Algal exudates promote conjugation in marine Roseobacters DOI Creative Commons

Yemima Duchin Rapp,

Valeria Lipsman,

Lilach Yuda

и другие.

mBio, Год журнала: 2024, Номер 15(10)

Опубликована: Авг. 27, 2024

Horizontal gene transfer (HGT) is a pivotal mechanism driving bacterial evolution, conferring adaptability within dynamic marine ecosystems. Among HGT mechanisms, conjugation mediated by type IV secretion systems (T4SSs) plays central role in the ecological success of bacteria. However, conditions promoting events environment are not well-understood. Roseobacters, abundant bacteria commonly associated with algae, possess multitude T4SSs. Many Roseobacters heterotrophic that rely on algal secreted compounds to support their growth. These attract bacteria, facilitating colonization and attachment cells. Algae metabolites bring into close proximity, potentially HGT. Investigation across various revealed exudates indeed enhance plasmid through conjugation. While do influence transcription conjugative machinery genes, they promote attachment, stabilizing proximity Notably, under where less advantageous, impact reduced. findings suggest algae primarily fostering highlight importance studying context algal-bacterial interactions.

Язык: Английский

Evidence of nitrate-based nighttime atmospheric nucleation driven by marine microorganisms in the South Pacific DOI Creative Commons

Guillaume Chamba,

Matti Rissanen, Theresa Barthelmeß

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2023, Номер 120(48)

Опубликована: Ноя. 22, 2023

Our understanding of ocean–cloud interactions and their effect on climate lacks insight into a key pathway: do biogenic marine emissions form new particles in the open ocean atmosphere? Using measurements collected ship-borne air–sea interface tanks deployed Southwestern Pacific Ocean, we identified particle formation (NPF) during nighttime that was related to plankton community composition. We show nitrate ions are only species for which abundance could support NPF rates our semicontrolled experiments. Nitrate also prevailed natural pristine atmosphere were elevated under higher sub-10 nm concentrations. hypothesize these nucleation events fueled by complex, short-term biogeochemical cycling involving microbial loop. These findings suggest perspective with previously unidentified role origin aerosol nucleation.

Язык: Английский

Процитировано

2
The bacterial Bmt methionine synthase is involved in lag phase shortening DOI Creative Commons
Delia A. Narváez-Barragán, Martin Sperfeld, Einat Segev

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 21, 2024

Abstract Bacteria can shorten their lag phase by utilizing methyl groups from compounds such as dimethylsulfoniopropionate (DMSP). These are then incorporated into cellular building blocks via the methionine cycle. However, specific contribution of bacterial synthesis, which is critical for assimilating and incorporating groups, remains unclear. In this study, we employed transcriptomics, genetic manipulation biochemical assays to explore involvement synthesis in shortening using model marine bacterium Phaeobacter inhibens . We mapped expression profiles MetH-like synthase components—an enzyme complex that encoded three genes—in response DMSP during phase. Our findings revealed transcriptional decoupling genes. The deletion homocysteine-binding component complex, namely bmt , disrupted DMSP. Through heterologous gene product, show individual Bmt produces directly demethylating betaine vitro reveal a metabolic route was not previously described bacteria. Since does require tetrahydrofolate or cobalamin co-factors its potential act alone demethylase represents cost-effective shortcut group assimilation, could be specifically beneficial under limiting conditions. Indeed, stress conditions, allows cells This study enhances our understanding enzymatic mechanisms underlying shortening, revealing microbial adaptation strategies environmental

Язык: Английский

Процитировано

0
Microalgal—bacterial interactions: Research trend and updated review DOI Creative Commons
Muhammad Iqhrammullah, Williams Chiari, Syihaabul Hudaa

и другие.

Heliyon, Год журнала: 2024, Номер 10(15), С. e35324 - e35324

Опубликована: Июль 27, 2024

Microalgae are being recognized as the key contributor to sustainability in many sectors, starting from energy up food industries. The microorganism has also been utilized environmental remediator, capable of converting organic compounds into economically valuable biomass. To optimize use microalgae these researchers have explored various approaches, which is bacteria. interaction between bacteria and can potentially be harnessed, but its complexity requires extensive research. Herein, we present bibliometric analysis on microalgal-bacterial interactions. metadata published literature was collected through Scopus database August 4, 2023. downloaded.csv file uploaded VOSViewer biblioshiny for network visualization. We found that research gained a lot attention since 2012 with an exponential increase publication number. United States China leading strong collaboration. Based sub-topic clusters, mostly studied wastewater treatment, biomass production, algal bloom control. Updated reviews this topic reveal now focus optimizing efficacy microalgae-bacteria system, investigating modes actions, identifying challenges real-world implementation. promising approach utilization

Язык: Английский

Процитировано

0
Dissolved nitric oxide in the lower Elbe Estuary and the Port of Hamburg area DOI Creative Commons
Riel Carlo O. Ingeniero, Gesa Schulz, Hermann W. Bange

и другие.

Biogeosciences, Год журнала: 2024, Номер 21(14), С. 3425 - 3440

Опубликована: Июль 30, 2024

Abstract. Nitric oxide (NO) is an intermediate of various microbial nitrogen cycle processes, and the open-ocean coastal areas are generally a source NO to atmosphere. However, our knowledge about its distribution main production processes in estuaries rudimentary at best. To this end, dissolved concentrations were measured for first time surface waters along lower Elbe Estuary Port Hamburg area July 2021. The discrete water samples analyzed using chemiluminescence analyzer connected stripping unit. ranged from below limit detection (9.1 pM) 17.7 pM, averaging 12.5 supersaturated layer both area, indicating that study site was atmosphere during period. On basis comprehensive comparison with parallel nutrient, oxygen, nitrous concentration measurements, we conclude observed most likely resulted nitrification. In Hamburg, however, nitrifier denitrification and/or might also affect distribution.

Язык: Английский

Процитировано

0
Algal exudates promote conjugation in marine Roseobacters DOI Creative Commons

Yemima Duchin Rapp,

Valeria Lipsman,

Lilach Yuda

и другие.

mBio, Год журнала: 2024, Номер 15(10)

Опубликована: Авг. 27, 2024

Horizontal gene transfer (HGT) is a pivotal mechanism driving bacterial evolution, conferring adaptability within dynamic marine ecosystems. Among HGT mechanisms, conjugation mediated by type IV secretion systems (T4SSs) plays central role in the ecological success of bacteria. However, conditions promoting events environment are not well-understood. Roseobacters, abundant bacteria commonly associated with algae, possess multitude T4SSs. Many Roseobacters heterotrophic that rely on algal secreted compounds to support their growth. These attract bacteria, facilitating colonization and attachment cells. Algae metabolites bring into close proximity, potentially HGT. Investigation across various revealed exudates indeed enhance plasmid through conjugation. While do influence transcription conjugative machinery genes, they promote attachment, stabilizing proximity Notably, under where less advantageous, impact reduced. findings suggest algae primarily fostering highlight importance studying context algal-bacterial interactions.

Язык: Английский

Процитировано

0