Cell reprogramming: methods, mechanisms and applications

Cell Regeneration, Год журнала: 2025, Номер 14(1)

Опубликована: Март 27, 2025

Abstract Cell reprogramming represents a powerful approach to achieve the conversion cells of one type into another interest, which has substantially changed landscape in field developmental biology, regenerative medicine, disease modeling, drug discovery and cancer immunotherapy. is complex ordered process that involves coordination transcriptional, epigenetic, translational metabolic changes. Over past two decades, range questions regarding facilitators/barriers, trajectories, mechanisms cell have been extensively investigated. This review summarizes recent advances mediated by transcription factors or chemical molecules, followed elaborating on important roles biophysical cues reprogramming. Additionally, this will detail our current understanding govern reprogramming, including involvement recently discovered biomolecular condensates. Finally, discusses broad applications future directions development, regenerative/rejuvenation therapy,

Язык: Английский

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Bone Defect Treatment in Regenerative Medicine: Exploring Natural and Synthetic Bone Substitutes

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3085 - 3085

Опубликована: Март 27, 2025

In recent years, the management of bone defects in regenerative medicine and orthopedic surgery has been subject extensive research efforts. The complexity fractures loss arising from trauma, degenerative conditions, or congenital disorders necessitates innovative therapeutic strategies to promote effective healing. Although tissue exhibits an intrinsic capacity, critical-sized can severely compromise this process, often requiring grafts substitutes. Tissue engineering approaches within have introduced novel possibilities for addressing nonunions challenging refractory conventional treatment methods. Key components field include stem cells, bioactive growth factors, biocompatible scaffolds, with a strong focus on advancements substitute materials. Both natural synthetic substitutes present distinct characteristics applications. Natural grafts-comprising autologous, allogeneic, xenogeneic materials-offer biological advantages, while alternatives, including biodegradable non-biodegradable biomaterials, provide structural versatility reduced immunogenicity. This review provides comprehensive analysis diverse grafting alternatives utilized surgery, emphasizing persistent challenges. By exploring both substitutes, work offers in-depth examination cutting-edge solutions, fostering further innovation complex defects.

Язык: Английский

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A new dawn: Vitalising translational oncology research in Africa with the help of advanced cell culture models

Translational Oncology, Год журнала: 2025, Номер 56, С. 102391 - 102391

Опубликована: Апрель 14, 2025

Язык: Английский

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Isogenic induced pluripotent stem cell line ICGi036-A-1 from a patient with familial hypercholesterolaemia, derived by correcting a pathogenic variant of the gene LDLR c.530C>T

Vavilov Journal of Genetics and Breeding, Год журнала: 2025, Номер 29(2), С. 189 - 199

Опубликована: Апрель 10, 2025

Familial hypercholesterolaemia is a common monogenic disorder characterized by high plasma cholesterol levels leading to chronic cardiovascular disease with risk and often early manifestation due atherosclerotic lesions of the blood vessels. The in familial are mainly caused pathogenic variants low-density lipoprotein receptor ( LDLR ) gene, which plays an important role metabolism. Normally, cholesterol-laden lipoproteins bind on surface liver cells be removed from bloodstream internalisation hepatocytes. In hypercholesterolaemia, function impaired uptake significantly reduced. As result, accumulates subendothelial space inner wall vessels, triggering atherogenesis, formation plaques. At present, there no effective universal approaches diagnosis treatment hypercholesterolaemia. A relevant approach study molecular genetic mechanisms obtain systems for screening chemical compounds as potential drugs generation cellular models based patient-specific induced pluripotent stem cells. aim our work was derive isogenic genetically modified cell line correcting allelic variant c.530C gene original iPSC previously obtained compound heterozygote patient resulting differs only one corrected nucleotide substitution, allowing us direct effect this physiological changes differentiated CRISPR/Cas-mediated base editing used correct single substitution. has pluripotency traits, normal karyotype, set short tandem repeats identical that can derivatives necessary elaboration models.

Язык: Английский

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Development of regenerative therapies targeting fibrotic endometrium in intrauterine adhesion or thin endometrium to restore uterine function

Science China Life Sciences, Год журнала: 2025, Номер unknown

Опубликована: Апрель 11, 2025

Язык: Английский

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