Methionine-driven YTHDF1 expression facilitates bladder cancer progression by attenuating RIG-I-modulated immune responses and enhancing the eIF5B-PD-L1 axis DOI Creative Commons
Anze Yu, Liangmin Fu,

Lanyu Jing

и другие.

Cell Death and Differentiation, Год журнала: 2024, Номер unknown

Опубликована: Дек. 13, 2024

Abstract The impact of amino acids on tumor immunotherapy is gradually being uncovered. In this study, we screened various essential and non-essential found that methionine enhances mRNA methylation reduced the activation Type I interferon pathway in bladder cancer. Through RNA sequencing, point mutations, MB49 mouse models, single-cell demonstrated high levels elevate expression m 6 A reader YTHDF1, promoting degradation RIG-I, thereby inhibiting RIG-I/MAVS-mediated IFN-I reducing efficacy immunotherapy. Additionally, immunoprecipitation mass spectrometry revealed YTHDF1 binds to eukaryotic translation initiation factor eIF5B, which acts PD-L1 enhance its promote immune evasion. By intravesical administration oncolytic bacteria VNP20009, effectively depleted locally, significantly prolonging survival enhancing cell infiltration differentiation within tumors. Multiplex immunofluorescence assays cancer patients confirmed our findings. Our research elucidates two mechanisms by inhibits proposes a targeted depletion strategy advances while minimizing nutritional patients.

Язык: Английский

Delivering living medicines with biomaterials DOI
Tetsuhiro Harimoto, Wei‐Hung Jung, David Mooney

и другие.

Nature Reviews Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

Язык: Английский

Процитировано

3
Dual‐Engineered Macrophage‐Microbe Encapsulation for Metastasis Immunotherapy DOI
Leyang Wu,

Liyuan Qiao,

Shu‐Hui Zhang

и другие.

Advanced Materials, Год журнала: 2024, Номер unknown

Опубликована: Июль 18, 2024

Abstract Lung metastases are the leading cause of death among cancer patients. The challenges inefficient drug delivery, compounded by a robust immunosuppressive microenvironment, make effective treatment difficult. Here, an innovative dual‐engineered macrophage‐microbe encapsulation (Du‐EMME) therapy is developed that integrates modified macrophages and engineered antitumor bacteria. These macrophages, termed R‐GEM cells, designed to express RGD peptides on extracellular membranes, enhancing their tumor cell binding intratumor enrichment. cells cocultured with attenuated Salmonella typhimurium VNP20009, producing (R‐GEM/VNP cells). intracellular bacteria maintain bioactivity for more than 24 h, released from R‐GEM/VNP within continue exert bacteria‐mediated effects. This further supported macrophage‐based chemotaxis camouflage, which enhance intratumoral enrichment biocompatibility Additionally, loaded IFNγ‐secreting strains (VNP‐IFNγ) form R‐GEM/VNP‐IFNγ cells. Treatment these effectively halts lung metastatic progression in three mouse models (breast cancer, melanoma, colorectal cancer). vigorously activate suppressing tumor‐promoting M2‐type MDSCs, Tregs, tumor‐antagonizing M1‐type mature DCs, Teffs. Du‐EMME offers promising strategy targeted enhanced immunity treating metastases.

Язык: Английский

Процитировано

7
Bacterial-Mediated In Situ Engineering of Tumour-Associated Macrophages for Cancer Immunotherapy DOI Open Access

Gabriela Christina Kuhl,

Mark Tangney

Cancers, Год журнала: 2025, Номер 17(5), С. 723 - 723

Опубликована: Фев. 20, 2025

Background/Objectives: Tumour-associated macrophages (TAMs) are critical components of the tumour microenvironment (TME), significantly influencing cancer progression and treatment resistance. This review aims to explore innovative use engineered bacteria reprogram TAMs, enhancing their anti-tumour functions improving therapeutic outcomes. Methods: We conducted a systematic following predefined protocol. Multiple databases were searched identify relevant studies on phenotypic plasticity, for reprogramming. Inclusion exclusion criteria applied select studies, data extracted using standardised forms. Data synthesis was performed summarise findings, focusing mechanisms benefits non-pathogenic modify TAMs. Results: The summarises findings that can selectively target promoting shift from tumour-promoting M2 phenotype tumour-fighting M1 phenotype. reprogramming enhances pro-inflammatory responses activity within TME. Evidence various indicates significant regression improved immune bacterial therapy. Conclusions: Reprogramming TAMs presents promising strategy approach leverages natural targeting abilities directly tumour, potentially patient outcomes offering new insights into immune-based treatments. Further research is needed optimise these methods assess clinical applicability.

Язык: Английский

Процитировано

1
Camouflaging Attenuated Salmonella by Detachable Metal Polyphenol Nanoshells for Enhanced Bacteria-Based Cancer Immunotherapy DOI
Bin Zhang, Rui‐Mei Jin,

Xiao‐Ting Xie

и другие.

ACS Materials Letters, Год журнала: 2025, Номер unknown, С. 1593 - 1602

Опубликована: Март 26, 2025

Язык: Английский

Процитировано

1
Programmable Bacteria with Dynamic Virulence Modulation System for Precision Antitumor Immunity DOI Creative Commons
Leyang Wu, Lin Li,

Liyuan Qiao

и другие.

Advanced Science, Год журнала: 2024, Номер unknown

Опубликована: Июль 26, 2024

Engineered bacteria-mediated antitumor approaches have been proposed as promising immunotherapies for cancer. However, the off-target bacterial toxicity narrows therapeutic window. Living microbes will benefit from their controllable immunogenicity within tumors safer applications. In this study, a genetically encoded microbial activation strategy is reported that uses tunable and dynamic expression of surface extracellular polysaccharides to improve biocompatibility while retaining efficacy. Based on screening genes associated with Salmonella survival in macrophages, novel attenuated chassis strain AIS (htrA gene-deficient) highly enriched after administration rapidly cleared normal organs are reported. Subsequently, an engineered strain, AISI-H, constructed based optimized quorum-sensing regulatory system. The AISI-H can achieve recovery tumor-specific virulence through HTRA-RCSA axis-based synthetic gene circuit-mediated increase polysaccharide content. These strains act "off" avoid unwanted immune "on" precise tumor suppression mice. shows significant inhibition potent anticancer immunity melanoma mouse model. exhibits excellent biocompatibility. This regulation expands applications microbe-based therapeutics.

Язык: Английский

Процитировано

4
Living Therapeutics: Precision Diagnosis and Therapy with Engineered Bacteria DOI

Ruyan Xie,

Duoyang Fan, Cheng Xiang

и другие.

Biomaterials, Год журнала: 2025, Номер 321, С. 123342 - 123342

Опубликована: Апрель 15, 2025

Язык: Английский

Процитировано

0
Paving the way for bacteria-based drug delivery: biohybrid microrobots emerging from microrobotics and synthetic biology DOI Creative Commons
Elena Totter,

Emilie von Einsiedel,

Lisa Regazzoni

и другие.

Advanced Drug Delivery Reviews, Год журнала: 2025, Номер unknown, С. 115577 - 115577

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
The Role of Bacteria in Central Nervous System Tumors: Opportunities and Challenges DOI Creative Commons
Rui Zhang, Xueying Li, Si Zhang

и другие.

Microorganisms, Год журнала: 2024, Номер 12(6), С. 1053 - 1053

Опубликована: Май 23, 2024

Tumors of the central nervous system (CNS) are severe and refractory diseases with poor prognosis, especially for patients malignant glioblastoma brain metastases. Currently, numerous studies have explored potential role bacteria intestinal flora in tumor development treatment. Bacteria can penetrate blood-brain barrier (BBB), targeting hypoxic microenvironment at core tumors, thereby eliminating tumors activating both innate adaptive immune responses, rendering them promising therapeutic agents CNS tumors. In addition, engineered derivatives, such as bacterial membrane proteins spores, also be used good candidate carriers targeted drug delivery. Moreover, regulate metabolism influence through "gut-brain axis". Therefore, anti-tumor therapy, delivery, intervention provide modalities treatment this paper, we performed a comprehensive review mechanisms practices therapy discussed future research directions field.

Язык: Английский

Процитировано

2
Engineered Bacteria: Strategies and Applications in Cancer Immunotherapy DOI Creative Commons
Shuhao Zhang, Rui Li, Yunxue Xu

и другие.

Fundamental Research, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

2
Application of nanomaterials in heart transplantation: a narrative review DOI Open Access

Huaiyu Jiang,

Qiang Zhao,

Xiaofeng Ye

и другие.

Journal of Thoracic Disease, Год журнала: 2024, Номер 16(5), С. 3389 - 3405

Опубликована: Май 1, 2024

Heart transplantation (HT) is a therapeutic option for end-stage heart disease. Still, it faces many challenges, especially the shortage of donor sources and poor durability grafts, which are two critical issues. In this review, we generalize application existing nanomedicine technologies in management as well prevention diagnosis post-transplantation complications, also including current preclinical studies nanomaterials cardiac tissue engineering gene-editing xeno-donor grafts. Finally, discuss remaining problems future directions field HT.

Язык: Английский

Процитировано

1