Functional & Integrative Genomics, Год журнала: 2024, Номер 24(2)
Опубликована: Март 5, 2024
Язык: Английский
Cell Research, Год журнала: 2024, Номер 34(9), С. 609 - 629
Опубликована: Июль 25, 2024
The advent of high-throughput sequencing uncovered that our genome is pervasively transcribed into RNAs are seemingly not translated proteins. It was also found non-coding RNA transcripts outnumber canonical protein-coding genes. This mindboggling discovery prompted a surge in research started unraveling the functional relevance these new genetic units, shaking classic definition "gene". While revolution still taking place, polysome/ribosome profiling and mass spectrometry analyses revealed peptides can be from non-canonical open reading frames. Therefore, it becoming evident coding vs dichotomy way blurrier than anticipated. In this review, we focus on several examples which binary classification genes outdated, since same bifunctional gene expresses both products. We discuss implications intricate usage terms molecular mechanisms expression biological outputs, often concordant, but surprisingly discordant. Finally, methodological caveats associated with study genes, highlight opportunities challenges therapeutic exploitation intricacy towards development anticancer therapies.
Язык: Английский
Процитировано
19
Non-coding RNA Research, Год журнала: 2024, Номер 9(3), С. 831 - 852
Опубликована: Март 31, 2024
Non-coding RNAs (ncRNAs) are a diverse group of functional RNA molecules that lack the ability to code for proteins. Despite missing this traditional role, ncRNAs have emerged as crucial regulators various biological processes and been implicated in development progression many diseases, including cancer. MicroRNAs (miRNAs) long non-coding (lncRNAs) two prominent classes key players cancer pathophysiology. In particular, miR-21 has reported exhibit oncogenic roles forms human cancer, prostate, breast, lung, colorectal context, overexpression is closely associated with tumor proliferation, growth, invasion, angiogenesis, chemoresistance, whereas inactivation linked regression most tumor-related processes. Accordingly, modulator canonical pathways such PTEN/PI3K/Akt, Wnt/β-catenin, STAT, p53, MMP2, MMP9. Moreover, interplays between lncRNA miRNA further complicate regulatory mechanisms underlying progression. regard, several lncRNAs found interact and, by functioning competitive endogenous (ceRNAs) or sponges, can modulate tumorigenesis. This work presents discusses recent findings highlighting pathophysiological implications miR-21-lncRNA axis occurrence, development, The data collected indicate specific lncRNAs, MEG3, CASC2, GAS5, strongly types gastric, cervical, glioma. Indeed, these well-known suppressors commonly downregulated different tumors. Conversely, modulating signaling pathways, their preventing formation development. review highlights significance potential use therapy diagnostic prognostic markers.
Язык: Английский
Процитировано
9
Non-Coding RNA, Год журнала: 2023, Номер 9(2), С. 19 - 19
Опубликована: Март 9, 2023
Liver cancer is the second leading cause of cancer-related death globally, with limited treatment options. Recent studies have demonstrated critical role long noncoding RNAs (lncRNAs) in pathogenesis liver cancers. Of note, mounting evidence has shown that lncRNA H19, an endogenous single-stranded RNA, functions as oncogene development and progression cancer, including hepatocellular carcinoma (HCC) cholangiocarcinoma (CCA), two most prevalent primary tumors adults. H19 can affect many biological processes, cell proliferation, apoptosis, invasion, metastasis by its function on epigenetic modification, H19/miR-675 axis, miRNAs sponge, drug resistance, regulation downstream pathways. In this review, we will focus relevant molecular mechanisms action pathophysiology HCC CCA. This review aims to provide valuable perspectives translational applications a potential diagnostic marker therapeutic target for disease.
Язык: Английский
Процитировано
18
Frontiers in Oncology, Год журнала: 2023, Номер 13
Опубликована: Май 23, 2023
Pancreatic cancer (PC) is one of the most dangerous diseases that threaten human life, and investigating details affecting its progression or regression particularly important. Exosomes are derivatives produced from different cells, including tumor cells other such as Tregs, M2 macrophages, MDSCs, can help growth. These exosomes perform their actions by in microenvironment, pancreatic stellate (PSCs) produce extracellular matrix (ECM) components immune responsible for killing cells. It has also been shown cell (PCC)-derived at stages carry molecules. Checking presence these molecules blood body fluids us early stage diagnosis monitoring PC. However, system cell-derived (IEXs) mesenchymal stem (MSC)-derived contribute to PC treatment. Immune part mechanisms involved surveillance cell-killing phenomenon. be modified a way antitumor properties enhanced. One methods drug loading exosomes, which significantly increase effectiveness chemotherapy drugs. In general, form complex intercellular communication network plays role developing, progressing, diagnosing, monitoring, treating cancer.
Язык: Английский
Процитировано
15
Non-Coding RNA, Год журнала: 2023, Номер 9(6), С. 70 - 70
Опубликована: Ноя. 11, 2023
Long non-coding RNAs (lncRNAs) play pivotal roles in regulating immune responses, cell differentiation, activation, and inflammatory processes. In cancer, they are gaining prominence as potential therapeutic targets due to their ability regulate checkpoint molecules immune-related factors, suggesting avenues for bolstering anti-tumor responses. Here, we explore the mechanistic insights into lncRNA-mediated modulation, highlighting impact on immunity. Additionally, discuss enhance cancer immunotherapy, augmenting effectiveness of inhibitors adoptive T therapies. LncRNAs hold promise revolutionizing treatments, inspiring further research this field with substantial clinical implications.
Язык: Английский
Процитировано
13
Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)
Опубликована: Май 8, 2024
Abstract Despite advances in treatment strategies, colorectal cancer (CRC) continues to cause significant morbidity and mortality, with mounting evidence a close link between immune system dysfunctions issued. Interleukin-2 receptor gamma (IL-2RG) plays pivotal role as common subunit the IL-2 family cytokines activates JAK-STAT pathway. This study delves into of within tumor microenvironment investigates potential microRNAs (miRNAs) that directly inhibit IL-2RG, aiming discern their impact on CRC clinical outcomes. Bioinformatics analysis revealed upregulation IL-2RG mRNA TCGA-COAD samples showed strong correlations infiltration various lymphocytes. Single-cell corroborated these findings, highlighting expression critical cell subsets. To explore miRNA involvement dysregulation, was isolated from tissues lymphocytes 258 patients 30 healthy controls, cloned pcDNA3.1/CT-GFP-TOPO vector. Human embryonic kidney lines (HEK-293T) were transfected this construct. Our research involved comprehensive miRPathDB, miRWalk, Targetscan databases identify miRNAs associated 3′ UTR human IL-2RG. The microRNA (miRNA) molecules, hsa-miR-7-5p hsa-miR-26b-5p, have been identified potent suppressors patients. Specifically, downregulation hsa-miR-26b-5p has shown result Prognostic evaluation hsa-miR-7-5p, using data patient samples, established higher lower both poorer Additionally, several long non-coding RNAs (LncRNAs), such ZFAS1, SOX21-AS1, SNHG11, SNHG16, SNHG1, DLX6-AS1, GAS5, SNHG6, MALAT1, which may act competing endogenous RNA molecules for IL2RG by sequestering shared hsa-miR-26b-5p. In summary, investigation underscores utility serum tissue biomarkers predicting prognosis while also offering promise targets immunotherapy management. Graphical
Язык: Английский
Процитировано
5
International Journal of Biological Macromolecules, Год журнала: 2022, Номер 222, С. 1538 - 1550
Опубликована: Сен. 22, 2022
Язык: Английский
Процитировано
19
Frontiers in Physiology, Год журнала: 2023, Номер 14
Опубликована: Окт. 10, 2023
Skin aging is a complex process influenced by intrinsic and extrinsic factors. Although dermatology offers advanced interventions, molecular mechanisms in skin remain limited. Competing endogenous RNAs (ceRNAs), subset of coding or non-coding RNAs, regulate gene expression through miRNA competition. Several ceRNA networks investigated up to now offer insights into wound healing. In aging, RP11-670E13.6-miR-663a-CDK4/CD6 delays senescence induced UVB radiation. Meg3-miR-93-5p-epiregulin contributes UVB-induced inflammatory damage. Predicted reveal UVA-induced photoaging mechanisms. SPRR2C sequesters miRNAs epidermal aging-associated alteration calcium gradient. H19-miR-296-5p-IGF2 regulates dermal fibroblast senescence. PVT1-miR-551b-3p-AQP3 influences photoaging. And bioinformatics analyses identify critical genes compounds for interventions. healing, MALAT1-miR-124 aids healing activating the Wnt/β-catenin pathway. Hair follicle MSC-derived H19 promotes inhibiting pyroptosis. SAN-miR-143-3p-ADD3 network rejuvenates adipose-derived mesenchymal stem cells Thus, provide valuable underpinnings offering potential therapeutic strategies further investigation. This comprehensive review serves as foundational platform future research endeavors these crucial areas dermatology.
Язык: Английский
Процитировано
11
International Journal of COPD, Год журнала: 2024, Номер Volume 19, С. 419 - 430
Опубликована: Фев. 1, 2024
Purpose: Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide, characterized by intense lung infiltrations immune cells (macrophages and monocytes). While existing studies have highlighted crucial role competitive endogenous RNA (ceRNA) regulatory network in COPD development, complexity characteristics ceRNA monocytes remain unexplored. Methods: We downloaded messenger (mRNA), microRNA (miRNA), long noncoding (lncRNA) microarray data from GSE146560, GSE102915, GSE71220 Gene Expression Omnibus (GEO) database. This was used to identify differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs), lncRNAs (DElncRNAs). Predicted that bind DElncRNAs were intersected with DEmiRNAs, forming a set intersecting miRNAs. then predict potential binding mRNAs, DEmRNAs, underwent functional enrichment analysis using R software STRING The resulting triple hub genes constructed Cytoscape. Comparative Toxicomics Database (CTD) utilized for correlation predictions, ROC curve assessed diagnostic accuracy. Results: Our study identified 5 lncRNAs, 4 miRNAs, 149 as expressed. A lncRNA-miRNA-mRNA constructed, selected through analysis. Enrichment terms related cell movement gene expression regulation. established LINC00482-has-miR-6088-PRRC2B relevance COPD. demonstrated value these genes. Moreover, positive between LINC00482 PRRC2B observed PBMCs. CTD database indicated their involvement inflammatory responses. Conclusion: In summary, our not only pivotal peripheral blood mononuclear (PBMCs) but also network. contributes understanding pathophysiological processes bioinformatics analysis, expanding knowledge COPD, providing foundation therapeutic targets Keywords: PBMCs, ceRNA, PRRC2B,
Язык: Английский
Процитировано
4
Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12
Опубликована: Авг. 15, 2024
Liver cancer is one of the most lethal gastrointestinal malignancies. Emerging evidence has underscored pivotal role long non-coding RNAs (lncRNAs) in tumorigenesis, with ST8SIA6-AS1 identified as a novel oncogenic lncRNA contributing to liver progression. consistently upregulated hepatic tissues and strongly associated unfavorable prognosis. Moreover, it demonstrates high diagnostic efficacy detecting HCC. involved various cellular processes including proliferation, migration, invasion, primarily through its function competing endogenous RNA (ceRNA), thereby facilitating hepatocarcinogenesis disease advancement. This review provides detailed examination molecular functions regulatory mechanisms hepatocellular carcinoma (HCC) highlights potential promising biomarker for cancer, aiming propel development innovative therapeutic strategies HCC management.
Язык: Английский
Процитировано
4