Angiopoietin‐2 regulates the phenotypic switch of vascular smooth muscle cells DOI

Xiaowen Gan,

Shenjiao Lu,

Fen Ning

и другие.

The FASEB Journal, Год журнала: 2025, Номер 39(5)

Опубликована: Март 3, 2025

Abstract During uterine spiral artery remodeling, vascular smooth muscle cells (VSMCs) become disorganized and undergo phenotypic switching from a contractile to more synthetic phenotype. We have previously reported that natural killer induce this VSMC by secreting angiopoietin‐2 (Ang‐2). Here, we identified the specific mechanisms which Ang‐2 plays role in phenomenon. VSMCs isolated human umbilical arteries were used as an vitro model investigate of switching. Human decidua tissue preeclamptic control pregnancies was collected compare expression levels related proteins. induced phenotype evidenced decreased marker expression, increased proliferation migration, altered cytoskeleton. expressed integrin β6 interacted directly with phosphorylation FAK (S910 Y397), AKT (S473), mTOR (S2448). Knockdown recovered calponin loss resulted lower EZH2 abundance. Inhibition both attenuated Ang‐2‐induced inhibition LC3 II/LC3 I ratio ATG7 proliferation. Lipid peroxidation ferrostatin‐1 or IL‐8 receptor antagonist navarixin inhibited migration. secretion significantly lipid inhibition. In decidua, there unremodeled arteries, abundance dysregulated. dysregulation may disrupt remodeling contribute preeclampsia. be novel therapeutic target for treatment pregnancy complications affected incomplete remodeling.

Язык: Английский

Titanium nanotubes modulate immunophenotyping and cytokine secretion of T cells via IL-17A: a bioinformatic analysis and experimental validation DOI Creative Commons

Jingju Yin,

Yunyang Liao,

Shaofeng Liu

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 7, 2025

We aim to explore the immunomodulatory properties of T cells on different titanium nanotubes and key immunological factors involved in this process. Transcriptome data from GEO database healthy people implants were used analyze cell infiltration factor distribution adaptive immune using bioinformatics tools. activated rat cultured that prepared by anodization with diameters (P-0, NT15-30 nm, NT40-100 NT70-200 nm). The proliferation expressions main transcription cytokines T-cells detected. Magnetic bead sorting CD3+ transcriptome sequencing performed signaling pathways may influence related responses. Bioinformatics analysis showed peri-implant tissues enriched most T-cell subtypes. T-cell-mediated responses IL-17A. On third day, NT15 NT40 groups significantly higher pro-proliferative effects than NT70 group (P<0.05). Notably, exhibited lowest T-bet expression (P<0.05) along highest levels Rorγt, Gata3, Foxp3(P<0.05), followed group. Additionally, demonstrated reduced RANKL, TNF-α, IL-6 increased OPG IL-10 Meanwhile, had lower IFN-γ expression(P>0.05) but IL-4, TGF-β1 expressions(P<0.05). Differential expressed genes (DGEs) morphologies mostly IL-17 pathway mediated IL-17A/F. Gene protein indicated secretion IL-17A cells. Titanium nanotube medium (100 nm) small (30 sizes differentiation secretion, T-cell-derived likely playing a regulatory role.

Язык: Английский

Процитировано

0
β-elemene, a sesquiterpene constituent from Curcuma phaeocaulis inhibits the development of endometriosis by inducing ferroptosis via the MAPK and STAT3 signaling pathways DOI

Zhiyi Fu,

Hao Liu, Yehong Kuang

и другие.

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119344 - 119344

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
New insights into the ferroptosis and immune infiltration in endometriosis: a bioinformatics-based analysis DOI Creative Commons

Lusha Liu,

Feifei Han,

Naiyi Du

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 13, 2025

Ferroptosis, a recently discovered iron-dependent cell death, is linked to various diseases but its role in endometriosis still not fully understood. In this study, we integrated microarray data of from the GEO database and ferroptosis-related genes (FRGs) FerrDb further investigate regulation ferroptosis impact on immune microenvironment. WGCNA identified modules, annotated by GO & KEGG. MNC algorithm pinpointed hub FRGs. Cytoscape construct ceRNA network, ROC curves evaluated diagnostic efficacy Consensus cluster analysis subclusters, CIBERSORT assessed infiltration these subclusters. Finally, RT-qPCR validated FRG expression clinical tissues. We two modules endometriosis, enrichment analysis, they are closely with autophagy, mTOR, oxidative stress, FOXO pathways. Furthermore, 10 FRGs, curve showed better predictive ability for diagnosing. confirmed that tissue FRGs was mostly consistent results. Subsequently, developed network based 4 (BECN1, OSBPL9, TGFBR1, GSK3B). Next, subclusters stage. Importantly, illustrated levels cells checkpoint were significantly different Specifically, subcluster stage III-IV more inclined immunosuppressive Our study may jointly promote progression remodeling microenvironment, offering new insights into pathogenesis therapeutics.

Язык: Английский

Процитировано

0
Correlation of ferritin and glutathione peroxidase 4 (GPX4) level as a marker of ferroptosis process in endometrioma DOI Creative Commons

Rina Nulianti,

Hartanto Bayuaji, Mulyanusa Amarullah Ritonga

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 5, 2025

Abstract Endometriosis is a chronic inflammatory disease characterized by the presence of endometrial-like tissues (glands and stroma) located outside uterine cavity. The pathophysiology this condition remains incompletely understood. Local bleeding inflammation within endometriosis lesions, which occur in an environment rich iron, reactive oxygen species (ROS), free radicals, can disturb balance iron peritoneal This disruption trigger oxidative injury response, leading to ferroptosis, particularly endometrioma phenotype. research utilized observational analytical method with cross-sectional design examine relationship between ferritin GPx4 levels assessing them simultaneously. data collection occurred at Dr. Hasan Sadikin Hospital, Cibabat Bandung Kiwari Limijati Women Children Hospital Bandung. Observational analytic were gathered February July 2023 from female patients diagnosed who underwent either laparoscopic or laparotomy surgery, for therapeutic diagnostic reasons. There 58 met inclusion exclusion criteria study. A significant correlation was observed glutathione peroxidase 4 (GPx4) coefficient -0.600 ( p < 0.001). However, there no both severity (based on Association Gynecologic Laparoscopists staging). levels.

Язык: Английский

Процитировано

0
Angiopoietin‐2 regulates the phenotypic switch of vascular smooth muscle cells DOI

Xiaowen Gan,

Shenjiao Lu,

Fen Ning

и другие.

The FASEB Journal, Год журнала: 2025, Номер 39(5)

Опубликована: Март 3, 2025

Abstract During uterine spiral artery remodeling, vascular smooth muscle cells (VSMCs) become disorganized and undergo phenotypic switching from a contractile to more synthetic phenotype. We have previously reported that natural killer induce this VSMC by secreting angiopoietin‐2 (Ang‐2). Here, we identified the specific mechanisms which Ang‐2 plays role in phenomenon. VSMCs isolated human umbilical arteries were used as an vitro model investigate of switching. Human decidua tissue preeclamptic control pregnancies was collected compare expression levels related proteins. induced phenotype evidenced decreased marker expression, increased proliferation migration, altered cytoskeleton. expressed integrin β6 interacted directly with phosphorylation FAK (S910 Y397), AKT (S473), mTOR (S2448). Knockdown recovered calponin loss resulted lower EZH2 abundance. Inhibition both attenuated Ang‐2‐induced inhibition LC3 II/LC3 I ratio ATG7 proliferation. Lipid peroxidation ferrostatin‐1 or IL‐8 receptor antagonist navarixin inhibited migration. secretion significantly lipid inhibition. In decidua, there unremodeled arteries, abundance dysregulated. dysregulation may disrupt remodeling contribute preeclampsia. be novel therapeutic target for treatment pregnancy complications affected incomplete remodeling.

Язык: Английский

Процитировано

0