Agriculture,
Год журнала:
2024,
Номер
14(10), С. 1663 - 1663
Опубликована: Сен. 24, 2024
The
objective
of
this
study
was
to
examine
the
effect
humic
acid
(HA)
supplementation
on
rumen
fermentation
and
protozoal
community
in
rumen.
For
purpose,
four
ruminally
cannulated
Simmental
cows
were
randomly
assigned
a
replicated
4
×
Latin
square
design
experiment
HA
dietary
feed
utilization,
fermentation,
for
84
days.
basal
diet
(BD)
composed
meadow
hay
(68.2%
dry
matter
[DM]),
maize
silage
(17%
DM),
granulated
mixture
(14.9%
DM).
There
treatments,
including
BD
without
additives
(control
diet,
H0),
supplemented
with
50
g
HA/cow/day
(H50
treatment),
100
(H100
200
(H200
treatment).
did
not
affect
total
or
individual
volatile
fatty
concentrations,
protozoa,
ruminal
pH.
However,
at
dosage
g/cow/day
increased
NH3-N
concentration
fecal
nitrogen
compared
control
(p
<
0.001).
also
significantly
impacted
abundance
genera
results
suggest
that
has
potential
ruminants
as
natural
additive
may
play
role
metabolism
stabilizing
adverse
effects
fermentation.
Animal Bioscience,
Год журнала:
2023,
Номер
37(2), С. 370 - 384
Опубликована: Дек. 29, 2023
Rumen
microbiota
play
a
central
role
in
the
digestive
process
of
ruminants.
Their
remarkable
ability
to
break
down
complex
plant
fibers
and
proteins,
converting
them
into
essential
organic
compounds
that
provide
animals
with
energy
nutrition.
Research
on
rumen
not
only
contributes
improving
animal
production
performance
enhancing
feed
utilization
efficiency
but
also
holds
potential
reduce
methane
emissions
environmental
impact.
Nevertheless,
studies
face
numerous
challenges,
including
complexity,
difficulties
cultivation,
obstacles
functional
analysis.
This
review
provides
an
overview
microbial
species
involved
degradation
macromolecules,
fermentation
processes,
rumen,
all
based
cultivation
methods.
Additionally,
introduces
applications,
advantages,
limitations
emerging
omics
technologies
such
as
metagenomics,
metatranscriptomics,
metaproteomics,
metabolomics,
investigating
functionality
microbiota.
Finally,
article
offers
forward-looking
perspective
new
horizons
field
research.
These
technologies,
continuous
refinement
mutual
complementation,
have
deepened
our
understanding
functionality,
thereby
enabling
effective
manipulation
community.
ABSTRACT
In
ruminants,
the
rumen
is
a
specialized
stomach
that
adapted
to
breakdown
of
plant-derived
complex
polysaccharides
through
coordinated
activities
diverse
microbial
community.
Bacteroidota
major
phylum
in
this
bovine
microbiota.
They
contain
several
clusters
genes
called
polysaccharide
utilization
loci
(PULs)
encode
proteins
working
concert
capture,
degrade,
and
transport
polysaccharides.
Despite
critical
role
SusD-like
for
efficient
substrate
transport,
they
remain
largely
unexplored.
Here,
we
present
biochemical
characterization
protein
encoded
by
β-glucan
locus
from
an
Escherichia
coli
metagenomic
clone
previously
isolated
functional
screening
microbiome.
study,
show
41O1
can
grow
on
laminaritriose,
cellotriose,
mixture
cellobiosyl-cellobiose
glucosyl-cellotriose
as
sole
carbon
sources.
Based
this,
used
various
vitro
analyses
investigate
binding
ability
41O1_SusD-like
towards
these
oligosaccharides
corresponding
We
observed
clear
affinity
β-1,6
branched
β-1,3-glucans
(laminarins,
yeast
β-glucan)
laminaritriose.
Comparison
AlphaFold2
model
with
its
closest
structural
homologs
highlights
similar
pattern
recognition.
particular,
three
tryptophan
residues
are
shown
be
crucial
laminarin
context
cow
rumen,
discuss
possible
substrates
targeted
41O1_PUL,
such
(1,3;1,4)-β-
d
-glucans
cereal
grains
or
β-1,3-
(1,3;1,6)-β-
components
cell
wall
ruminal
yeasts.
IMPORTANCE
The
microbiota
majorly
impact
overall
animal
health,
feed
efficiency,
release
harmful
substances
into
environment.
This
involved
fermentation
organic
matter
provide
host
valuable
assimilable
nutrients.
efficiently
captures,
breaks
down,
imports
concerted
action
(PULs).
Within
system,
has
proven
necessary
active
internalization
substrate.
Nevertheless,
vast
majority
characterized
date
originate
cultured
bacteria.
With
regard
diversity
importance
uncultured
bacteria
further
studies
required
better
understand
degradation.
Our
detailed
therefore
contributes
understanding
carbohydrate
metabolism
Bacteroides
rumen.
Agriculture,
Год журнала:
2024,
Номер
14(10), С. 1663 - 1663
Опубликована: Сен. 24, 2024
The
objective
of
this
study
was
to
examine
the
effect
humic
acid
(HA)
supplementation
on
rumen
fermentation
and
protozoal
community
in
rumen.
For
purpose,
four
ruminally
cannulated
Simmental
cows
were
randomly
assigned
a
replicated
4
×
Latin
square
design
experiment
HA
dietary
feed
utilization,
fermentation,
for
84
days.
basal
diet
(BD)
composed
meadow
hay
(68.2%
dry
matter
[DM]),
maize
silage
(17%
DM),
granulated
mixture
(14.9%
DM).
There
treatments,
including
BD
without
additives
(control
diet,
H0),
supplemented
with
50
g
HA/cow/day
(H50
treatment),
100
(H100
200
(H200
treatment).
did
not
affect
total
or
individual
volatile
fatty
concentrations,
protozoa,
ruminal
pH.
However,
at
dosage
g/cow/day
increased
NH3-N
concentration
fecal
nitrogen
compared
control
(p
<
0.001).
also
significantly
impacted
abundance
genera
results
suggest
that
has
potential
ruminants
as
natural
additive
may
play
role
metabolism
stabilizing
adverse
effects
fermentation.
