Electroacupuncture Attenuates Intestinal Barrier Disruption via the α7nAChR-Mediated HO-1/p38 MAPK/NF-κB Pathway in a Mouse Model of Metabolic Dysfunction-Associated Fatty Liver Disease: A Randomized, Single-Blind, Controlled Trial
Biomedicines,
Год журнала:
2025,
Номер
13(4), С. 802 - 802
Опубликована: Март 27, 2025
Background:
Gut
barrier
integrity
plays
a
crucial
role
in
the
pathogenesis
of
metabolic
dysfunction-associated
fatty
liver
disease
(MAFLD).
Electroacupuncture
(EA)
at
ST-36
can
ameliorate
inflammatory
responses
via
stimulating
α7
nicotinic
acetylcholine
receptor
(α7nAChR),
but
whether
EA
is
effective
preserving
intestinal
MAFLD
has
not
been
exactly
illustrated.
This
investigation
explored
potential
protection
mechanisms
targeting
dismantled
gut
MAFLD.
Methods:
C57BL/6
mice
were
randomly
allocated
into
several
subgroups:
control
(CON),
high-fat
diet
(HFD),
HFD
with
EA,
and
α7nAChR
inhibitor
α-BGT,
HO-1
knockout
(KO).
Body
weight,
visceral
fat
index,
histopathological
examination
intestine
determined.
Serum
biological
indexes
evaluated
through
corresponding
kits.
Furthermore,
expressions
HO-1,
α7nAChR,
barrier-associated
proteins,
molecular
tissues
assessed
Western
blot,
RT-qPCR,
immunohistology,
or
immunofluorescence
examination.
Results:
treatment
decreased
body
index
gain
mitigated
function
injury
abnormal
lipid
indexes,
exhibiting
less
severity
hepatic
steatosis,
fibrosis,
inflammation
Lower
permeability,
epithelial
disruption,
upregulation
tight
junction
proteins
after
suggested
protective
effects
attenuating
dysfunction.
These
abolished
by
α-BGT
deletion.
Mechanistically,
markedly
enriched
expression
phosphorylated
p38
MAPK/NF-κB
activation,
which
was
lost
KO
treatment.
Conclusions:
The
may
be
attributed
to
preserved
barrier,
thereby
alleviating
systemic
preventing
subsequent
hits,
where
α7nAChR-mediated
HO-1/p38
pathway
maintain
homeostasis.
Язык: Английский
Possible association between metabolic dysfunction-associated steatotic liver disease predictors and hand grip strength
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Апрель 14, 2025
Both
Metabolic
Dysfunction-Associated
Steatotic
Liver
Disease
(MASLD)
and
sarcopenia
are
associated
with
numerous
chronic
diseases,
the
link
between
two
broad-spectrum
phenotypes
has
been
extensively
researched.
We
focused
on
relationship
hepatic
steatosis
index
(HSI)
hand
grip
strength
(HGS).
The
Korean
National
Health
Nutrition
Examination
Surveys
(2014-2019)
were
utilized
to
identify
association
estimated
MASLD
(eMASLD)
muscle
strength.
HSI
determined
eMASLD
status,
HGS
evaluated
demonstrated
a
positive
correlation
HGS.
alanine
transaminase
(ALT)/aspartate
aminotransferase
(AST)
ratio
diabetes
among
five
components
of
exhibited
significant
relationships
in
men
women.
robust
linearity
was
observed
multivariate
models
stratified
analyses,
particularly
older
non-diabetic
higher
body
mass
(BMI)
young
women
without
diabetes.
However,
younger
nonlinear
associations
influenced
by
ALT/AST
ratio,
BMI,
After
adjusting
negative
observed,
disruption
Our
findings
highlight
complex
interplay
broad-range
liver
profiles
linearity.
Язык: Английский