Mechanistic insights into CDCA gene family-mediated glioblastoma progression: implications for diagnosis, prognosis, and therapeutic targeting DOI Creative Commons
Chang Liu

Hereditas, Год журнала: 2025, Номер 162(1)

Опубликована: Март 20, 2025

Abstract Background Glioblastoma (GBM) is a highly aggressive brain tumor characterized by poor prognosis and limited therapeutic options. Understanding the molecular mechanisms driving GBM progression essential for developing more effective diagnostic approaches. Specifically, investigating Cell Division Cycle-Associated (CDCA) genes offers new perspectives on cell cycle regulation proliferation of cells, which are key factors in growth resistance to treatment. These have not been extensively studied GBM, making them promising area targeted research potential interventions. This project was launched elucidate pathogenic, diagnostic, roles CDCA GBM. Methodology Total RNA extracted from lines followed RT-qPCR analyze expression genes. The validation, prognostic significance, mutational analysis were performed using various databases. Functional assays, including gene knockdown, colony formation, proliferation, wound healing, conducted U87MG cells assess role CDCA7 CDCA8 Results 12 6 normal revealed significant overexpression these ROC curve demonstrated excellent potential, with AUC values 1 most indicates that effectively distinguishes cells. Validation additional TCGA data confirmed upregulation tumors, association cancer-related pathways. Survival showed higher correlated patients. Mutation, CNV, methylation analyses alterations genes, further supporting their Additionally, linked immune modulation cycle-related functions, suggesting involvement evasion proliferation. Knockdown experiments reduction migration, highlighting as targets. Conclusion Overall, our findings suggest could serve both biomarkers targets

Язык: Английский

Extracellular vesicle biomarkers redefine prostate cancer radiotherapy DOI Creative Commons
H Chan, Qi Wang, Andrew Howie

и другие.

Cancer Letters, Год журнала: 2025, Номер unknown, С. 217568 - 217568

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Exploring the therapeutic potential of natural products in modulating miRNA networks in prostate cancer DOI
Mohamed A. Elkady

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 20, 2025

Язык: Английский

Процитировано

0
Epigenetic regulatory protein chromobox family regulates multiple signalling pathways and mechanisms in cancer DOI Creative Commons

Weiyu Su,

Weiwen Wang, Guanghui Zhang

и другие.

Clinical Epigenetics, Год журнала: 2025, Номер 17(1)

Опубликована: Март 13, 2025

Signal transduction plays a pivotal role in modulating myriad of critical processes, including the tumour microenvironment (TME), cell cycle arrest, proliferation and apoptosis cells, as well their migration, invasion, epithelial–mesenchymal transition (EMT). Epigenetic mechanisms are instrumental genesis progression tumours. The Chromobox (CBX) family proteins, which serve significant epigenetic regulators, exhibit tumour-specific expression patterns biological functionalities. These proteins influenced by multitude factors could modulate activation diverse signalling pathways within cells through alterations modifications, thereby acting either oncogenic agents or suppressors. This review aims to succinctly delineate composition, structure, function, CBXs with an emphasis on synthesizing deliberating CBXs-mediated intracellular intricate governing tumourigenesis progression. Moreover, plethora contemporary studies have substantiated that might represent promising target for diagnosis therapeutic intervention patients. We also compiled scrutinized current research landscape concerning inhibitors targeting CBXs, aspiring aid researchers gaining deeper comprehension roles malignant evolution tumours, furnish novel perspectives innovation targeted therapeutics.

Язык: Английский

Процитировано

0
Mechanistic insights into CDCA gene family-mediated glioblastoma progression: implications for diagnosis, prognosis, and therapeutic targeting DOI Creative Commons
Chang Liu

Hereditas, Год журнала: 2025, Номер 162(1)

Опубликована: Март 20, 2025

Abstract Background Glioblastoma (GBM) is a highly aggressive brain tumor characterized by poor prognosis and limited therapeutic options. Understanding the molecular mechanisms driving GBM progression essential for developing more effective diagnostic approaches. Specifically, investigating Cell Division Cycle-Associated (CDCA) genes offers new perspectives on cell cycle regulation proliferation of cells, which are key factors in growth resistance to treatment. These have not been extensively studied GBM, making them promising area targeted research potential interventions. This project was launched elucidate pathogenic, diagnostic, roles CDCA GBM. Methodology Total RNA extracted from lines followed RT-qPCR analyze expression genes. The validation, prognostic significance, mutational analysis were performed using various databases. Functional assays, including gene knockdown, colony formation, proliferation, wound healing, conducted U87MG cells assess role CDCA7 CDCA8 Results 12 6 normal revealed significant overexpression these ROC curve demonstrated excellent potential, with AUC values 1 most indicates that effectively distinguishes cells. Validation additional TCGA data confirmed upregulation tumors, association cancer-related pathways. Survival showed higher correlated patients. Mutation, CNV, methylation analyses alterations genes, further supporting their Additionally, linked immune modulation cycle-related functions, suggesting involvement evasion proliferation. Knockdown experiments reduction migration, highlighting as targets. Conclusion Overall, our findings suggest could serve both biomarkers targets

Язык: Английский

Процитировано

0