Investigating Short-Chain Fatty Acids Effects on Extracellular Vesicles Production in Colorectal Cancer DOI Creative Commons
Daniela O. Macedo, Sara Abalde‐Cela, Lorena Diéguez

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 2, 2024

Abstract Colorectal cancer (CRC) is the third most diagnosed and second leading cause of cancer-related deaths globally, often due to late detection limited treatment options. Recent studies have linked alterations in gut microbiota CRC, particularly emphasizing role short-chain fatty acids (SCFAs) like acetate, propionate, butyrate shaping tumor microenvironment (TME). SCFAs contribute CRC pathogenesis by inducing lysosomal membrane permeabilization, cell cycle arrest, apoptosis cells. Extracellular vesicles (EVs) are membrane-bound that facilitate intercellular communication gained attention as promising non-invasive biomarkers for diagnosis monitoring. EVs participate cellular response mechanisms external stimuli transferring proteins, lipids, nucleic between cells, thus modulating target behavior promoting coordinated responses stress environmental challenges. This process essential adaptation plays a significant pathophysiological processes, including progression immune modulation, making highly relevant clinical research. study examined impact on EV production phenotype The results indicated notable increase EV-sized particles following SCFA colorectal lines, SW480 line. For while co-precipitated protein levels remained stable, there was slight decrease DNA an EV-associated DNA. KRAS-mutant cells exhibited pronounced response, their heightened sensitivity SCFA. Notably, microsatellite instability - key biomarker immunotherapy detected both small large populations from BRAF-mutant RKO after treatment, even at low concentrations. These findings underscore potential molecular markers, paving way further exploration precision oncology. Figure Graphical Abstract. Effects characteristics Treatment with led number particles, corresponding EV-DNA. also identified MSI s-EV L-EV, Created using BioRender.

Язык: Английский

Analyzing Extracellular Vesicle‐associated DNA Using Transmission Electron Microscopy at the Single EV‐level DOI Creative Commons
Thupten Tsering, Amélie Nadeau, Janusz Rak

и другие.

Current Protocols, Год журнала: 2024, Номер 4(11)

Опубликована: Ноя. 1, 2024

Abstract Extracellular vesicles (EVs) play an important role in cell‐cell communication, carrying bioactive molecules including DNA. EV‐associated DNA (EV‐DNA) has created enormous interest the field of biomarkers, particularly related to liquid biopsy. However, its analysis is challenging due nanoscale structure EVs, low abundance EV‐DNA, and surrounding biogenetic debate. Therefore, novel protocols enhance accurate detection EV‐DNA are essential study normal physiology disease states. Here, we provide two for confirming presence from biological samples. In first protocol, ultrathin sectioning EVs combined with immunogold labeling detect double‐stranded (ds) within EV lumen using transmission electron microscopy (TEM). second whole‐mount allows detailed morphological their surface‐associated Using TEM imaging, have demonstrated that cancer‐cell‐derived individual exhibit simultaneous positivity dsDNA surface protein tetraspanin 9. We believe this method can be used label any proteins inside as well on EVs. This aid characterization single identification verification biomarkers. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1 : isolation cell‐culture‐conditioned medium, embedding, sectioning, labeling, imaging 2 Whole‐mount immunolabeling

Язык: Английский

Процитировано

1
Molecular Profiling of Endocrine Resistance in HR+/HER2-Metastatic Breast Cancer: Insights from Extracellular Vesicles-Derived DNA and ctDNA in Liquid Biopsies DOI Open Access
Ana M. Martínez-Rodríguez, Jesús Fuentes‐Antrás, Víctor Lorca

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 13045 - 13045

Опубликована: Дек. 4, 2024

Standard treatments in hormone receptor-positive (HR+)/HER2-metastatic breast cancer (mBC) typically involve endocrine therapy (ET) combined with CDK4/6 inhibitors, yet resistance to ET remains a persistent challenge advanced cases. A deeper knowledge of the use liquid biopsy is crucial for implementation precision medicine mBC real-time treatment guidance. Our study assesses prognostic value

Язык: Английский

Процитировано

1
Investigating Short-Chain Fatty Acids Effects on Extracellular Vesicles Production in Colorectal Cancer DOI Creative Commons
Daniela O. Macedo, Sara Abalde‐Cela, Lorena Diéguez

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 2, 2024

Abstract Colorectal cancer (CRC) is the third most diagnosed and second leading cause of cancer-related deaths globally, often due to late detection limited treatment options. Recent studies have linked alterations in gut microbiota CRC, particularly emphasizing role short-chain fatty acids (SCFAs) like acetate, propionate, butyrate shaping tumor microenvironment (TME). SCFAs contribute CRC pathogenesis by inducing lysosomal membrane permeabilization, cell cycle arrest, apoptosis cells. Extracellular vesicles (EVs) are membrane-bound that facilitate intercellular communication gained attention as promising non-invasive biomarkers for diagnosis monitoring. EVs participate cellular response mechanisms external stimuli transferring proteins, lipids, nucleic between cells, thus modulating target behavior promoting coordinated responses stress environmental challenges. This process essential adaptation plays a significant pathophysiological processes, including progression immune modulation, making highly relevant clinical research. study examined impact on EV production phenotype The results indicated notable increase EV-sized particles following SCFA colorectal lines, SW480 line. For while co-precipitated protein levels remained stable, there was slight decrease DNA an EV-associated DNA. KRAS-mutant cells exhibited pronounced response, their heightened sensitivity SCFA. Notably, microsatellite instability - key biomarker immunotherapy detected both small large populations from BRAF-mutant RKO after treatment, even at low concentrations. These findings underscore potential molecular markers, paving way further exploration precision oncology. Figure Graphical Abstract. Effects characteristics Treatment with led number particles, corresponding EV-DNA. also identified MSI s-EV L-EV, Created using BioRender.

Язык: Английский

Процитировано

0