Transcriptomic and Metabolomic Analysis Reveals the Impact of Autophagy Regulation on Purine Content in Mutton DOI Creative Commons
Xu Han, Yang Chen, D. Tan

и другие.

Foods, Год журнала: 2025, Номер 14(5), С. 718 - 718

Опубликована: Фев. 20, 2025

Excessive purine intake increases the risk of hyperuricemia and gout. This study investigates relationship between content in mutton meat quality traits explores regulatory mechanism metabolism through transcriptomic metabolomic analyses. Thirty-six-month-old hybrid sheep (Australian White × Small-tail Han) were selected. Purine traits, including inosine monophosphate (IMP), intramuscular fat (IMF), shear force, pH, cooking loss, color, measured. Transcriptomic sequencing analysis performed on muscle samples with high (3895.70 ± 107.03 mg/kg) low (2751.72 175.29 contents (n = 6 per group). Differentially expressed genes validated by quantitative PCR Western blot. In vivo autophagy regulation experiments mice using rapamycin (activator) chloroquine (inhibitor). No significant correlation was found suggesting that reducing does not negatively affect quality. An autophagy-related gene, LAPTM5 (lysosomal-associated protein transmembrane 5), identified as a key gene regulating content. demonstrated affects modulating enzymes such xanthine dehydrogenase (XDH) hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT). reveals role XDH HPRT, providing new insights for improving future.

Язык: Английский

Transcriptomic and Metabolomic Analysis Reveals the Impact of Autophagy Regulation on Purine Content in Mutton DOI Creative Commons
Xu Han, Yang Chen, D. Tan

и другие.

Foods, Год журнала: 2025, Номер 14(5), С. 718 - 718

Опубликована: Фев. 20, 2025

Excessive purine intake increases the risk of hyperuricemia and gout. This study investigates relationship between content in mutton meat quality traits explores regulatory mechanism metabolism through transcriptomic metabolomic analyses. Thirty-six-month-old hybrid sheep (Australian White × Small-tail Han) were selected. Purine traits, including inosine monophosphate (IMP), intramuscular fat (IMF), shear force, pH, cooking loss, color, measured. Transcriptomic sequencing analysis performed on muscle samples with high (3895.70 ± 107.03 mg/kg) low (2751.72 175.29 contents (n = 6 per group). Differentially expressed genes validated by quantitative PCR Western blot. In vivo autophagy regulation experiments mice using rapamycin (activator) chloroquine (inhibitor). No significant correlation was found suggesting that reducing does not negatively affect quality. An autophagy-related gene, LAPTM5 (lysosomal-associated protein transmembrane 5), identified as a key gene regulating content. demonstrated affects modulating enzymes such xanthine dehydrogenase (XDH) hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT). reveals role XDH HPRT, providing new insights for improving future.

Язык: Английский

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