Degron-modified Cas12a enhances single-cell CRISPR screening DOI Creative Commons
Valentina Snetkova,

Carolina Galan,

Romain Lopez

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 8, 2024

Abstract Single-cell CRISPR (Perturb-seq) screens have primarily relied on Cas9 whereas Cas12a, despite its unique effectiveness for multiplex guide expression, remains underexplored. This may be due to Cas12a’s RNA array (pre-crRNA) self-processing activity and the subsequent challenges associated with pre-crRNA sequence recovery. By developing modified expression vectors a degron-based Cas12a system, we overcome constraint, allowing accurate detection of pre-crRNAs at single-cell level, thus greatly expanding possibilities future Perturb-seq efforts.

Язык: Английский

Association between NAT10 gene rs8187 G > A polymorphism and Wilms tumor susceptibility in Chinese Han children: a five-center case-control study DOI Creative Commons
Changmi Deng, Jinhong Zhu, Fei Duan

и другие.

BMC Cancer, Год журнала: 2025, Номер 25(1)

Опубликована: Март 17, 2025

Wilms tumor, a prevalent pediatric kidney cancer, has been extensively studied to elucidate its genetic mechanisms. NAT10 (N-acetyltransferase 10) is gene encoding acetyltransferase, which involved in various cellular processes, including RNA modification, DNA repair, and protein acetylation. The oncogenic role of cancer garnered significant attention. However, research on variants their associations with nascent. This study investigated the link between tumor risk via case‒control design genomic from 414 patients 1199 controls. Genotyping was performed TaqMan method, logistic regression statistical analysis conducted identify associations, followed by extra minimize false positive results. Our findings revealed that rs8187 G > A polymorphism significantly correlated decreased developing (GA vs. GG, adjusted odds ratio (AOR) = 0.60, 95% confidence interval (CI) 0.46-0.77, P < 0.0001; GA/AA AOR 0.74, CI 0.59-0.93, 0.011). Stratified analyses further association children aged 18 months or under subgroups stage II, IV, combined I + II tumors. These results highlight potential as markers for susceptibility. clarifies basis susceptibility highlights early detection assessment.

Язык: Английский

Процитировано

0
Targeting NAT10 attenuates homologous recombination via destabilizing DNA:RNA hybrids and overcomes PARP inhibitor resistance in cancers DOI
Zhu Xu, Mingming Zhu,

Longpo Geng

и другие.

Drug Resistance Updates, Год журнала: 2025, Номер unknown, С. 101241 - 101241

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Improvement of MASLD and MASH by suppression of hepatic N-Acetyltransferase 10 DOI Creative Commons
Yanying Yang, Jie Lu, Yuejun Liu

и другие.

Molecular Metabolism, Год журнала: 2024, Номер 89, С. 102030 - 102030

Опубликована: Сен. 17, 2024

Metabolic dysfunction-associated steatotic liver disease (MASLD) and metabolic steatohepatitis (MASH) are characterized by excessive triglyceride accumulation in the liver. However, due to an incomplete understanding of its pathogenesis, more efforts needed identify specific effective treatments. N4-acetylcytidine (ac4C) is a newly discovered RNA modification regulate mRNA. N-acetyltransferase 10 (NAT10) has not been fully explored MASLD MASH.

Язык: Английский

Процитировано

2
The role and mechanism of NAT10‐mediated ac4C modification in tumor development and progression DOI Creative Commons

Zhuoran Gu,

Libin Zou,

Xinjian Pan

и другие.

MedComm, Год журнала: 2024, Номер 5(12)

Опубликована: Дек. 1, 2024

Abstract RNA modification has emerged as a crucial area of research in epigenetics, significantly influencing tumor biology by regulating metabolism. N‐acetyltransferase 10 (NAT10)‐mediated N4‐acetylcytidine (ac4C) modification, the sole known acetylation eukaryotic RNA, influences cancer pathogenesis and progression. NAT10 is only writer ac4C catalyzes acetyl transfer on targeted helps to improve stability translational efficiency ac4C‐modified RNA. highly expressed associated with poor prognosis pan‐cancers. Based its molecular mechanism biological functions, central factor tumorigenesis, progression, drug resistance, immune escape. Despite increasing focus ac4C, specific regulatory mechanisms remain elusive. The present review thoroughly analyzes current knowledge NAT10‐mediated cancer, highlighting broad influence gene expression biology. This also summarizes limitations perspectives identify new therapeutic targets advance treatment strategies.

Язык: Английский

Процитировано

0
Degron-modified Cas12a enhances single-cell CRISPR screening DOI Creative Commons
Valentina Snetkova,

Carolina Galan,

Romain Lopez

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 8, 2024

Abstract Single-cell CRISPR (Perturb-seq) screens have primarily relied on Cas9 whereas Cas12a, despite its unique effectiveness for multiplex guide expression, remains underexplored. This may be due to Cas12a’s RNA array (pre-crRNA) self-processing activity and the subsequent challenges associated with pre-crRNA sequence recovery. By developing modified expression vectors a degron-based Cas12a system, we overcome constraint, allowing accurate detection of pre-crRNAs at single-cell level, thus greatly expanding possibilities future Perturb-seq efforts.

Язык: Английский

Процитировано

0