Poultry Science,
Год журнала:
2024,
Номер
103(5), С. 103621 - 103621
Опубликована: Март 6, 2024
In
the
large
poultry
industry,
where
farmed
chickens
are
fed
at
high
density,
prevalence
of
pathogens
and
repeated
vaccinations
induce
immune
stress,
which
can
significantly
decrease
production
performance
increase
mortality.
This
study
was
designed
to
shed
light
on
molecular
mechanisms
metabolic
pathways
involved
in
stress
through
an
in-depth
analysis
transcriptomic
metabolomic
changes
jejunum
samples
from
broilers.
Two
groups
were
established
for
experiment:
a
control
group
LPS
group.
received
intraperitoneal
injection
solution
dose
250
μg
per
kg
12,
14,
33,
35
days
age,
whereas
sterile
saline
injection.
The
severity
assessed
using
Disease
Activity
Index.
A
jejunal
section
collected
measure
intestinal
villus
structure
(villus
length
crypt
depth).
RNA
sequencing
metabolomics
data
conducted
reveal
differentially
expressed
genes
metabolites.
results
showed
that
DAI
index
increased
height/crypt
depth
decreased
total
96
672
accumulating
metabolites
detected
by
compared
comprehensive
23
enriched
appetite,
nutrient
absorption,
energy
substance
metabolism
disorders
ferroptosis
play
important
role
Our
findings
provide
deeper
understanding
responses
broilers
LPS-induced
suggesting
potential
targets
therapeutic
strategies
improve
broiler
chickens.
ACS Chemical Neuroscience,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 23, 2025
Background:
Parkinson's
disease
(PD)
is
a
neurodegenerative
disorder
characterized
by
the
involvement
of
ferroptosis
in
its
pathological
mechanism.
In
this
study,
effects
and
mechanism
BRCA1-associated
protein
1
(BAP1)
on
neuronal
PD
were
evaluated.
Methods:
A
mouse
model
was
constructed
injecting
mice
with
MPTP.
Nissl
staining,
immunohistochemistry,
immunofluorescence,
Prussian
blue
staining
evaluated
histopathology
iron
distribution.
The
cell
subjecting
SK-N-SH
cells
to
MPP+.
m6A
level
BAP1
assessed
MeRIP.
mRNA
levels
BAP1,
FTO,
IGF2BP1,
METTL3,
YTHDF2,
SLC7A11
utilizing
RT-qPCR.
Protein
SLC7A11,
p53
measured
Western
blot.
Cell
viability
using
CCK-8
assay,
TUNEL
used
for
assessing
apoptosis.
MDA,
GSH,
SOD,
Fe2+
also
measured.
interactions
among
molecules
verified
RIP
dual
luciferase
reporter
ChIP
assay.
Results:
treated
MPP+
showed
decrease
overall
BAP1.
FTO
facilitated
demethylation
leading
an
increased
expression
m6A-binding
protein,
YTHDF2
recognized
decayed
methylated
reduced
stability.
FTO/BAP1
axis
promoted
MPP+-induced
suppressing
SLC7A11.
collaboration
p53,
Knocking
down
mitigated
MPTP
model.
Conclusion:
m6A-mediated
modification
regulates
cooperating
Thus,
may
be
potential
therapeutic
target
treatment.
Biomedicine & Pharmacotherapy,
Год журнала:
2023,
Номер
164, С. 114925 - 114925
Опубликована: Май 24, 2023
Calcium
oxalate
(CaOx)
stones
are
among
the
most
common
types
of
kidney
and
associated
with
renal
tubular
damage,
interstitial
fibrosis,
chronic
disease.
The
mechanism
CaOx
crystal-induced
fibrosis
remains
unknown.
Ferroptosis,
a
type
regulated
cell
death,
is
characterised
by
iron-dependent
lipid
peroxidation,
tumour
suppressor
p53
key
regulator
ferroptosis.
In
present
study,
our
results
demonstrated
that
ferroptosis
was
significantly
activated
in
patients
nephrolithiasis
hyperoxaluric
mice
as
well
verified
protective
effects
inhibition
on
fibrosis.
Moreover,
single-cell
sequencing
database,
RNA-sequencing,
western
blot
analysis
revealed
expression
increased
disease
oxalate-stimulated
human
epithelial
line,
HK-2.
Additionally,
acetylation
enhanced
stimulation
HK-2
cells.
Mechanistically,
we
found
induction
deacetylation,
owing
to
either
SRT1720-induced
activation
deacetylase
sirtuin
1
or
triple
mutation
p53,
inhibited
alleviated
caused
crystals.
We
conclude
one
critical
mechanisms
contributing
pharmacological
via
1-mediated
deacetylation
may
be
potential
target
for
preventing
nephrolithiasis.
Poultry Science,
Год журнала:
2024,
Номер
103(5), С. 103621 - 103621
Опубликована: Март 6, 2024
In
the
large
poultry
industry,
where
farmed
chickens
are
fed
at
high
density,
prevalence
of
pathogens
and
repeated
vaccinations
induce
immune
stress,
which
can
significantly
decrease
production
performance
increase
mortality.
This
study
was
designed
to
shed
light
on
molecular
mechanisms
metabolic
pathways
involved
in
stress
through
an
in-depth
analysis
transcriptomic
metabolomic
changes
jejunum
samples
from
broilers.
Two
groups
were
established
for
experiment:
a
control
group
LPS
group.
received
intraperitoneal
injection
solution
dose
250
μg
per
kg
12,
14,
33,
35
days
age,
whereas
sterile
saline
injection.
The
severity
assessed
using
Disease
Activity
Index.
A
jejunal
section
collected
measure
intestinal
villus
structure
(villus
length
crypt
depth).
RNA
sequencing
metabolomics
data
conducted
reveal
differentially
expressed
genes
metabolites.
results
showed
that
DAI
index
increased
height/crypt
depth
decreased
total
96
672
accumulating
metabolites
detected
by
compared
comprehensive
23
enriched
appetite,
nutrient
absorption,
energy
substance
metabolism
disorders
ferroptosis
play
important
role
Our
findings
provide
deeper
understanding
responses
broilers
LPS-induced
suggesting
potential
targets
therapeutic
strategies
improve
broiler
chickens.
