Comprehensive single-cell transcriptomic profiling reveals molecular subtypes and prognostic biomarkers with implications for targeted therapy in esophageal squamous cell carcinoma DOI Creative Commons
Dengfeng Zhang, Fangchao Zhao, Jing Li

и другие.

Translational Oncology, Год журнала: 2024, Номер 44, С. 101948 - 101948

Опубликована: Апрель 6, 2024

Esophageal squamous cell carcinoma (ESCC) is a genetically heterogeneous disease with poor clinical outcomes. Identification of biomarkers linked to DNA replication stress may enable improved prognostic risk stratification and guide therapeutic decision making. We performed integrated single-cell RNA sequencing computational analyses define the molecular determinants subtypes underlying ESCC heterogeneity. Single-cell was on samples analyzed using Seurat. Differential gene expression analysis used identify esophageal phenotypes. stress-related genes were intersected differential data potential genes, which generate (DRS) score. This score associated evaluated in survival analysis. Putative by Cox regression consensus clustering. Mendelian randomization assessed causal role PRKCB. High DRS survival. Four (CDKN2A, NUP155, PPP2R2A, PRKCB) displayed utility. Three identified discrete immune properties. A 12-gene signature robust performance. PRKCB overexpressed ESCC, while knockdown reduced migration. provides new insights into heterogeneity ESCC. The findings that patient Experimental validation substantiates utility our results.

Язык: Английский

Targeting tumor-associated macrophages in colon cancer: mechanisms and therapeutic strategies DOI Creative Commons

Jianqin Xiang,

Jian Wang, Huihui Xiao

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 21, 2025

Colon cancer (CC) remains a primary contributor to cancer-related fatalities worldwide, driven by difficulties in early diagnosis and constrained therapeutic options. Recent studies underscore the importance of tumor microenvironment (TME), notably tumor-associated macrophages (TAMs), fostering malignancy progression therapy resistance. Through their inherent plasticity, TAMs facilitate immunosuppression, angiogenic processes, metastatic spread, drug tolerance. In contrast M1 macrophages, which promote inflammatory tumoricidal responses, M2 support expansion dissemination exerting immunosuppressive pro-angiogenic influences. Consequently, manipulating has emerged as potential avenue enhance treatment effectiveness. This review outlines origins, polarization states, functions CC, highlights role driving advancement, surveys ongoing efforts target these cells for better patient outcomes. Emerging strategies aimed at modulating TAM - including depletion strategies, reprogramming approaches that shift M2-polarized toward an phenotype, inhibition key signaling pathways sustaining TAM-mediated immunosuppression-are currently under active investigation. These hold promise overcoming induced resistance improving immunotherapeutic efficacy CC.

Язык: Английский

Процитировано

0

The role of Treg cells in colorectal cancer and the immunotherapy targeting Treg cells DOI Creative Commons
Han‐Qing Yu, Rui Yang, Meixiang Li

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 16, 2025

Colorectal cancer (CRC) is among the most prevalent and lethal cancers globally, accounting for approximately 10% of all cases deaths. Regulatory T (Treg) cells, which accumulate in CRC tissue, suppress anti-tumor immune responses facilitate tumor progression. This review discusses Treg cell origins functions, along with mechanisms by Tregs influence development. In addition, we highlight therapeutic strategies targeting Tregs-such as checkpoint inhibitors combinatorial approaches-to enhance effector responses. A deeper understanding Treg-mediated immunosuppression may inform design more effective immunotherapies precision medicine strategies.

Язык: Английский

Процитировано

0

Arnicolide D: a multi-targeted anticancer sesquiterpene lactone—preclinical efficacy and mechanistic insights DOI

Shivaprasad Shetty Mangalpady,

Sheila I. Peña‐Corona, Fabiola V. Borbolla-Jiménez

и другие.

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2024, Номер 397(9), С. 6317 - 6336

Опубликована: Апрель 23, 2024

Язык: Английский

Процитировано

3

Zinc dampens antitumor immunity by promoting Foxp3+ regulatory T cells DOI Creative Commons

Sugandha Narayan,

Rajdeep Dalal,

Zaigham Abbas Rizvi

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Авг. 23, 2024

Introduction The role of zinc (Zn) in tumor development and immune modulation has always been paradoxical. This study redefines our understanding the impact Zn on cancer progression therapeutic strategies. Methods We investigated effects dietary levels responses. included examining both high deficient Zn, as well chelation, growth cell populations. Specifically, we analyzed frequency Foxp3 + regulatory T-cells (Tregs) identified FOXO1 Zn-mediated Tregs. Additionally, explored potential clioquinol (CQ) enhancing α -PD-1 immunotherapy responses, particularly melanoma. Results Our findings show that promotes by fostering a protumorigenic environment mediated T cells. Increased intake was found to facilitate increasing Treg frequency. In contrast, deficiency chelation tissue emerged potent drivers antitumor immunity. pinpointed master regulator governing influence Discussion These results reveal novel mechanistic insight into how influences regulation. identification key opens new avenues for biology. Furthermore, introduce promising approach showing administering significantly enhances response, revelations transform comprehension multifaceted tumorigenesis regulation, highlighting innovative possibilities therapy.

Язык: Английский

Процитировано

3

Comprehensive single-cell transcriptomic profiling reveals molecular subtypes and prognostic biomarkers with implications for targeted therapy in esophageal squamous cell carcinoma DOI Creative Commons
Dengfeng Zhang, Fangchao Zhao, Jing Li

и другие.

Translational Oncology, Год журнала: 2024, Номер 44, С. 101948 - 101948

Опубликована: Апрель 6, 2024

Esophageal squamous cell carcinoma (ESCC) is a genetically heterogeneous disease with poor clinical outcomes. Identification of biomarkers linked to DNA replication stress may enable improved prognostic risk stratification and guide therapeutic decision making. We performed integrated single-cell RNA sequencing computational analyses define the molecular determinants subtypes underlying ESCC heterogeneity. Single-cell was on samples analyzed using Seurat. Differential gene expression analysis used identify esophageal phenotypes. stress-related genes were intersected differential data potential genes, which generate (DRS) score. This score associated evaluated in survival analysis. Putative by Cox regression consensus clustering. Mendelian randomization assessed causal role PRKCB. High DRS survival. Four (CDKN2A, NUP155, PPP2R2A, PRKCB) displayed utility. Three identified discrete immune properties. A 12-gene signature robust performance. PRKCB overexpressed ESCC, while knockdown reduced migration. provides new insights into heterogeneity ESCC. The findings that patient Experimental validation substantiates utility our results.

Язык: Английский

Процитировано

1