA) neurons displayed higher firing frequency ABA-exposed animals compared mice. This coincided reduced GABAergic VTA_DA at least part attributable decreased excitability VTA (VTA_GABA) neurons. Restoring VTA_GABA chemogenetic activation rescued mice from starvation, by decreasing running wheel activity. In summary, found anorexic state leads dysregulation reinforces uncovered new mechanism disturbed animals, identifying hitherto unknown neuron output.

Язык: Английский

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