Single circulating tumor cell sequencing based on improved high-porosity membranes and nanoporous microchambers

Biosensors and Bioelectronics, Год журнала: 2025, Номер 276, С. 117263 - 117263

Опубликована: Фев. 14, 2025

Язык: Английский

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Polymerase Chain Reaction Chips for Biomarker Discovery and Validation in Drug Development

Micromachines, Год журнала: 2025, Номер 16(3), С. 243 - 243

Опубликована: Фев. 20, 2025

Polymerase chain reaction (PCR) chips are advanced, microfluidic platforms that have revolutionized biomarker discovery and validation because of their high sensitivity, specificity, throughput levels. These miniaturize traditional PCR processes for the speed precision nucleic acid detection relevant to advancing drug development. Biomarkers, which useful in helping explain disease mechanisms, patient stratification, therapeutic monitoring, hard identify validate due complexity biological systems limitations techniques. The challenges respond include high-throughput capabilities coupled with real-time quantitative analysis, enabling researchers novel biomarkers greater accuracy reproducibility. More recent design improvements further expanded functionality also digital multiplex technologies. Digital ideal quantifying rare biomarkers, is essential oncology infectious research. In contrast, enable simultaneous analysis multiple targets, therefore simplifying validation. Furthermore, single-cell made it possible detect at unprecedented resolution, hence revealing heterogeneity within cell populations. transforming development, target identification, efficacy assessment. They play a major role development companion diagnostics and, therefore, pave way personalized medicine, ensuring right receives treatment. While this tremendously promising technology has exhibited many regarding its scalability, integration other omics technologies, conformity regulatory requirements, still prevail. Future breakthroughs chip manufacturing, artificial intelligence, multi-omics applications will expand capabilities. not only be important acceleration but raising bar improving outcomes hence, global health care as these technologies continue mature.

Язык: Английский

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Pharmacologic Ascorbate Resistant Pancreatic Cancer Demonstrates Enhanced Metastatic Potential

Опубликована: Янв. 1, 2025

Язык: Английский

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The Role of Inflammation in Cancer: Mechanisms of Tumor Initiation, Progression, and Metastasis

Cells, Год журнала: 2025, Номер 14(7), С. 488 - 488

Опубликована: Март 25, 2025

Inflammation is an essential component of the immune response that protects host against pathogens and facilitates tissue repair. Chronic inflammation a critical factor in cancer development progression. It affects every stage tumor development, from initiation promotion to invasion metastasis. Tumors often create inflammatory microenvironment induces angiogenesis, suppression, malignant growth. Immune cells within interact actively with cells, which drives progression through complex molecular mechanisms. triggered by factors such as infections, obesity, environmental toxins strongly linked increased risk. However, acute responses can sometimes boost antitumor immunity; thus, presents both challenges opportunities for therapeutic intervention. This review examines how contributes biology, emphasizing its dual role tumorigenesis potential target.

Язык: Английский

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Gene Expression Profiling and Phenotypic Characterization of Circulating Tumor Cells Derived from a Murine Osteosarcoma Model

Cancers, Год журнала: 2025, Номер 17(7), С. 1210 - 1210

Опубликована: Апрель 2, 2025

Osteosarcoma is an aggressive bone malignancy with high metastatic potential to the lungs. CTCs, as seeds of metastasis, play important role in spread this cancer, and, therefore, their isolation, culture, and gene expression analysis promises valuable insights into progression cascade osteosarcoma. The aim study was isolate culture CTCs from osteosarcoma-bearing mice compare migration, radioresistance, parental cell line. LM8-inoculated were isolated cultured. CTC-derived lines then compared Furthermore, a Transwell assay, clonogenic assay after irradiation, immunohistochemical stainings used differed significantly 361 differentially expressed genes identified, among which GO KEGG revealed major differences related antigen processing presentation extracellular matrix constituents. In addition, observed have higher migratory capacity comparable radioresistance CD44 found be conserved lines. This provides comparison between terms expression, radioresistance. Our findings allow for further research field osteosarcoma generation. identified DEGs line can serve reference point targeted therapies against CTCs.

Язык: Английский

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Precision blood biopsy for lung cancer diagnosis using a targeting nanoprobe to detect multiple mRNA biomarkers in circulating malignant cells

Applied Physics Reviews, Год журнала: 2025, Номер 12(2)

Опубликована: Апрель 1, 2025

Compared to tissue biopsy, blood biopsy offers significant advantages in terms of safety and convenience. However, achieving accurate for cancer pathological diagnosis presents substantial challenges. Herein, we have developed a precision technology utilizing malignant cell-targeted nanoprobe lung diagnosis. The functionalized with SYL3C-conjugated hyaluronic acid the KALA-GE11 peptide can efficiently deliver loaded molecular beacons into heterogeneous circulating cells (CMCs) whole detect diverse intracellular mRNA markers, including proliferation marker Ki67, oncogenic c-Myc, thyroid transcription factor 1 (TTF-1) typically expressed specific types cancers. simultaneous detection various markers not only reduces false-negative rates caused by high heterogeneity CMCs but also provides insight individual patients. differentiate adenocarcinoma (LAD) small cell (SCLC) from squamous carcinoma (SCC). TTF-1 is abundant LAD SCLC relatively less common SCC. c-Myc more frequently overexpressed SCLC. Notably, Ki67 upregulated most across all types. Furthermore, this approach sensitively identify early stage lesions clinically classified as noninvasive situ. This technique holds clinical potentials precise characterization cancer. Importantly, strategy adaptable detecting other biomarkers, thereby extending its applicability

Язык: Английский

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Stem Cells in Cancer: From Mechanisms to Therapeutic Strategies

Cells, Год журнала: 2025, Номер 14(7), С. 538 - 538

Опубликована: Апрель 3, 2025

Stem cells have emerged as a pivotal area of research in the field oncology, offering new insights into mechanisms cancer initiation, progression, and resistance to therapy. This review provides comprehensive overview role stem cancer, focusing on (CSCs), their characteristics, implications for We discuss origin identification CSCs, tumorigenesis, metastasis, drug resistance, potential therapeutic strategies targeting CSCs. Additionally, we explore use normal therapy, tissue regeneration delivery vehicles anticancer agents. Finally, highlight challenges future directions cell cancer.

Язык: Английский

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Positive sentinel lymph node biopsy on promotion of metastasis: An unexpected effect?

Medical Hypotheses, Год журнала: 2025, Номер unknown, С. 111624 - 111624

Опубликована: Апрель 1, 2025

Язык: Английский

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Efficient and Discriminative Isolation of Circulating Cancer Stem Cells and Non-Stem-like Circulating Tumor Cells Using a Click-Handle-Loaded M13 Phage-Based Surface

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

Circulating tumor cells (CTCs) are crucial for cancer research and clinical applications, with circulating stem (cCSCs) being a rare but key subpopulation responsible metastasis, recurrence, therapy resistance. Current limitations in efficiently isolating these cells, particularly distinguishing cCSCs from non-stem-like CTCs (nsCTCs), hinder our understanding of progression precision medicine strategies. Herein, we developed novel CTC isolation approach that integrates cell metabolic chemical tagging click-handle-loaded M13 phage-based surface (CHPhace). The multivalent nature flexible nanofibers, featuring thousands modification sites click reactions, significantly enhances capture across diverse types. Leveraging the unique slow-cycling characteristic cCSCs, CHPhace demonstrated selective through labeling demetabolism processes. robust performance allows efficient both nsCTCs complex blood sample matrices, achieving efficiencies exceeding 80%. This represents promising tool advancing enhancing diagnosis prognosis.

Язык: Английский

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Circulating tumor cell markers for early detection and drug resistance assessment through liquid biopsy

Frontiers in Oncology, Год журнала: 2025, Номер 15

Опубликована: Апрель 7, 2025

Circulating tumor cells (CTCs) are cancerous that extravasate from the primary or metastatic foci and travel through bloodstream to distant organs. CTCs provide crucial insights into cancer metastasis, evolution of genotypes during treatment, development chemo- and/or radio-resistance disease progression. The process Epithelial-to-mesenchymal transition (EMT) plays a key role in formation, as this enhances cell’s migration properties is often associated with increased invasiveness thereby leading chemotherapy resistance. During EMT process, lose epithelial markers like EpCAM acquire mesenchymal such vimentin driven by transcription factors Snail Twist. typically identified using specific cell surface markers, which vary depending on type. Common include EpCAM, used for cancers; CD44 CD24, stem cells; cytokeratins, CK8 CK18. Other HER2/neu can also be target types stages. Commonly, immune-based isolation techniques being implemented enrichment CTCs. This review emphasizes clinical relevance CTCs, particularly understanding drug resistance mechanisms, underscores importance EMT-derived multidrug (MDR). Moreover, discusses CTCs-specific their enrichment. Ultimately, EMT-specific found could significant information halt progression enable personalized therapies.

Язык: Английский

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