Toxic effects of nanoplastics on biological nitrogen removal in constructed wetlands: Evidence from iron utilization and metabolism

Water Research, Год журнала: 2024, Номер 256, С. 121577 - 121577

Опубликована: Апрель 6, 2024

Язык: Английский

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New Insights into the Organic Complexation of Bioactive Trace Metals in the Global Ocean from the GEOTRACES Era

Oceanography, Год журнала: 2024, Номер 37(2)

Опубликована: Янв. 1, 2024

The GEOTRACES program has greatly expanded measurements of dissolved trace metal concentrations across ocean basins, but to understand the behavior and cycling metals their impacts on primary productivity, we must chemical forms in which they are present environment. Organic ligands play a central role speciation marine environment, controlling reactivity bioavailability. Here, an overview contributions made understanding through advancing our knowledge distribution, sources, sinks metal-binding organic global ocean, particularly for iron. Detailed assessments intercalibration methods most commonly applied have allowed integration ligand datasets. Work characterize specific groups within wider pool matter, along with sources sinks, is starting unravel biogeochemical cycles. Recent advances complementary analytical techniques using liquid chromatography mass spectrometry molecular picture bioavailability—and also pose new questions. Moving forward, need address gaps how complexation relates bioavailability order recognize distributions productivity carbon cycle.

Язык: Английский

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Long-Read Sequencing Improves Recovery of Picoeukaryotic Genomes and Zooplankton Marker Genes from Marine Metagenomes

mSystems, Год журнала: 2022, Номер 7(6)

Опубликована: Ноя. 30, 2022

Long-read sequencing offers the potential to improve metagenome assemblies and provide more robust assessments of microbial community composition function than short-read sequencing. We applied Pacific Biosciences (PacBio) CCS (circular consensus sequencing) HiFi shotgun 14 marine water column samples compared results with those for metagenomes from corresponding environmental DNA samples. found that long-read varied widely in quality biological information. The compositions long- were frequently dissimilar, suggesting higher stochasticity and/or bias associated PacBio Long reads provided few improvements assembly qualities, gene annotations, prokaryotic metagenome-assembled genome (MAG) binning results. However, only long produced high-quality eukaryotic MAGs contigs containing complete zooplankton marker sequences. These suggest can analyses important insight into phyto- genetics, but benefits may be outweighed by inconsistent data quality.

Язык: Английский

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Contribution of cryoconite holes in the supraglacial discharge of bioavailable iron in Larsemann Hills, East Antarctica

Polar Science, Год журнала: 2024, Номер 40, С. 101052 - 101052

Опубликована: Фев. 1, 2024

Язык: Английский

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Nasal commensals reduce Staphylococcus aureus proliferation by restricting siderophore availability

The ISME Journal, Год журнала: 2024, Номер 18(1)

Опубликована: Янв. 1, 2024

Abstract The human microbiome is critically associated with health and disease. One aspect of this that antibiotic-resistant opportunistic bacterial pathogens, such as methicillin-resistant Staphylococcus aureus, can reside within the nasal microbiota, which increases risk infection. Epidemiological studies have revealed positive negative correlations between non-pathogenic species S. but underlying molecular mechanisms remain poorly understood. cavity iron-limited, bacteria are known to produce iron-scavenging siderophores proliferate in environments. Siderophores public goods be consumed by all members a community. Accordingly, mediate competition collaboration, their role unknown. Here, we show siderophore acquisition crucial for aureus colonization vivo. We screened 94 strains from seven genera capacity well consume produced aureus. found 80% engaged siderophore-mediated interactions Non-pathogenic corynebacterial were prominent consumers siderophores. In co-culture experiments, consumption competitors reduced growth an iron-dependent fashion. Our data wide network provide mechanistic evidence inter-species collaboration impacting pathogen proliferation. This opens avenues designing probiotics displace humans.

Язык: Английский

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Global niche partitioning of purine and pyrimidine cross-feeding among ocean microbes

Science Advances, Год журнала: 2025, Номер 11(1)

Опубликована: Янв. 3, 2025

Cross-feeding involves microbes consuming exudates of other surrounding microbes, mediating elemental cycling. Characterizing the diversity cross-feeding pathways in ocean illuminates evolutionary forces driving self-organization ecosystems. Here, we uncover a purine and pyrimidine network globally abundant groups. The cyanobacterium Prochlorococcus exudes both compound classes, which metabolic reconstructions suggest follows synchronous daily genome replication. Co-occurring heterotrophs differentiate into purine- pyrimidine-using generalists or specialists that use compounds for different purposes. most heterotroph, SAR11, is specialist uses purines as sources energy, carbon, and/or nitrogen, with subgroups differentiating along ocean-scale gradients supply energy turn producing putative cryptic nitrogen cycles link many microbes. Last, an SAR11 subgroup dominates where abundant, adenine additions to cultures inhibit DNA synthesis, poising cells We argue this inferred pulses from synchronize photosynthate phytoplankton.

Язык: Английский

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Marine siderophores and its implications in changing polar ecosystem: a review

Polar Biology, Год журнала: 2025, Номер 48(1)

Опубликована: Фев. 10, 2025

Язык: Английский

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Unusual Ecofunctional Traits ofEndozoicomonas: A Pan-Genomic Perspective

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

Abstract Background Endozoicomonas is a widely distributed genus of marine bacteria, associated with various organisms, and recognized for its ecological importance in host health, nutrient cycling, disease dynamics. Despite significance, genomic features remain poorly characterized due to limited availability high-quality genome assemblies. Results In this study, we sequenced 5 novel strains re-sequenced 1 known strain improve resolution. By integrating these 6 genomes 31 others that were publicly available, identified distinct, coral-associated clade not by the previous two-clade classification. Pan-genomic analysis revealed significant variation genetic trait distribution among clades. Notably, lacks quorum sensing capabilities, suggesting resistance quenching mechanisms. It also ability synthesize transport vitamin B12, indicating it primary source holobionts. A remarkable feature abundance giant proteins, ranging from 15 65 kbp. We 92 such which clustered into three major groups based on amino acid similarity, each specialized functions, as antimicrobial synthesis, exotoxin production, cell adhesion. Additionally, explored prophages CRISPR-Cas systems. found acquired diverse sources via infection or other types gene transfer. sequences suggest independent evolutionary trajectories both prophage acquisition phylogenetic lineage, implying potential influence geographic environmental pressures. Conclusions This study provides new insights diversity adaptation hosts. Identification features, including deficiencies B12 synthesis sensing, presence prophages, systems, underscores roles These findings open avenues research interactions.

Язык: Английский

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Simultaneous acclimation to nitrogen and iron scarcity in open ocean cyanobacteria revealed by sparse tensor decomposition of metatranscriptomes

Science Advances, Год журнала: 2025, Номер 11(14)

Опубликована: Апрель 4, 2025

Microbes respond to changes in their environment by adapting physiology through coordinated adjustments the expression levels of functionally related genes. To detect these shifts situ, we developed a sparse tensor decomposition method that derives gene co-expression patterns from inherently complex whole community RNA sequencing data. Application metatranscriptomes abundant marine cyanobacteria Prochlorococcus and Synechococcus identified responses scarcity two essential nutrients, nitrogen iron, including increased transporter expression, restructured photosynthesis carbon metabolism, mitigation oxidative stress. Further, profiles clusters suggest both populations experience simultaneous iron stresses transition zone between North Pacific oceanic gyres. The results demonstrate power our approach infer organism environmental pressures, hypothesize functions uncharacterized genes, extrapolate ramifications for biogeochemical cycles changing ecosystem.

Язык: Английский

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Metal Organic Complexation in Seawater: Historical Background and Future Directions

Annual Review of Marine Science, Год журнала: 2023, Номер 16(1), С. 577 - 599

Опубликована: Сен. 19, 2023

The speciation of most biologically active trace metals in seawater is dominated by complexation organic ligands. This review traces the history work this area, from early observations that showed surprisingly poor recoveries using metal preconcentration protocols to present day, where advances mass spectroscopy and stable isotope geochemistry are providing new insights into structure, origin, fate, biogeochemical impact Many long-standing hypotheses about specific biological origin ligands such as siderophores finally being validated. has revealed complexity complexation, with multiple and, some cases, timescales ligand exchange much slower than originally thought. influence on scavenging now a key parameter models essential metals, especially iron. New sources sinks required enhance usefulness these models.

Язык: Английский

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