Modulatory role of exogenous arachidonic acid in periodontitis with type 2 diabetes mellitus mice DOI Creative Commons
Xiaomin Chen,

Yeqing He,

Yuxi Zhou

и другие.

BMC Oral Health, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 19, 2025

Type 2 diabetes mellitus (T2DM) exhibits a bidirectional relationship with periodontitis, wherein each condition influences the progression of other. Arachidonic acid (AA) exerts an anti-diabetic effect, while showing protective effect by regulating inflammatory response independently its metabolites. However, impact on periodontitis T2DM remains poorly understood. The mouse model was established combining high-sugar and high-fat diet streptozotocin injection, followed silk ligation to induce periodontitis. Alterations in diabetes-associated symptoms were evaluated. Micro-computed tomography used measure bone-related parameters, including distance from cementoenamel junction alveolar bone crest, volume/total volume mineral density. Targeted metabolomics analysis conducted evaluate exogenous AA serum metabolite levels mice type diabetic 16S rRNA gene sequencing utilized analyze microbial diversity. activity osteoclasts, factors expression related osteoclasts investigated using TRAP staining real-time quantitative PCR. successfully established. Following two weeks treatment, reduction fasting blood glucose observed mice. Exogenous alleviated loss it had no substantial contents AA-targeted reduced Staphylococcus subgingival flora mice, but significant community structure or Furthermore, decreased number increased IL-10 mRNA gingival tissue. may alleviate reducing increasing periodontal tissues, rather than change metabolites composition diversity microorganisms plaque. These findings provide potential therapeutic approach for prevention treatment T2DM.

Язык: Английский

Biodegradable ultrahigh-purity magnesium and its alloy ZX00 promote osteogenesis in the medullary cavity and glycogenolysis in the liver DOI Creative Commons
Begüm Okutan, Uwe Yacine Schwarze, Hansjörg Habisch

и другие.

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Magnesium (Mg)-based implants have become an attractive alternative to conventional permanent in the orthopedic field. While biocompatibility, degradation kinetics, and osseointegration of Mg-based been mostly investigated, impact products on bone remodeling potential systemic effects remains unclear. The aim this study was evaluate early mid-term local tissue responses degrading ultrahigh-purity ZX00 (Mg-Zn-Ca alloy) Mg (XHP-Mg) pins a juvenile healthy rat model. differences between implant types (degradable vs. permanent), implantation, age-related changes were investigated using titanium (Ti), sham-operated, control groups (non-intervention), respectively. Degradation XHP-Mg promote osteogenesis medullary cavity by upregulating expression levels Bmp2 Opg within 14 days post-surgery. higher rate resulted accumulation starting from day 3 upregulation different genes, particularly Ccl2 Cepbp. Besides good new formation, we found more parallel hydroxyapatite/collagen orientation along perimeter region compared Ti pins. In liver, reduced glycogen indicated that glycogenolysis, while only group showed serum glucagon level 14. Results suggest stimulate mainly via glycogenolysis genes metabolites. STATEMENT OF SIGNIFICANCE: Bioresorbable magnesium are promising candidates for interventions. Until now, few vivo studies explored how modulate responses. Herein, results demonstrate i) has crucial effect regulating cavity, ii) HAp/collagen matrix pattern group, iii) both liver. Our findings highlight dual role metabolic modulation. Nevertheless, is first report interaction liver metabolism.

Язык: Английский

Процитировано

0

Causal inference study of plasma proteins and blood metabolites mediating the effect of obesity-related indicators on osteoporosis DOI Creative Commons

Maomao Huang,

Fei Xing, Yue Hu

и другие.

Frontiers in Endocrinology, Год журнала: 2025, Номер 16

Опубликована: Фев. 18, 2025

Osteoporosis and obesity are both major global public health problems. Observational studies have found that osteoporosis might be related to obesity. Mendelian randomization (MR) analysis could overcome the limitations of observational in assessing causal relationships. This study aims evaluate potential relationship between obesity-related indicators by using a two-sample MR identify mediators. A total 53 indicators, 3,282 plasma protein lists, 452 blood metabolite lists were downloaded from data set as instrumental variables, GWAS MRC IEU Open database was used outcome indicators. Using univariate MR, multivariate intermediate mediating factors identified. The IVW model results show 31 may significant with (P < 0.05), except for waist circumference (id: Ieu-a-71, OR = 1.00566); remaining 30 reduce risk (OR: 0.983-0.996). 25 10 them, such ANKED46, KLRF1, LPO, CA9 protective effect on 0.996-0.999), while other 15 ATP1B1, zinc finger 175, increase 1.001-1.004). For alanine Met a-469, OR: 1.071), six including uridine 1-linoleoylglycerophosphoethanolaminecan 0.05, 0.961-0.992). direction is all correct; heterogeneity not affected horizontal pleiotropy. mediated analysis, it against histone-lysine N-methyltransferase proteins metabolites. Obesity confer osteoporosis, potentially EHMT2 Further empirical research required fully elucidate mechanisms behind influence osteoporosis. Interventions controlling adverse effects associated require further research.

Язык: Английский

Процитировано

0

Modulatory role of exogenous arachidonic acid in periodontitis with type 2 diabetes mellitus mice DOI Creative Commons
Xiaomin Chen,

Yeqing He,

Yuxi Zhou

и другие.

BMC Oral Health, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 19, 2025

Type 2 diabetes mellitus (T2DM) exhibits a bidirectional relationship with periodontitis, wherein each condition influences the progression of other. Arachidonic acid (AA) exerts an anti-diabetic effect, while showing protective effect by regulating inflammatory response independently its metabolites. However, impact on periodontitis T2DM remains poorly understood. The mouse model was established combining high-sugar and high-fat diet streptozotocin injection, followed silk ligation to induce periodontitis. Alterations in diabetes-associated symptoms were evaluated. Micro-computed tomography used measure bone-related parameters, including distance from cementoenamel junction alveolar bone crest, volume/total volume mineral density. Targeted metabolomics analysis conducted evaluate exogenous AA serum metabolite levels mice type diabetic 16S rRNA gene sequencing utilized analyze microbial diversity. activity osteoclasts, factors expression related osteoclasts investigated using TRAP staining real-time quantitative PCR. successfully established. Following two weeks treatment, reduction fasting blood glucose observed mice. Exogenous alleviated loss it had no substantial contents AA-targeted reduced Staphylococcus subgingival flora mice, but significant community structure or Furthermore, decreased number increased IL-10 mRNA gingival tissue. may alleviate reducing increasing periodontal tissues, rather than change metabolites composition diversity microorganisms plaque. These findings provide potential therapeutic approach for prevention treatment T2DM.

Язык: Английский

Процитировано

0