The Impact of Obesity on Autophagy in Human Adipose-Derived Mesenchymal Stromal Cells
Xing Li,
Ronscardy Mondesir,
Logan M. Glasstetter
и другие.
Cell Transplantation,
Год журнала:
2025,
Номер
34
Опубликована: Март 1, 2025
Mesenchymal
stromal
cells
(MSCs)
possess
therapeutic
properties,
which
can
be
blunted
by
obesity.
Autophagy,
a
cellular
recycling
process,
is
essential
for
MSC
function.
We
investigated
the
mechanisms
obesity
affects
properties
of
MSCs,
with
focus
on
autophagy.
Adipose
tissue
was
obtained
from
kidney
donors
[body
mass
index
(BMI)
<30
kg/m
2
,
non-obese]
or
individuals
undergoing
weight
loss
surgery
(BMI
≥30
obese)
harvesting
(
n
=
11
each);
samples
were
randomized
to
sequencing
(seq;
5
each)
functional
studies
6
each).
MSCs
sequenced
determine
their
epigenetic
(5-hydroxymethylcytosine)
and
transcriptomic
profiles
across
autophagy-related
genes
using
hydroxymethylated
DNA
immunoprecipitation
mRNA-seq,
respectively.
Genes
shared
trends
in
both
datasets
underwent
Reverse
Transcription
Quantitative
Polymerase
Chain
Reaction
(RT-qPCR)
validation.
During
studies,
2-h
starvation
used
induce
autophagy
vitro
enabling
detection
changes
protein
expression
microtubule-associated
1A/1B-light
chain-3
autophagic
flux.
Obesity
amplified
starvation-induced
reduction
flux
while
promoting
earlier
generation
new
autophagosomes
during
initiation.
Integrated
analysis
two
revealed
124
differentially
30
expressed
mRNAs.
Among
six
overlapping
genes,
three
exhibited
same-direction
trends.
Of
these,
STX12
SLC25A4
may
implicated
impact
MSCs.
Therefore,
human
alter
adipose
tissue–derived
MSC,
thereby
metabolism
Язык: Английский
Understanding the Link Between Sterol Regulatory Element Binding Protein (SREBPs) and Metabolic Dysfunction Associated Steatotic Liver Disease (MASLD)
Current Obesity Reports,
Год журнала:
2025,
Номер
14(1)
Опубликована: Апрель 14, 2025
Язык: Английский
The functional significance of vascular DNA hypermethylation in atherosclerosis: a historical perspective
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 15, 2025
A
decade
ago,
independent
mechanistic
and
descriptive
epigenomics
data
demonstrated
for
the
first
time
that
vascular
DNA
hypermethylation
is
a
landmark
of
causal
factor
in
human
murine
atherosclerosis.
Since
then,
flurry
converging
evidence
has
assigned
prominent
role
to
across
natural
history
cardiovascular
disease
(CVD),
from
exposure
risk
factors,
onset
progression
atheroma.
induced
by
mediates
metabolic
outcomes
high-fat
diets
CVD
risk-enhancing
lipids
several
models.
Early-stage
atheroma
hypermethylated
compared
normal
adjacent
tissue,
trend
amplified
as
progresses.
That
resulted
strong
interest
epigenetic
drugs
CVD.
Crucially,
methylation
inhibitor
azacytidine
been
singled
out
potent
guardian
contractile,
anti-atherogenic
phenotype
smooth
muscle
cells
(SMC).
Those
findings
are
gaining
relevance,
antiatherogenic
effects
anticancer
decitabine
fit
into
recently
revived
hypothesis
SMC-driven
cancer-like
mass.
Finally,
this
10-year
anniversary
marked
report
nanoparticles
loaded
with
methyltransferase
drug
anti-inflammatory
inhibit
Exciting
work
lies
ahead
assess
whether
practical
effective
target
prevent
or
cure
Язык: Английский
Senescence in Adipose-Derived Stem Cells: Biological Mechanisms and Therapeutic Challenges
Опубликована: Июль 2, 2024
Adipose
tissue-derived
stem
cells
(ADSCs)
represent
a
subset
of
mesenchymal
in
every
adipose
compartment
throughout
the
body.
ADSCs
can
differentiate
into
various
cell
types,
including
chondrocytes,
osteocytes,
myocytes,
and
adipocytes.
Moreover,
they
exhibit
notable
potential
to
vitro
from
other
germinal
lineages,
endothelial
neurons.
have
wide
range
clinical
applications,
breast
surgery
chronic
wounds.
Furthermore,
are
promising
population
for
future
tissue-engineering
uses.
Accumulating
evidence
indicates
decreased
proliferation
differentiation
with
increasing
age,
body
mass
index,
diabetes
mellitus,
metabolic
syndrome,
or
exposure
radiotherapy.
Therefore,
recent
literature
thoroughly
investigates
this
population's
senescence
mechanisms
how
hinder
their
possible
therapeutic
applications.
This
review
will
discuss
biological
physio-pathological
causes
behind
ADSC
impact
cellular
functionality.
we
examine
strategies
invert
these
processes,
re-establishing
full
regenerative
progenitor
population.
Язык: Английский
Senescence in Adipose-Derived Stem Cells: Biological Mechanisms and Therapeutic Challenges
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(15), С. 8390 - 8390
Опубликована: Авг. 1, 2024
Adipose
tissue-derived
stem
cells
(ADSCs)
represent
a
subset
of
the
mesenchymal
in
every
adipose
compartment
throughout
body.
ADSCs
can
differentiate
into
various
cell
types,
including
chondrocytes,
osteocytes,
myocytes,
and
adipocytes.
Moreover,
they
exhibit
notable
potential
to
vitro
from
other
germinal
lineages,
endothelial
neurons.
have
wide
range
clinical
applications,
breast
surgery
chronic
wounds.
Furthermore,
are
promising
population
for
future
tissue-engineering
uses.
Accumulating
evidence
indicates
decreased
proliferation
differentiation
with
an
increasing
age,
body
mass
index,
diabetes
mellitus,
metabolic
syndrome,
or
exposure
radiotherapy.
Therefore,
recent
literature
thoroughly
investigates
this
population’s
senescence
mechanisms
how
hinder
its
possible
therapeutic
applications.
This
review
will
discuss
biological
physio-pathological
causes
behind
ADSC
impact
cellular
functionality.
we
examine
strategies
invert
these
processes,
re-establishing
full
regenerative
progenitor
population.
Язык: Английский
Adipose Tissue Plasticity: A Comprehensive Definition and Multidimensional Insight
Biomolecules,
Год журнала:
2024,
Номер
14(10), С. 1223 - 1223
Опубликована: Сен. 27, 2024
Adipose
tissue
is
composed
of
adipocytes,
stromal
vascular
fraction,
nerves,
surrounding
immune
cells,
and
the
extracellular
matrix.
Under
various
physiological
or
pathological
conditions,
adipose
shifts
cellular
composition,
lipid
storage,
organelle
dynamics
to
respond
stress;
this
remodeling
called
"adipose
plasticity".
plasticity
includes
changes
in
size,
species,
number,
storage
capacity,
differentiation
function
as
well
alterations
distribution
composition
tissue.
This
has
a
major
role
growth,
obesity,
organismal
protection,
internal
environmental
homeostasis.
Moreover,
certain
thresholds
exist
for
with
significant
individualized
differences.
Here,
we
comprehensively
elaborate
on
specific
connotation
relationship
between
development
many
diseases.
Meanwhile,
summarize
possible
strategies
treating
obesity
response
plasticity,
intending
provide
new
insights
into
dynamic
contribute
ideas
relevant
clinical
problems.
Язык: Английский