Folic Acid Promotes Peripheral Nerve Injury Repair via Regulating DNM3-AKT Pathway Through Mediating Methionine Cycle Metabolism DOI Creative Commons

Weibo Kang,

Yanli Zhang,

Wei Cui

и другие.

NeuroMolecular Medicine, Год журнала: 2025, Номер 27(1)

Опубликована: Март 31, 2025

Emerging evidence suggests that folic acid (FA) supports nerve repair, but its beneficial effects in peripheral injury (PNI) remains unclear. This study aims to investigate protective of FA against PNI and the underlying molecular mechanisms. High-performance liquid chromatography-tandem mass spectrometry was utilized for precise quantification metabolites. A sciatic crush model established rats, followed by assessments cell proliferation, apoptosis, motor function using CCK-8 assays, flow cytometry, balance beam test, respectively. Neuromorphological observations, electromyography, ELISA were conducted evaluate structural, electrophysiological, biochemical parameters. In vitro, restored methionine cycle Schwann cells neurons disrupted enzyme inhibition, improving viability, reducing preserving cellular structure. vivo, supplementation S-adenosylmethionine homocysteine levels a metabolism disorder enhanced function, neural morphology, neuron survival, electrophysiological recovery after PNI. Epigenetic analyses revealed modulated DNA methylation histone modifications DNM3 promoter, influencing gene expression. Furthermore, facilitated repair via DNM3-AKT pathway, regulating autophagy, oxidative stress-related enzymes. These findings highlight FA's potential promoting through metabolic epigenetic

Язык: Английский

Bridging epigenomics and tumor immunometabolism: molecular mechanisms and therapeutic implications DOI Creative Commons
Xiaowen Xie, Weici Liu, Zhiyuan Yuan

и другие.

Molecular Cancer, Год журнала: 2025, Номер 24(1)

Опубликована: Март 8, 2025

Epigenomic modifications—such as DNA methylation, histone acetylation, and methylation—and their implications in tumorigenesis, progression, treatment have emerged a pivotal field cancer research. Tumors undergo metabolic reprogramming to sustain proliferation metastasis nutrient-deficient conditions, while suppressing anti-tumor immunity the tumor microenvironment (TME). Concurrently, immune cells within immunosuppressive TME adaptations, leading alterations function. The complicated interplay between metabolites epigenomic modulation has spotlighted significance of regulation immunometabolism. In this review, characteristics modification associated with tumors are systematically summarized alongside regulatory roles Classical emerging approaches delineated broaden boundaries research on crosstalk immunometabolism epigenomics. Furthermore, we discuss potential therapeutic strategies that target modulate modifications, highlighting burgeoning synergy therapies immunotherapy promising avenue for treatment.

Язык: Английский

Процитировано

1
SFP6 fluorescent probes for imaging SAM dynamics in living cells DOI
Shuhui Zhang,

Jinghui Li,

Gengsheng Cao

и другие.

Microchimica Acta, Год журнала: 2025, Номер 192(3)

Опубликована: Фев. 21, 2025

Язык: Английский

Процитировано

0
Folic Acid Promotes Peripheral Nerve Injury Repair via Regulating DNM3-AKT Pathway Through Mediating Methionine Cycle Metabolism DOI Creative Commons

Weibo Kang,

Yanli Zhang,

Wei Cui

и другие.

NeuroMolecular Medicine, Год журнала: 2025, Номер 27(1)

Опубликована: Март 31, 2025

Emerging evidence suggests that folic acid (FA) supports nerve repair, but its beneficial effects in peripheral injury (PNI) remains unclear. This study aims to investigate protective of FA against PNI and the underlying molecular mechanisms. High-performance liquid chromatography-tandem mass spectrometry was utilized for precise quantification metabolites. A sciatic crush model established rats, followed by assessments cell proliferation, apoptosis, motor function using CCK-8 assays, flow cytometry, balance beam test, respectively. Neuromorphological observations, electromyography, ELISA were conducted evaluate structural, electrophysiological, biochemical parameters. In vitro, restored methionine cycle Schwann cells neurons disrupted enzyme inhibition, improving viability, reducing preserving cellular structure. vivo, supplementation S-adenosylmethionine homocysteine levels a metabolism disorder enhanced function, neural morphology, neuron survival, electrophysiological recovery after PNI. Epigenetic analyses revealed modulated DNA methylation histone modifications DNM3 promoter, influencing gene expression. Furthermore, facilitated repair via DNM3-AKT pathway, regulating autophagy, oxidative stress-related enzymes. These findings highlight FA's potential promoting through metabolic epigenetic

Язык: Английский

Процитировано

0