Infinite Coordination Polymer Polydopamine Nanocomposites Dual‐Pathway Multistep Induction of Long‐Term Hyperimmunity Combined With Photothermal‐Chemo Synergistic Therapy Colorectal Cancer

Aggregate, Год журнала: 2025, Номер unknown

Опубликована: Янв. 5, 2025

ABSTRACT To improve the long‐term therapeutic efficacy of colorectal cancer, we propose a synergistic treatment strategy involving dual‐pathway, multistep induction hyperimmunity combined with photothermal‐chemotherapy. implement this strategy, infinite coordination polymer nanoparticles (SN38‐Mn(II)‐EGCG ICP NPs) were prepared by coordinating SN38, EGCG, and Mn 2+ . These then coated polydopamine (PDA) grafted folate‐PEG‐thiol (FA‐PEG‐SH) onto their surfaces, producing tumor‐targeting folate‐modified PDA nanocomposites (ICP@FA‐PDA nanocomposites). exhibit particle size 94.9 ± 1.6 nm high drug loading capacity (83.3% 1.5%), release under acidic conditions while maintaining stability in physiological environments. Furthermore, each component within serves multiple functions. Notably, incorporation components triggers powerful antitumor immune effect establishes enduring memory through dual‐pathway approach, which is produced activation cGAS‐STING pathway immunogenic cell death (ICD) four‐component process. Under low‐dose regimen, approach induces generates ultra‐long immunological memory, marked ninefold increase CD8 + T infiltration, fourfold CD4 lymphocytes, reduction Treg cells, fivefold cells. The remarkable therapy achieved combination SN38 EGCG chemotherapy photothermal therapy. In vivo studies demonstrated that mice treated ICP@FA‐PDA complete eradication cancer 21 days, no recurrence observed 60 days. hold significant promise potential for future clinical translation.

Язык: Английский

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Leveraging nature’s nanocarriers: Translating insights from extracellular vesicles to biomimetic synthetic vesicles for biomedical applications

Science Advances, Год журнала: 2025, Номер 11(9)

Опубликована: Фев. 26, 2025

Naturally occurring extracellular vesicles (EVs) and synthetic nanoparticles like liposomes have revolutionized precision diagnostics medicine. EVs excel in biocompatibility cell targeting, while offer enhanced drug loading capacity scalability. The clinical translation of is hindered by challenges including low yield heterogeneity, whereas face rapid immune clearance limited targeting efficiency. To bridge these gaps, biomimetic (SVs) emerged as innovative platforms, combining the advantageous properties liposomes. This review emphasizes critical aspects EV biology, such mechanisms EV-cell interaction source-dependent functionalities modulation, tissue regeneration, informing SV engineering. We reviewed a broad array SVs, with focus on lipid bilayered functionalized proteins. These include cell-derived nanovesicles, protein-functionalized liposomes, hybrid vesicles. By addressing current highlighting opportunities, this aims to advance SVs for transformative biomedical applications.

Язык: Английский

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Surface Components and Biological Interactions of Extracellular Vesicles

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

Extracellular vesicles (EVs) are critical mediators of intercellular communication, carrying bioactive cargo and displaying diverse surface components that reflect their cellular origins functions. The EV surface, composed proteins, lipids, glycocalyx elements, plays a pivotal role in targeting recipient cells, mediating biological interactions, enabling selective delivery. This review comprehensively examined the molecular architecture surfaces, linking biogenesis to functional diversity, highlights therapeutic diagnostic potential diseases such as cancer cardiovascular disorders. Additionally, we explore emerging applications EVs, including machine-learning-assisted analysis, chemical integration, cross-system combinations. also discusses some key challenges clinical translation EV-related technologies.

Язык: Английский

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Exploring RNA cargo in extracellular vesicles for pleural mesothelioma detection

BMC Cancer, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 7, 2025

Pleural Mesothelioma (PM) is a highly aggressive cancer, for which effective early detection remains challenge due to limited screening options and low sensitivity of biomarkers discovered so far. While extracellular vesicles (EVs) have emerged as promising candidates blood-based biomarkers, their role in PM has not been studied yet. In this study, we characterized the transcriptomic profile EVs secreted by primary cells explored potential biomarker source detection. We collected cell culture supernatant from early-passage cultures derived pleural effusion 4 patients. were isolated using Qiagen exoEasy Maxi kit. RNA isolation was done mirVana PARIS Finally, single-end sequencing with Illumina Novaseq 6000. identified range species expressed cells, including protein-coding (80%), long non-coding (13%), pseudogenes (4.5%), short (1.6%). detected subset genes associated previously epithelioid (32 genes) sarcomatoid molecular components (36 PM-EVs. To investigate whether these markers could serve blood, compared content PM-EVs cargo plasma healthy donors (publicly available data). Majority upregulated RNAs. Interestingly, 25 them marker genes. functional analysis revealed that PM-EV Epithelial-Mesenchymal transition, glycolysis, hypoxia. This first study characterize cultures, demonstrating Further investigation will provide new insights into disease biology therapeutic avenues.

Язык: Английский

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A synthetic model of bioinspired liposomes to study cancer-cell derived extracellular vesicles and their uptake by recipient cells

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 11, 2025

Extracellular vesicles (EVs) are secreted by most cell types and play a central role in cell-cell communication. These naturally occurring nanoparticles have been particularly implicated cancer, but EV heterogeneity lengthy isolation methods with low yield make them difficult to study. To circumvent the challenges research, we aimed develop unique synthetic model engineering bioinspired liposomes study properties their impact on cellular uptake. We produced EV-like mimicking physicochemical as cancer EVs. First, using panel of non-cancer lines, small EVs were isolated ultracentrifugation characterized dynamic light scattering (DLS) nanoparticle tracking analysis (NTA). Cancer ranged mean size from 107.9 161 nm NTA, hydrodynamic diameter 152 355 DLS, zeta potential ranging − 25 -6 mV. markers TSG101 CD81 positive all Using microfluidics bottom-up approach, nanoprecipitation method adapted micromixers developed our group. A library liposome formulations was created that mimicked ranges (90–222 nm) (anionic [-47 mV] neutral [-1 mV]) at production throughput up 41 mL/h yielding concentration 1 × 1012 particles per mL. reproduced controlling flow conditions lipid composition set statistical based response surface methodology. The fairly accurate an R-squared > 70% for both parameters between targeted obtained liposomes. Finally, internalization fluorescently labeled assessed confocal microscopy cytometry, correlated decreasing less negative potential, providing insights into effects key properties. Our data demonstrated can be used powerful By cell-derived properties, individually combination. Taken together, present novel system accelerate research models.

Язык: Английский

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Circulating tumor DNA to monitor treatment response in solid tumors and advance precision oncology

npj Precision Oncology, Год журнала: 2025, Номер 9(1)

Опубликована: Март 24, 2025

Circulating tumor DNA (ctDNA) has emerged as a dynamic biomarker in cancer, evidenced by its increasing integration into clinical practice. Carrying specific characteristics, ctDNA can be used to inform treatment selection, monitor response, and identify drug resistance. In this review, we provide comprehensive, up-to-date summary of monitoring response with focus on lung, colorectal, breast cancers, discuss current challenges future directions.

Язык: Английский

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Possible role of human ribonuclease dicer in the regulation of R loops

FEBS Open Bio, Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

R loops are three-stranded nucleic acid structures that form naturally in cells under various conditions, mainly as intermediates during replication or by-products transcription. involved the regulation of many important cellular processes, including replication, transcription, centromere stabilization, protection chromosome ends, control telomere length. Unscheduled linked to diseases, cancer, neurodegenerative, inflammatory disorders. The list cancer diseases excessive loop accumulation is growing rapidly. There currently much debate about understanding abnormal formation and its impact on genome instability development. In this review, we briefly describe nature loops, their physiological pathological proteins loops. addition, emphasize possible role human ribonuclease Dicer, a multi-tasking protein mostly known for microRNA biogenesis, We also discuss involvement development potential use diagnostic biomarkers. Knowledge molecular mechanisms underlying dysregulation may significantly improve our biology provide new directions research.

Язык: Английский

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Recent Advances in Bioreceptor-based Sensing for Extracellular Vesicle Analysis

Biosensors and Bioelectronics, Год журнала: 2025, Номер unknown, С. 117432 - 117432

Опубликована: Апрель 1, 2025

Язык: Английский

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Extracellular vesicles as a source of biomarkers in hematological malignancies: looking toward clinical appilcations

Expert Review of Molecular Diagnostics, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

Extracellular vesicles are membranous particles released by cells in physiological and pathological conditions. Their cargo is heterogeneous since it includes different biomolecules such as nucleic acids proteins. Oncogenic alterations affect the composition of extracellular model their content during cancer evolution. This review provides an overview studies focused on source biomarkers hematological malignancies. A special insight into vesicles-derived tools for evaluating prognosis malignancies response to treatment given. a valuable However, translation from bench bedside challenged lack standardization preanalytical variables experimental workflow. The release standard operating procedures validation large cohort patients will help exploiting potential clinical setting.

Язык: Английский

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Cell-Free DNA: Features and Attributes Shaping the Next Frontier in Liquid Biopsy

Molecular Diagnosis & Therapy, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

Cell-free DNA (cfDNA) is changing the face of liquid biopsy as a minimally invasive tool for disease detection and monitoring, with its main applications in oncology prenatal testing, rising roles transplant patient monitoring. However, processes cfDNA biogenesis, fragmentation, clearance are complex require further investigation. Evidence suggests that production relates to mechanisms cell death repair, both which influence fragment size applicability biomarker. An emerging domain, fragmentomics being explored advancing field diagnostics using non-mutational signatures such ratios methylation patterns. Thus, this review examines structural diversity various sizes. In examining these subsets, we discuss their distinct biological origins potential clinical utility. Development sequencing methodologies has broadened application diagnosing cancers organ-specific pathologies, well directing personalized therapies. This been achieved by identifying uncovering different subsets biofluids biofluids. Different provide important insights regarding genomic epigenetic features, enhancing understanding gene regulation, tissue-specific functions, progression. Advancement key areas asserts increasing relevance use Continued exploration expected drive innovation integration into routine practice.

Язык: Английский

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