Osteoblast Differentiation and Signaling: Established Concepts and Emerging Topics

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(13), С. 6651 - 6651

Опубликована: Июнь 22, 2021

Osteoblasts, the cells that build up our skeleton, are remarkably versatile and important need tight regulation in all phases of their differentiation to guarantee proper skeletal development homeostasis. Although we know many key pathways involved osteoblast signaling, it is becoming clearer this just tip iceberg, constantly discovering novel concepts physiology. In review, discuss well-established osteoblastic differentiation, i.e., classical ones committing mesenchymal stromal osteoblast, then osteocytes as well recently emerged players. particular, micro (mi)RNAs, long non-coding (lnc)RNAs, circular (circ)RNAs, extracellular vesicles, focusing on mechanisms through which osteoblasts regulated by these factors, conversely, how they use vesicles communicate with surrounding microenvironment.

Язык: Английский

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Aged bone matrix-derived extracellular vesicles as a messenger for calcification paradox

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Март 18, 2022

Adipocyte differentiation of bone marrow mesenchymal stem/stromal cells (BMSCs) instead osteoblast formation contributes to age- and menopause-related adiposity osteoporosis. Vascular calcification often occurs with osteoporosis, a contradictory association called "calcification paradox". Here we show that extracellular vesicles derived from aged matrix (AB-EVs) during resorption favor BMSC adipogenesis rather than osteogenesis augment vascular smooth muscle cells. Intravenous or intramedullary injection AB-EVs promotes bone-fat imbalance exacerbates Vitamin D3 (VD3)-induced in young old mice. Alendronate (ALE), inhibitor, down-regulates release attenuates aging- ovariectomy-induced imbalance. In the VD3-treated mice, ALE suppresses aggravation calcification. MiR-483-5p miR-2861 are enriched essential for AB-EVs-induced exacerbation Our study uncovers role as messenger paradox by transferring miR-483-5p miR-2861.

Язык: Английский

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Adipogenesis, Osteogenesis, and Chondrogenesis of Human Mesenchymal Stem/Stromal Cells: A Comparative Transcriptome Approach

Frontiers in Cell and Developmental Biology, Год журнала: 2020, Номер 8

Опубликована: Июль 8, 2020

Adipogenesis, osteogenesis and chondrogenesis of human mesenchymal stem/stromal cells (MSC) are complex highly regulated processes. Over the years, several studies have focused on understanding mechanisms involved in MSC commitment to osteogenic, adipogenic and/or chondrogenic phenotypes. High-throughput methodologies been used investigate gene expression profile during differentiation. Association data analysis mRNAs, microRNAs, circular RNAs long non-coding RNAs, obtained at different time points over these processes, important depict complexity This review will discuss results that were highlighted transcriptome analyses undergoing adipogenic, osteogenic The focus is shed light key molecules, main signaling pathways biological processes related adipogenesis, chondrogenesis.

Язык: Английский

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Bioactive glass nanoparticles inhibit osteoclast differentiation and osteoporotic bone loss by activating lncRNA NRON expression in the extracellular vesicles derived from bone marrow mesenchymal stem cells

Biomaterials, Год журнала: 2022, Номер 283, С. 121438 - 121438

Опубликована: Фев. 24, 2022

Язык: Английский

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The Role of Micro RNA and Long-Non-Coding RNA in Osteoporosis

International Journal of Molecular Sciences, Год журнала: 2020, Номер 21(14), С. 4886 - 4886

Опубликована: Июль 10, 2020

Osteoporosis is a major concern worldwide and can be attributed to an imbalance between osteoblastic bone formation osteoclastic resorption due the natural aging process. Heritable factors account for 60-80% of optimal mineralization; however, finer details pathogenesis remain elucidated. Micro RNA (miRNA) long-non-coding (lncRNA) are two targets that have recently come into spotlight their ability control gene expression at post-transcriptional level provide epigenetic modification. miRNAs class non-coding RNAs approximately 18-25 nucleotides long. It thought up 60% human protein-coding genes may regulated by miRNAs. They been found regulate controls osteoblast-dependent osteoclast-related remodeling. lncRNAs highly structured transcripts longer than 200 do not translate proteins. very complex secondary tertiary structures same degradation processes as messenger RNAs. The fact they rapid turnover sponge function in binding lead lncRNA itself. act signaling, decoy, framework molecules, or primers. Current evidence suggests chromatin transcriptional well regulators. With regards osteoporosis, involved proliferation, apoptosis, inflammatory response bone. This review, which based on systematic appraisal current literature, provides molecular genetic opinions roles osteoporosis. Further research modification regulatory these molecules will bring us closer potential disease-modifying treatment However, more issues regarding detailed actions osteoporosis unknown controversial warrant future investigation.

Язык: Английский

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Bioinspired mineral hydrogels as nanocomposite scaffolds for the promotion of osteogenic marker expression and the induction of bone regeneration in osteoporosis

Acta Biomaterialia, Год журнала: 2020, Номер 113, С. 614 - 626

Опубликована: Июнь 19, 2020

Язык: Английский

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A functional motif of long noncoding RNA Nron against osteoporosis

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Июнь 3, 2021

Abstract Long noncoding RNAs are widely implicated in diverse disease processes. Nonetheless, their regulatory roles bone resorption undefined. Here, we identify lncRNA Nron as a critical suppressor of resorption. We demonstrate that osteoclastic knockout mice exhibit an osteopenia phenotype with elevated activity. Conversely, transgenic lower and higher mass. Furthermore, the pharmacological overexpression inhibits resorption, while caused apparent side effects mice. To minimize effects, further functional motif Nron. The delivery to osteoclasts effectively reverses loss without obvious effects. Mechanistically, interacts E3 ubiquitin ligase CUL4B regulate ERα stability. These results indicate is key suppressor, could potentially be utilized treat diseases less risk

Язык: Английский

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RANKL-responsive epigenetic mechanism reprograms macrophages into bone-resorbing osteoclasts

Cellular and Molecular Immunology, Год журнала: 2022, Номер 20(1), С. 94 - 109

Опубликована: Дек. 14, 2022

Язык: Английский

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Advances in the research of the role of macrophage/microglia polarization-mediated inflammatory response in spinal cord injury

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Дек. 1, 2022

It is often difficult to regain neurological function following spinal cord injury (SCI). Neuroinflammation thought be responsible for this failure. Regulating the inflammatory response post-SCI may contribute recovery of function. Over past few decades, studies have found that macrophages/microglia are one primary effector cells in SCI. Growing evidence has documented plastic can polarize microenvironmental signals into M1 and M2 macrophages/microglia. produces pro-inflammatory cytokines induce inflammation worsen tissue damage, while anti-inflammatory activities wound healing regeneration. Recent indicated transition from phenotype macrophage/microglia supports regression repair. Here, we will review role SCI In addition, discuss potential molecular mechanisms polarization, with emphasis on neuroprotective therapies modulate which provide new insights therapeutic strategies

Язык: Английский

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Bone marrow mesenchymal stem cells derived exosomal Lnc TUG1 promotes bone fracture recovery via miR-22-5p/Anxa8 axis

Human Cell, Год журнала: 2023, Номер 36(3), С. 1041 - 1053

Опубликована: Март 23, 2023

Bone fracture healing is a complex physiologic process that involves changes in the expression of several thousand genes. Long noncoding RNAs (lncRNAs) may have critical biological roles this process. The objectives present study were to determine whether BMSC-derived exosomal lncTUG1 can enhance osteogenic differentiation and thereby promoting bone recovery investigate its potential mechanisms action. marrow mesenchymal stromal cells isolated from mice cultured for following experiments. After adipogenic induction, Oil Red O, alizarin red S, alkaline phosphatase staining solutions applied confirm formation lipid droplets calcium nodules. Western blotting analyses, real-time reverse transcription PCR assays, luciferase reporter performed relative RNA protein expressions activities transfected cells. pull-down immunoprecipitation assays also carried verify interaction between miR-22-5p. Additionally, mouse model closed femoral fractures was generated evaluate vivo effect increased on healing. enhanced activity osteoblasts. Overexpression miR-22-5p reversed osteopromoting lncTUG1. knockdown Anxa8 inhibitors, indicating an Upregulation could promote vivo. In conclusion, highlights functional importance recovery.

Язык: Английский

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